ADA2 (NM_001282225) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADGF; CECR1; IDGFL; PAN; SNEDS; VAIHS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336539 representing NM_001282225. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGGTGGATGGCCCATCTGAGCGGCCAGCCCTGTGCTTCTTGCTGTTGGCTGTGGCAATGTCTTTC TTCGGCTCAGCTCTATCCATAGATGAAACACGGGCGCATCTGTTGTTGAAAGAAAAGATGATGCGGCTG GGGGGGCGGCTGGTGCTGAACACCAAGGAGGAGCTGGCCAATGAGAGGCTCATGACGCTCAAAATCGCT GAGATGAAGGAGGCCATGAGGACCCTGATATTCCCACCCAGCATGCACTTTTTCCAGGCCAAGCATCTC ATTGAGAGAAGTCAAGTGTTTAATATTCTAAGGATGATGCCAAAAGGGGCTGCCTTGCACCTCCATGAC ATTGGCATCGTGACTATGGACTGGCTGGTGAGGAATGTCACCTACAGGCCTCACTGCCACATCTGTTTC ACCCCAAGGGGGATCATGCAGTTCAGATTTGCTCACCCAACTCCCCGTCCATCAGAAAAATGTTCCAAG TGGATTCTGCTGGAGGATTATCGGAAGCGGGTGCAGAACGTCACTGAGTTTGATGACAGCTTGCTGAGG AATTTCACTCTGGTGACCCAGCACCCGGAGGTGATTTACACAAACCAAAATGTTGTCTGGTCGAAATTT GAAACCATCTTCTTCACCATCTCTGGTCTCATCCATTACGCACCAGTGTTCAGAGACTATGTCTTCCGG AGCATGCAGGAGTTCTACGAGGACAACGTGCTCTACATGGAGATCAGAGCCAGGCTGCTGCCGGTGTAT GAGCTCAGTGGAGAGCACCATGACGAAGAGTGGTCAGTGAAGACTTACCAGGAAGTAGCTCAGAAGTTT GTGGAAACTCACCCTGAGTTTATTGGAATCAAAATCATTTATTCGGATCACAGATCCAAAGATGTGGCT GTCATCGCAGAATCCATCCGAATGGCCATGGGGCTCCGAATCAAGTTCCCCACGGTGGTGGCAGGGTTT GACCTGGTGGGGCATGAGGACACTGGCCACTCCTTGCATGACTACAAGGAAGCTCTGATGATCCCCGCC AAGGATGGCGTTAAGCTGCCTTACTTCTTCCACGCCGGAGAAACAGACTGGCAGGGTACTTCCATAGAC AGGAACATTCTGGATGCTCTGATGCTGAACACTACCAGAATCGGCCATGGATTTGCTTTGAGCAAACAC CCCGCAGTCAGGACTTACTCCTGGAAAAAGGACATCCCCATAGAAGTCTGTCCCATCTCTAACCAGGTG CTGAAACTGGTGTCTGACTTGAGGAACCACCCTGTAGCCACTCTGATGGCCACTGGGCACCCCATGGTG ATCAGCTCTGATGACCCAGCTATGTTTGGTGCCAAAGGCTTGTCCTATGATTTCTATGAGGTCTTCATG GGCATTGGGGGGATGAAGGCTGACCTGAGGACCCTCAAACAGCTGGCCATGAACTCTATCAAGTACAGT ACCCTGTTGGAGAGTGAGAAAAATACTTTCATGGAAATCTGGAAGAAGAGATGGGATAAGTTCATAGCA GATGTGGCTACAAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282225
Insert Size	1536 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282225.1
RefSeq Size	4510 bp
RefSeq ORF	1536 bp
Locus ID	51816
UniProt ID	Q9NZK5
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
MW	58.9 kDa
Gene Summary	This gene encodes a member of a subfamily of the adenosine deaminase protein family. The encoded protein is one of two adenosine deaminases found in humans, which regulate levels of the signaling molecule, adenosine. The encoded protein is secreted from monocytes undergoing differentiation and may regulate cell proliferation and differentiation. This gene may be responsible for some of the phenotypic features associated with cat eye syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (a). Both variants 3 and 4 encode the same isoform.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.