Myotilin (MYOT) (NM_001300911) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LGMD1; LGMD1A; MFM3; TTID; TTOD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335764 representing NM_001300911. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATTCCAACTATCAACAGTCCTCAGCTGGCCAACCTATAAATGCAAAGCCATCCCAAACTGCAAAT GCTAAGCCCATACCAAGAACTCCTGATCATGAAATACAAGGATCAAAAGAAGCTTTGATTCAAGATTTG GAAAGAAAGCTGAAATGCAAGGACACCCTTCTTCATAATGGAAATCAACGTCTAACATATGAAGAGAAG ATGGCTCGCAGATTGCTAGGACCACAGAATGCAGCTGCTGTGTTTCAAGCTCAGGATGACAGTGGTGCA CAAGACTCGCAGCAACACAACTCAGAACATGCGCGACTGCAAGTTCCTACATCACAAGTAAGAAGTAGA TCAACCTCAAGGGGAGATGTGAATGATCAGGATGCAATCCAGGAGAAATTTTACCCACCACGTTTCATT CAAGTGCCAGAGAACATGTCGATTGATGAAGGAAGATTCTGCAGAATGGACTTCAAAGTGAGTGGACTG CCAGCTCCTGATGTGTCATGGTATCTAAATGGAAGAACAGTTCAATCAGATGATTTGCACAAAATGATA GTGTCTGAGAAGGGTCTTCATTCACTCATCTTTGAAGTAGTCAGAGCTTCAGATGCAGGGGCTTATGCA TGTGTTGCCAAGAATAGAGCAGGAGAAGCCACCTTCACTGTGCAGCTGGATGTCCTTGCAAAAGAACAT AAAAGAGCACCAATGTTTATCTACAAACCACAGAGCAAAAAAGTTTTAGAGGGAGATTCAGTGAAACTA GAATGCCAGATCTCGGCTATACCTCCACCAAAGCTTTTCTGGAAAAGAAATAATGAAATGGTACAATTC AACACTGACCGAATAAGCTTATATCAAGATAACACTGGAAGAGTTACTTTACTGATAAAAGATGTAAAC AAGAAAGATGCTGGGTGGTATACTGTGTCAGCAGTTAATGAAGCTGGAGTGACTACATGTAACACAAGA TTAGACGTTACGGCACGTCCAAACCAAACTCTTCCAGCTCCTAAGCAGTTACGGGTTCGACCAACATTC AGCAAATATTTAGCACTTAATGGGAAAGGTTTGAATGTAAAACAAGCTTTTAACCCAGAAGGAGAATTT CAGCGTTTGGCAGCTCAATCTGGACTCTATGAAAGTGAAGAACTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300911
Insert Size	1152 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300911.1
RefSeq Size	1939 bp
RefSeq ORF	1152 bp
Locus ID	9499
UniProt ID	Q9UBF9
MW	42.9 kDa
Gene Summary	This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008] Transcript Variant: This variant (3) lacks an internal segment in the 5' region, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (c) has a shorter N-terminus, compared to isoform a.

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