PGM3 (NM_001199918) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AGM1; IMD23; PAGM; PGM 3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336145 representing NM_001199918. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGGCACCATCCTGGGAGGAACATGCCACCTGTTTAGCAAATGCTGAGGAACAAGATATGCAGAGA GTGCTTATTGACATCAGCGAGAAAGAAGCTGTGAATCTGCAACAAGATGCCTTTGTAGTTATTGGTAGA GATACCAGGCCCAGCAGTGAGAAACTTTCACAATCTGTAATAGATGGTGTGACTGTTCTAGGAGGTCAA TTCCATGATTATGGCTTGTTAACAACACCCCAGCTGCACTACATGGTGTATTGTCGAAACACGGGTGGC CGATATGGAAAGGCAACTATAGAAGGTTACTACCAGAAACTCTCTAAGGCTTTTGTGGAACTCACCAAA CAGGCTTCTTGCAGTGGAGATGAATACAGATCACTTAAGGTTGACTGTGCAAATGGCATAGGGGCCCTG AAGCTAAGGGAAATGGAACACTACTTCTCACAGGGCCTGTCAGTTCAGCTGTTTAATGATGGGTCCAAG GGCAAACTCAATCATTTATGTGGAGCTGACTTTGTGAAAAGTCATCAGAAACCTCCACAGGGAATGGAA ATTAAGTCCAATGAAAGATGCTGTTCTTTTGATGGAGATGCAGACAGAATTGTTTATTACTACCATGAT GCAGATGGCCACTTTCATCTCATAGATGGAGACAAGATAGCAACGTTAATTAGCAGTTTCCTTAAAGAG CTCCTGGTGGAGATTGGAGAAAGTTTGAATATTGGTGTTGTACAAACTGCATATGCAAATGGAAGTTCA ACACGGTATCTTGAAGAAGTTATGAAGGTACCTGTCTATTGCACTAAGACTGGTGTAAAACATTTGCAC CACAAGGCTCAAGAGTTTGACATTGGAGTTTATTTTGAAGCAAATGGGCATGGCACTGCACTGTTTAGT ACAGCTGTTGAAATGAAGATAAAACAATCAGCAGAACAACTGGAAGATAAGAAAAGAAAAGCTGCTAAG ATGCTTGAAAACATTATTGACTTGTTTAACCAGGCAGCTGGTGATGCTATTTCTGACATGCTGGTGATT GAAGCAATCTTGGCTCTGAAGGGCTTGACTGTACAACAGTGGGATGCTCTCTATACAGATCTTCCAAAC AGACAACTTAAAGTTCAGGTTGCAGACAGGAGAGTTATTAGCACTACCGATGCTGAAAGACAAGCAGTT ACACCCCCAGGATTACAGGAGGCAATCAATGACCTGGTGAAGAAGTACAAGCTTTCTCGAGCTTTTGTC CGGCCCTCTGGTACAGAAGATGTCGTCCGAGTATATGCAGAAGCAGACTCACAAGTAAGAAAGTGCAGA TCACCTTGCACATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001199918
Insert Size	1326 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199918.1
RefSeq Size	5905 bp
RefSeq ORF	1326 bp
Locus ID	5238
Protein Pathways	Amino sugar and nucleotide sugar metabolism
MW	49.1 kDa
Gene Summary	This gene encodes a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in a shorter isoform (3) with distinct N- and C-termini, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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