ZMAT1 (NM_001282400) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336293 representing NM_001282400. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAACCTATGTTTGCCATATTTGTAGTATTGCTTTTACATCTTTAGATATGTTCCGGTCCCACATG CAAGGAAGTGAACATCAAATTAAAGAATCCATTGTTATCAATCTAGTGAAGAATTCAAGGAAGACACAA GACTCTTACCAAAATGAGTGTGCAGATTACATCAATGTGCAGAAAGCCAGAGGACTAGAGGCCAAGACT TGTTTCAGAAAGATGGAAGAGAGTTCTTTGGAAACCCGTAGATACAGAGAAGTGGTCGATTCCAGACCC AGACATAGAATGTTTGAACAAAGACTCCCATTTGAGACTTTCCGGACATACGCAGCACCATACAATATT TCACAAGCAATGGAAAAGCAGTTACCTCATTCAAAGAAGACATATGACTCTTTCCAAGATGAACTTGAA GATTACATCAAAGTACAGAAAGCCAGAGGACTAGATCCAAAGACTTGTTTCAGAAAGATGAGAGAGAAC TCTGTGGATACTCATGGGTACAGAGAAATGGTTGATTCTGGACCCAGATCAAGAATGTGTGAGCAAAGA TTTTCACATGAGGCTTCCCAGACCTACCAACGACCATACCATATTTCACCAGTGGAAAGCCAGTTACCT CAGTGGCTACCAACCCATTCAAAGAGGACATATGATTCTTTCCAAGATGAACTTGAAGATTACATAAAA GTGCAGAAAGCCAGAGGACTAGAGCCAAAAACTTGTTTCAGAAAGATAGGAGATAGCTCTGTAGAAACA CACAGGAACAGAGAAATGGTTGATGTCAGACCCAGACATAGAATGTTGGAGCAAAAGCTCCCATGTGAG ACTTTCCAGACCTATTCAGGACCATATAGTATTTCACAAGTAGTGGAAAACCAGTTACCTCATTGCTTA CCAGCTCATGATAGCAAACAGAGACTAGATTCTATTAGCTACTGTCAACTCACCAGAGACTGTTTCCCA GAAAAACCAGTACCCTTGAGCCTTAATCAGCAAGAAAATAACTCTGGCTCATACAGTGTAGAATCTGAA GTTTACAAGCACCTCTCTTCAGAAAACAATACTGCTGACCATCAAGCAGGTCATAAACGGAAACATCAG AAGAGAAAACGACACCTAGAAGAAGGCAAAGAAAGGCCAGAGAAAGAGCAGTCCAAGCATAAAAGGAAA AAGAGTTATGAAGATACAGATTTAGACAAAGACAAGAGCATCAGACAAAGGAAAAGAGAGGAGGATAGA GTCAAGGTCAGTTCAGGAAAGCTTAAGCATCGAAAAAAGAAAAAAAGCCATGATGTACCCTCCGAGAAA GAAGAACGTAAGCACAGGAAAGAGAAAAAGAAATCTGTTGAAGAAAGGACAGAAGAGGAAATGCTTTGG GATGAGTCTATTCTTGGATTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282400
Insert Size	1404 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282400.1
RefSeq Size	4045 bp
RefSeq ORF	1404 bp
Locus ID	84460
UniProt ID	Q5H9K5
MW	55.6 kDa
Gene Summary	This gene encodes a protein containing Cys2-His2 (C2H2)-type zinc fingers, which are similar to those found in the nuclear matrix protein matrin 3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) uses an alternate 5' exon structure and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (4) with a shorter N-terminus, compared to isoform 1. Both variants 2 and 4 encode the same isoform (4).

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