CCDC125 (NM_001297697) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KENAE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335792 representing NM_001297697. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTAAAAACTGAACTTGAGGCATCTCAAAGACAACTCAGAGGTAAAGAGGAAGCATTGAAAATTCTT CAAAGCATGGCAATACTGGGCAAAGCCACAAGTCATACGCAGGCAGTGCTTCAAAAAACTATGGAACAA AACAGATCCTTGGAGAAGGAAATAAATGCCTTGCAGTGGGAAATAGAATTTGATCATAATAGATTTAAA AATATAGAGGAATCTTGGATCCAAAAATATGACAGGCTAAACTGTGAAAATGCAGTCCTCAAAGAGAAT TTGAAAGTGAAAACAGAAGAAATTAAAATGCTGAAGTCTGACAATGCAGTTTTGAATCAACGGTATTTG GAGGCCCTCGCCATGCTTGATATCAAACAGCAGAAGATGGCTCAGGAAAACATGTGCTGTGATAAAAGT GGCTTTGCAGAGGCTTCAGGTCTTGAGCTTGCGGTCCTCGGAGCCTGCCTTTGTCATGGGCCCGGAGGG AACCCCTGTTCTTGTGCCAGAATGGCAGCATCCACTCGGAAACTGCTTCTTCAGCTCAAACAAGAGTTG GAAATTTTGCAGAAGAGTAAAGAAGAAGCTTACGTGATGGCAGATGCTTTCAGAATTGCATTTGAGCAA CAATTAATGAGAAAAAATGACCAGGCACTACAATTGACACAAATGGATAAAATGCATAAAAAAGCAACA AAATGGATGAATTGGAAGCACCTTAAAGAGGATGGATTTCCATCACCAAGGAGTAAGAAGACCTTCGGG CAGAGACTGTTGGGTATGCTCCCTTCAGAAAACAGTTCTAAGAGGATGGAAGACCAGGACAGTCCTCAA GAGGTCCTTAAGATGCTCATAGATTTGCTTAATGATAAAGAAGAAGCTTTGGCTCATCAAAGAAAAGTT AGCTACATGCTTGCTCGGGCATTGGAAGACAAAGACACTGCTTCAAACGAGAATAAAGAAAAAAATCCT ATAAAAGAGAATTTCCCTTTCAACAACCCCTGGCGTAAGACTTCAGAATTCTCTGTTTTGGGTGATCCT ATACATTCAAGTGTCTGCATTTTAAATTCTGTGGGCTGCATTTGTTCAATCCAGCACTCTCAAATAGAT CCAAACTATAGAACTCTTAAAAGATCCCATTCTTTGCCATCAAGTATCATATTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297697
Insert Size	1161 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297697.1
RefSeq Size	3528 bp
RefSeq ORF	1161 bp
Locus ID	202243
UniProt ID	Q86Z20
MW	44.2 kDa
Gene Summary	May be involved in the regulation of cell migration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has an alternate 5' UTR exon, lacks an internal segment and has an alternate splice site in the 5' region, compared to variant 1. The translation initiation is at a downstream AUG start codon, and the resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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