PGAP4 (NM_001303107) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C9orf125; TMEM246
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC335896 representing NM_001303107. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCACTTCAACCTCTCCAGCTGCCATGCTCCTCCGGAGGCTGCGGCGACTCTCCTGGGGCAGCACT GCTGTCCAGCTCTTCATCCTAACAGTGGTGACGTTTGGCCTGCTGGCCCCCCTGGCCTGTCACCGACTT CTACACTCTTACTTCTATCTGCGCCATTGGCATCTGAACCAAATGAGCCAAGAGTTCCTGCAGCAAAGC TTGAAAGAGGGTGAGGCTGCCCTCCACTATTTTGAGGAGCTTCCCTCTGCCAATGGCTCAGTGCCCATT GTCTGGCAGGCCACCCCCCGGCCCTGGCTGGTGATCACCATCATCACTGTGGACAGGCAGCCTGGCTTC CACTACGTCCTGCAGGTTGTGTCCCAGTTCCACCGGCTTCTTCAGCAATGTGGCCCCCAGTGCGAGGGG CACCAACTCTTCCTGTGCAACGTGGAGCGTAGTGTGAGCCATTTTGATGCCAAGTTGCTCTCCAAGTAT GTCCCTGTGGCCAATCGCTATGAGGGCACTGAGGATGATTATGGTGATGACCCTTCGACCAACTCGTTT GAGAAAGAGAAGCAGGACTATGTCTATTGCCTGGAGTCATCCCTGCAGACCTACAACCCAGACTACGTC CTGATGGTAGAAGACGATGCTGTACCAGAAGAGCAGATCTTCCCAGTCTTGGAGCACCTTCTGCGGGCT CGCTTCTCTGAGCCACATCTCAGAGATGCCCTTTATCTCAAGCTGTATCACCCCGAGAGGCTCCAGCAC TACATCAATCCAGAGCCCATGCGGATCCTGGAATGGGTTGGTGTAGGCATGTTGCTGGGGCCCTTACTA ACCTGGATATACATGAGGTTTGCCAGCCGCCCAGGGTTTAGCTGGCCTGTAATGCTCTTCTTCTCCCTG TATAGCATGGGTCTGGTGGAGCTGGTGGGTCGGCACTATTTCCTGGAACTGCGGCGGCTGAGTCCTTCC CTGTACAGTGTGGTTCCTGCCTCTCAGTGTTGCACCCCAGCCATGCTCTTCCCGGCACCTGCGGCCCGC CGGACCCTCACCTACCTGTCCCAAGTGTACTGCCACAAGGGCTTTGGCAAGGACATGGCACTGTACTCG CTGTTGAGGGCCAAGGGAGAGAGGGCCTATGTAGTGGAGCCGAACCTCGTGAAACACATCGGGCTCTTC TCCAGTCTCCGGTACAACTTTCATCCCAGTCTCCTCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001303107
Insert Size	1212 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303107.1
RefSeq Size	4348 bp
RefSeq ORF	1212 bp
Locus ID	84302
UniProt ID	Q9BRR3
Protein Families	Transmembrane
MW	46.6 kDa
Gene Summary	Golgi-resident glycosylphosphatidylinositol (GPI)-N-acetylgalactosamine transferase involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins (PubMed:29374258). Required for the initial step of GPI-GalNAc biosynthesis, transfers GalNAc to GPI in the Golgi after fatty acid remodeling by PGAP2 (PubMed:29374258).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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