ZNF283 (NM_001297752) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HZF19; HZF41
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336698 representing NM_001297752. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGGACAAAAGTAAAACCCTTGGCCTTGAGGCATCCATCTTCAGAAATAATTGGAAGTGCAAAAGC ATATTCGAGGGACTAAAAGGACATCAAGAGGGATACTTCAGTCAAATGATAATCAGCTATGAAAAAATA CCTTCTTACAGAAAAAGTAAATCTCTTACTCCACATCAAAGAATTCATAATACAGAGAAATCCTATGTT TGTAAGGAATGTGGGAAGGCTTGCAGTCATGGCTCAAAACTTGTTCAACATGAGAGAACTCATACAGCT GAAAAACACTTTGAATGTAAAGAATGTGGGAAGAATTATTTAAGTGCCTATCAACTCAATGTGCATCAG AGATTTCATACTGGTGAGAAACCCTATGAGTGTAAGGAATGTGGGAAGACCTTTAGCTGGGGATCAAGC CTTGTTAAACATGAGAGAATTCACACTGGTGAGAAACCCTATGAATGTAAAGAATGTGGGAAGGCCTTT AGTCGTGGCTATCACCTTACCCAACATCAGAAAATTCATACTGGTGTGAAATCTTATAAATGTAAGGAA TGTGGGAAGGCCTTTTTTTGGGGCTCAAGCCTTGCTAAACATGAGATAATTCATACAGGTGAGAAACCT TATAAATGTAAAGAATGTGGGAAGGCCTTCAGTCGTGGCTATCAGCTTACTCAGCATCAGAAAATCCAT ACTGGTAAGAAACCTTATGAATGTAAAATATGTGGAAAGGCTTTTTGTTGGGGCTATCAACTTACTCGA CATCAGATATTTCATACTGGTGAGAAACCCTATGAATGCAAGGAATGTGGGAAGGCTTTTAATTGCGGA TCAAGTCTTATTCAACATGAAAGAATTCATACTGGTGAGAAACCTTATGAATGTAAAGAATGTGGAAAG GCCTTTAGTCGTGGCTATCACCTTTCTCAACATCAGAAAATCCATACTGGTGAGAAACCTTTTGAATGT AAGGAATGTGGGAAGGCCTTTAGTTGGGGTTCAAGCCTTGTTAAACATGAGAGAGTTCATACTGGTGAG AAATCCCATGAATGTAAAGAATGCGGAAAGACCTTTTGTAGTGGGTATCAACTTACTCGACATCAGGTA TTTCACACTGGTGAGAAACCCTATGAATGTAAGGAATGTGGGAAGGCTTTTAATTGTGGATCAAGCCTT GTTCAACATGAAAGAATCCATACAGGGGAGAAACCCTATGAATGTAAAGAATGTGGGAAGGCTTTTAGT CGTGGCTATCACCTTACTCAACATCAGAAAATTCATACCGGTGAGAAACCTTTCAAATGTAAGGAATGT GGGAAGGCCTTCAGTTGGGGTTCAAGCCTAGTTAAGCATGAGAGAGTCCATACTAATGAGAAGTCTTAT GAATGTAAAGACTGTGGGAAGGCCTTTGGTAGTGGCTATCAACTTAGTGTTCATCAGAGATTTCATACT GGTGAGAAGCTTTATCAACGTAAGGAATTCGGGAAGACCTTTACTTGTGGCTCAAAACTTGTTCATGAG AGAACTCATAGTAATGATAAACCCTACAAATATAACGAATGTGGGGAAGCCTTTCTGTGGACAACTTAC TCAAATGAGAAAATTGATACTGATGAAACCTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297752
Insert Size	1623 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297752.1
RefSeq Size	2527 bp
RefSeq ORF	1623 bp
Locus ID	284349
UniProt ID	Q8N7M2
MW	62.3 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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