IBRDC1 (RNF217) (NM_001286398) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C6orf172; dJ84N20.1; IBRDC1; OSTL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336707 representing NM_001286398. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCGAGGAGCAGAGCACGGTGAGCGGCGGCGGCGGGCCCCAGGAGTCGCAGACCCTGGCCAGTGGC ACTGCGGGCCACCCTGAGCCCCCGAGGCCTCAGGGGGACAGCGCCCGGGCGCCCCCGCTGCGCGCCGCC TCCGCGGAGCCGAGCGGCGGTGGCTGCGGAAGCGACTGGGGCTGCGCGGACACCAGCGCCCCAGAGCCC GCGAGGAGCCTGGGGCCCCCGGGCTGGAGTAAGAGCCGAGCACCGGCGCAGCCTGCGGGACTGGCACTC ACCGGGCCTCTCAATCCCCAGACCTTGCCACTGCAGTTGGAGCTGGAGGAGGAAGAGGAGGAAGCTGGG GATCGAAAAGAGGGAGGGGATGAACAGCAGGAGGCGCCCCCCGGCGAAGAGCTGGAGCCCAGGACCCGC GTGGGGGCCGCCGACGGACTGGTCCTGGACGTGCTGGGTCAGCGGCGCCCGTCCCTCGCCAAGAGACAA GTCTTCTGCTCCGTGTACTGCGTGGAGAGCGACCTGCCCGAGGCCCCCGCCTCGGAGCAGCTCTCGCCG CCCGCGTCGCCACCTGGGGCTCCGCCAGTGTTGAACCCTCCCAGCACCCGCTCTTCCTTCCCCAGCCCC CGACTGTCCCTCCCAACGGATTCCCTCTCCCCCGACGGCGGCAGCATCGAGCTGGAGTTCTACCTGGCG CCCGAGCCGTTCTCCATGCCCAGCCTGTTGGGAGCTCCACCCTACTCTGGCCTGGGCGGTGTAGGGGAT CCCTATGTGCCCCTCATGGTGCTGATGTGCCGGGTGTGCCTGGAAGACAAGCCCATCAAGCCCCTGCCT TGCTGCAAGAAGGCCGTGTGCGAGGAGTGCCTCAAAGTCTACCTGAGCGCCCAGGTACAACTTGGCCAA GTAGAAATCAAATGCCCCATCACAGAGTGTTTTGAATTCTTGGAAGAAACAACTGTTGTCTATAACTTA ACGCATGAAGACTCCATCAAGTATAAGTACTTCTTGGAACTTGGCCGTATTGATTCCAGCACCAAGCCA TGTCCTCAGTGCAAGCACTTTACAACCTTCAAGAAAAAAGGACATATTCCCACCCCTTCCAGATCAGAA AGCAAATACAAAATCCAGTGCCCTACCTGCCAATTCGTCTGGTGTTTTAAGTGCCACTCTCCTTGGCAT GAAGGTGTTAACTGCAAGGAGTACAAAAAAGGAGACAAATTGTTGCGTCACTGGGCCAGCGAAATTGAG CATGGGCAGAGGAATGCCCAGAAGTGTCCAAAGTGCAAGATCCACATCCAGCGAACTGAAGGATGTGAC CATATGACCTGCTCACAATGTAACACTAATTTTTGTTACCGATGTGGTGAGAGATACCGCCAGCTCCGA TTTTTTGGAGACCACACATCAAACCTCAGTATATTTGGATGCAAATATCGCTACCTCCCAGAGAGACCT CATTTAAGGAGATTAGTGCGAGGGTCAGTCTGTGCTGGAAAATTATTCATTGCACCTCTAATTATGGTT TTGGGATTGGCACTAGGGGCCATAGCGGTTGTAATCGGTTTATTTGTATTTCCTATCTATTGCCTTTGT AAAAAACAGAGAAAACGATCACGGACAGGTATGCACTGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286398
Insert Size	1629 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286398.2
RefSeq Size	11326 bp
RefSeq ORF	1629 bp
Locus ID	154214
UniProt ID	Q8TC41
Protein Families	Transmembrane
MW	59.4 kDa
Gene Summary	This protein encoded by this gene is a member of the RING1-IBR-RING24 (RBR) ubiquitin protein ligase family, and it belongs to a subfamily of these proteins that contain a transmembrane domain. This protein can interact with the HAX1 anti-apoptotic protein via its C-terminal RING finger motif, which suggests a role in apoptosis signaling. It is thought that deregulation of this gene can be a mechanism in leukemogenesis. Mutations in the region encoding the protein GXXXG motif, which appears to be necessary for protein self-association, have been found in human cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.