MACO1 (NM_001282564) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MACOILIN; TMEM57
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336123 representing NM_001282564. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGCGGCGGAACGCCGACTGCAGTAAGCTCCGCCGCCCCCTAAAGCGGAACCGGATCACCGAGGGC ATTTACGGCAGTACATTTTTATACCTGAAATTCCTGGTGGTGTGGGCACTTGTCCTCCTAGCAGATTTT GTCCTGGAGTTCAGATTTGAATACCTGTGGCCATTCTGGCTTTTCATCAGAAGCGTCTATGATTCCTTC AGATACCAGGGACTGGCCTTCTCAGTATTTTTTGTTTGTGTAGCATTCACGTCAAATATAATATGCCTG CTGTTCATCCCCATACAGTGGCTTTTTTTTGCTGCTAGCACATATGTATGGGTTCAGTACGTATGGCAC ACAGAAAGGGGAGTGTGTTTGCCTACAGTGTCTCTCTGGATCCTCTTTGTTTATATTGAAGCAGCCATT AGATTTAAAGATCTCAAAAACTTTCATGTAGACCTTTGTCGTCCATTTGCTGCTCACTGGCTGGAACAA GACATTAAAAAGTTAAAGGCTGACCTGCAAGCCAGCAGACAAGTGGAACAAGAGCTCCGCAGTCAGATC AGCTCCCTTTCGAGCACCGAGCGAGGGATCCGCTCAGAAATGGGCCAGCTTCGGCAGGAGAACGAGCTG CTGCAGAACAAGTTACATAATGCTGTGCAAATGAAGCAAAAAGACAAGCAGAATATCAGCCAGTTGGAG AAAAAGCTAAAAGCTGAGCAGGAAGCCCGAAGTTTTGTAGAGAAACAGTTAATGGAAGAGAAAAAGAGG AAGAAGTTAGAAGAAGCCACTGCTGCCCGGGCTGTTGCGTTTGCTGCTGCATCTAGGGGAGAATGCACC GAAACCTTACGGAATCGGATCAGAGAACTAGAAGCAGAGGGCAAGAAGCTCACGATGGACATGAAGGTG AAAGAAGACCAAATCAGAGAACTAGAACTAAAAGTCCAGGAGCTTCGGAAATATAAGGAAAATGAGAAG GACACTGAGGTGTTAATGTCAGCCCTCTCAGCCATGCAAGACAAAACACAGCACCTGGAGAACAGCTTA AGTGCAGAGACGAGAATCAAGCTGGACCTGTTCTCCGCACTGGGCGATGCAAAGCGGCAGCTCGAGATT GCCCAAGGACAAATCCTTCAGAAAGATCAGGAAATCAAGGACCTAAAACAGAAGATAGCCGAAGTCATG GCCGTCATGCCCAGCATAACATACAGTGCCGCCACCAGCCCCCTGAGCCCTGTTTCCCCCCACTACTCT TCCAAATTTGTGGAGACCAGCCCCTCTGGACTTGACCCCAATGCCTCTGTTTACCAGCCCCTGAAGAAA TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282564
Insert Size	1314 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282564.1
RefSeq Size	3312 bp
RefSeq ORF	1314 bp
Locus ID	55219
UniProt ID	Q8N5G2
Protein Families	Transmembrane
MW	50.8 kDa
Gene Summary	Plays a role in the regulation of neuronal activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two internal exons, resulting in the loss of an in-frame segment in the central coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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