Znf206 (ZSCAN10) (NM_001282415) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZFP206; ZNF206
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335786 representing NM_001282415. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGCACTCACACGGACGAGCGGCCGCACGCCTGCCACCTGTGCGGCCACCGCTTCCGCCAGAGCTCG CACCTGAGCAAGCACCTGCTGACCCACTCCTCCGAACCCGCCTTCCTGTGCGCAGAGTGCGGCCGCGGC TTCCAGCGCCGCGCCAGCCTTGTGCAGCACCTGCTGGCGCACGCCCAGGACCAGAAGCCGCCCTGCGCT CCTGAGAGTAAGGCCGAAGCGCCGCCACTGACCGATGTCCTGTGCTCCCACTGCGGCCAGAGCTTCCAG CGCCGCTCCAGCCTCAAGCGCCACCTGCGGATCCACGCCAGGGACAAGGACCGCCGGTCCTCCGAAGGC TCCGGCAGCCGCCGCCGGGACTCCGACCGGAGGCCCTTCGTGTGCAGCGACTGCGGCAAGGCCTTCCGG CGCAGCGAGCACCTGGTGGCCCACCGGAGGGTGCACACGGGCGAGCGGCCCTTCTCCTGCCAGGCTTGC GGCCGCAGCTTCACGCAGAGCTCGCAGCTGGTCAGCCACCAACGGGTGCACACGGGCGAGAAGCCCTAC GCCTGTCCGCAGTGCGGGAAGCGCTTTGTGCGCCGGGCCAGCCTTGCCCGCCACCTGCTGACCCACGGT GGCCCTCGGCCCCACCACTGCACCCAGTGCGGGAAGAGTTTCGGCCAGACCCAGGATCTGGCCCGCCAC CAGCGCAGCCACACGGGCGAGAAGCCCTGCCGCTGCAGCGAGTGCGGTGAGGGCTTCAGCCAGAGCGCC CACCTGGCGCGCCACCAGCGCATCCACACAGGGGAGAAGCCCCACGCCTGCGACACCTGCGGCCACCGT TTCCGCAATAGCTCCAACCTGGCCCGCCATCGCCGCAGCCACACGGGCGAGCGGCCCTACAGCTGTCAG ACGTGCGGTCGCAGCTTCCGGCGCAACGCGCATCTGCGGCGGCACCTGGCTACCCATGCGGAGCCCGGG CAGGAGCAGGCCGAGCCCCCGCAGGAGTGCGTGGAGTGCGGGAAGAGCTTCAGCCGCAGCTGCAATCTG CTGCGACACCTGCTGGTGCACACGGGCGCCAGGCCCTACTCCTGCACGCAGTGTGGCCGCAGCTTCAGC CGCAACTCCCACCTGCTGCGCCACCTGCGCACCCACGCCCGCGAGACGCTGTACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282415
Insert Size	1161 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282415.1
RefSeq Size	2281 bp
RefSeq ORF	1161 bp
Locus ID	84891
UniProt ID	Q96SZ4
Protein Families	ES Cell Differentiation/IPS, Transcription Factors
MW	43.9 kDa
Gene Summary	Embryonic stem (ES) cell-specific transcription factor required to maintain ES cell pluripotency. Can both activate and /or repress expression of target genes, depending on the context. Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence. Regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate 5' structure which results in the use of a downstream in-frame AUG, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.