WSB2 (NM_001278557) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SBA2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336017 representing NM_001278557. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGGGTAGACAGGGAGAGCAGGTTTCTCAGGGGGACGGGGACAGGCGAGGCTGTCGCCGTAGAGGAA CCGCTGCTGCTGGCCGAACTCAAGCCCGGGCGCCCCCACCAGTTTGATTGGAAGTCCAGCTGTGAAACC TGGAGCGTCGCCTTCTCCCCAGATGGCTCCTGGTTTGCTTGGTCTCAAGGACACTGCATCGTCAAACTG ATCCCCTGGCCGTTGGAGGAGCAGTTCATCCCTAAAGGGTTTGAAGCCAAAAGCCGAAGTAGCAAAAAT GAGACGAAAGGGCGGGGCAGCCCAAAAGAGAAGACGCTGGACTGTGGTCAGATTGTCTGGGGGCTGGCC TTCAGCCCGTGGCCTTCCCCACCCAGCAGGAAGCTCTGGGCACGCCACCACCCCCAAGTGCCCGATGTC TCTTGCCTGGTTCTTGCTACGGGACTCAACGATGGGCAGATCAAGATCTGGGAGGTGCAGACAGGGCTC CTGCTTTTGAATCTTTCCGGCCACCAAGATGTCGTGAGAGATCTGAGCTTCACACCCAGTGGCAGTTTG ATTTTGGTCTCCGCGTCACGGGATAAGACTCTTCGCATCTGGGACCTGAATAAACACGGTAAACAGATT CAAGTGTTATCGGGCCACCTGCAGTGGGTTTACTGCTGTTCCATCTCCCCAGACTGCAGCATGCTGTGC TCTGCAGCTGGAGAGAAGTCGGTCTTTCTATGGAGCATGAGGTCCTACACGTTAATTCGGAAGCTAGAG GGCCATCAAAGCAGTGTTGTCTCTTGTGACTTCTCCCCCGACTCTGCCCTGCTTGTCACGGCTTCTTAC GATACCAATGTGATTATGTGGGACCCCTACACCGGCGAAAGGCTGAGGTCACTCCACCACACCCAGGTT GACCCCGCCATGGATGACAGTGACGTCCACATTAGCTCACTGAGATCTGTGTGCTTCTCTCCAGAAGGC TTGTACCTTGCCACGGTGGCAGATGACAGACTCCTCAGGATCTGGGCCCTGGAACTGAAAACTCCCATT GCATTTGCTCCTATGACCAATGGGCTTTGCTGCACATTTTTTCCACATGGTGGAGTCATTGCCACAGGG ACAAGAGATGGCCACGTCCAGTTCTGGACAGCTCCTAGGGTCCTGTCCTCACTGAAGCACTTATGCCGG AAAGCCCTTCGAAGTTTCCTAACAACTTACCAAGTCCTAGCACTGCCAATCCCCAAGAAAATGAAAGAG TTCCTCACATACAGGACTTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278557
Insert Size	1266 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278557.1
RefSeq Size	2798 bp
RefSeq ORF	1266 bp
Locus ID	55884
UniProt ID	Q9NYS7
Protein Families	Druggable Genome
MW	47.2 kDa
Gene Summary	This gene encodes a member of the WD-protein subfamily. The encoded protein contains five WD-repeats spanning most of the protein and an SOCS box in the C-terminus. The SOCS box may act as a bridge between specific substrate-binding domains and E3 ubiquitin protein ligases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] Transcript Variant: This variant (1) encodes the longest isoform (1).

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