ZNF238 (ZBTB18) (NM_001278196) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C2H2-171; MRD22; RP58; TAZ-1; ZNF238
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC336598 representing NM_001278196. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGTTTCCAGACCATAGTAGACATTTGCTACAGTGTCTGAGCGAGCAGAGACACCAGGGTTTTCTT TGTGACTGCACTGTTCTGGTGGGAGATGCCCAGTTCCGAGCGCACCGAGCTGTACTGGCTTCATGCAGC ATGTATTTCCACCTCTTTTACAAGGACCAGCTGGACAAAAGAGACATTGTTCATCTGAACAGCGACATT GTTACAGCCCCCGCTTTCGCTCTCCTGCTTGAATTCATGTATGAAGGGAAACTCCAGTTCAAAGACTTG CCCATTGAAGACGTGCTAGCAGCTGCCAGTTATCTCCACATGTATGACATTGTCAAAGTCTGCAAAAAG AAGCTGAAAGAGAAAGCCACCACGGAGGCAGACAGCACCAAAAAGGAAGAAGATGCTTCAAGTTGTTCG GACAAAGTCGAGAGTCTCTCCGATGGCAGCAGCCACATAGCAGGCGATTTGCCCAGTGATGAAGATGAA GGAGAAGATGAAAAATTGAACATCCTGCCCAGCAAAAGGGACTTGGCGGCCGAGCCTGGGAACATGTGG ATGCGATTGCCCTCAGACTCAGCAGGCATCCCCCAGGCTGGCGGAGAGGCAGAGCCACACGCCACAGCA GCTGGAAAAACAGTAGCCAGCCCCTGCAGCTCAACAGAGTCTTTGTCCCAGAGGTCTGTCACCTCCGTG AGGGATTCGGCAGATGTTGACTGTGTGCTGGACCTGTCTGTCAAGTCCAGCCTTTCAGGAGTTGAAAAT CTGAACAGCTCTTATTTCTCTTCACAGGACGTGCTGAGAAGCAACCTGGTGCAGGTGAAGGTGGAGAAA GAGGCTTCCTGTGATGAGAGTGATGTTGGCACTAATGACTATGACATGGAACATAGCACTGTGAAAGAA AGTGTGAGCACTAATAACAGGGTACAGTATGAGCCGGCCCATCTGGCTCCCCTGAGGGAGGACTCGGTC TTGAGGGAGCTGGACCGGGAGGACAAAGCCAGTGATGATGAGATGATGACCCCAGAGAGCGAGCGTGTC CAGGTGGAGGGAGGCATGGAGAGCAGTCTGCTCCCCTACGTCTCCAACATCCTGAGCCCCGCGGGCCAG ATCTTCATGTGCCCCCTGTGCAACAAGGTCTTCCCCAGCCCCCACATCCTGCAGATCCACCTGAGCACG CACTTCCGCGAGCAGGACGGCATCCGCAGCAAGCCCGCCGCCGATGTCAACGTGCCCACGTGCTCGCTG TGTGGGAAGACTTTCTCTTGCATGTACACCCTCAAGCGCCACGAGAGGACTCACTCGGGGGAGAAGCCC TACACATGCACCCAGTGCGGCAAGAGCTTCCAGTACTCGCACAACCTGAGCCGCCATGCCGTGGTGCAC ACCCGCGAGAAGCCGCACGCCTGCAAGTGGTGCGAGCGCAGGTTCACGCAGTCCGGGGACCTGTACAGA CACATTCGCAAGTTCCACTGTGAGTTGGTGAACTCCTTGTCGGTCAAAAGCGAAGCACTGAGCTTGCCT ACTGTCAGAGACTGGACCTTAGAAGATAGCTCTCAAGAACTTTGGAAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001278196
Insert Size	1569 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278196.1
RefSeq Size	3791 bp
RefSeq ORF	1569 bp
Locus ID	10472
UniProt ID	Q99592
Protein Families	Transcription Factors
MW	58.4 kDa
Gene Summary	This gene encodes a C2H2-type zinc finger protein which acts a transcriptional repressor of genes involved in neuronal development. The encoded protein recognizes a specific sequence motif and recruits components of chromatin to target genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform (2).

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