FAM154A (SAXO1) (NM_001287049) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C9orf138; FAM154A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335925 representing NM_001287049. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGGCCTGACTACATCAAGGAGAGATTTTGGGCCTCACAAAGTGGCACCAGTGAAGGTCCACCAG TATGACCAGTTCGTCCCGAGTGAAGAGAATATGGATTTGCTCACGACGTATAAGAAAGATTACAATCCC TACCCTGTCTGTCGAGTGGACCCCATCAAACCTCGGGACAGTAAATATCCATGTAGCGACAAGATGGAG TGTTTGCCTACTTATAAAGCTGATTATTTGCCTTGGAACCAACCAAGGCGAGAGCCACTTCGTCTGGAA CACAAATACCAGCCGGCATCAGTCAGGTTTGATAACAGAACCACACACCAGGACGATTACCCCATAAAA GGCCTTGTGAAGACCATAAGCTGTAAACCTCTGGCCATGCCAAAGCTCTGTAACATCCCCTTGGAGGAT GTGACTAACTACAAGATGAGCTATGTGGCCCACCCCGTGGAGAAGCGCTTTGTGCATGAAGCAGAGAAG TTCAGGCCCTGTGAAATCCCCTTTGAAAGCCTTACCACTCAAAAACAATCCTACCGGGGCCTGATGGGG GAGCCTGCCAAGAGCTTGAAACCTCTAGCCAGGCCTCCTGGGCTAGACATGCCTTTCTGTAACACCACT GAGTTTCGAGATAAGTACCAAGCTTGGCCAATGCCCCGGATGTTCTCCAAAGCTCCCATCACCTACGTC CCTCCCGAAGACAGGATGGATCTTCTGACAACAGTGCAGGCCCATTACACATGCCCTAAGGGTGCCCCA GCTCAGTCCTGCCGACCTGCACTTCAGATTAAGAAGTGCGGTCGCTTTGAAGGCTCTTCCACCACCAAG GATGACTACAAGCAGTGGTCCAGCATGCGCACAGAGCCAGTCAAGCCCGTTCCCCAGCTGGACTTGCCC ACCGAGCCCCTGGACTGCCTGACCACCACTCGGGCCCACTATGTGCCCCACCTGCCTATCAATACCAAA AGCTGTAAGCCTCATTGGTCTGGCCCTCGAGGAAATGTCCCTGTGGAAAGCCAGACCACCTACACCATC AGCTTTACTCCCAAGGAAATGGGCAGGTGCCTAGCTTCATATCCTGAGCCTCCTGGCTACACCTTTGAG GAAGTGGATGCTTTGGGTCACAGGATATACAAACCAGTTTCCCAGGCAGGCTCTCAGCAGAGCAGCCAT CTTTCTGTAGATGATTCAGAAAACCCCAACCAGAGGGAGTTGGAAGTGTTAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287049
Insert Size	1230 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287049.1
RefSeq Size	1941 bp
RefSeq ORF	1230 bp
Locus ID	158297
UniProt ID	Q8IYX7
MW	46.8 kDa
Gene Summary	May play a role in the regulation of cilium length. Stabilizes microtubules at low temperature.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate 5' terminal exon and it thus differs in the 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.

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