EST1A (SMG6) (NM_001282326) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C17orf31; EST1A; hSMG5/7a; SMG-6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335386 representing NM_001282326. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGATTTAGGCTTTGCCACCAGAGGGGTTGCTGTCCACATGAGAGAGAGAACACCTGCACCTGTAAA ATGATCATCAGTAGTCTCCAGGTTATTGCAGCTGACTGCAAAAGGGTCACAGTGCTGAAGTATTTTCTG GAAGCCCTTTGTGGACAAGAAGAGCCTCTGCTGGCATTCAAGGGTGGAAAGTATGTGTCAGTGGCACCC GTCCCAGACACCATGGGAAAGGAAATGGGAAGCCAAGAGGGAACACGACTGGAAGATGAGGAGGAGGAT GTGGTGATTGAAGACTTTGAGGAAGATTCAGAGGCTGAAGGCAGCGGAGGCGAGGATGACATCAGGGAG CTTCGGGCCAAGAAGCTGGCTCTGGCCAGGAAGATAGCTGAGCAGCAGCGTCGCCAGGAAAAGATCCAG GCTGTCCTGGAGGACCACAGTCAGATGAGGCAGATGGAGCTCGAAATCAGACCTTTGTTCCTCGTACCA GACACCAACGGCTTCATTGACCACCTGGCCAGTCTGGCGCGGCTGCTGGAGAGCAGGAAGTACATCCTG GTGGTGCCCCTCATCGTGATCAATGAGCTGGACGGCCTGGCCAAGGGGCAGGAGACAGACCACCGGGCT GGGGGCTACGCCCGTGTGGTACAAGAGAAGGCCCGCAAGTCCATCGAGTTCCTCGAGCAGCGATTCGAG AGTCGGGACTCTTGCCTGCGAGCCCTGACCAGCCGTGGCAATGAACTCGAATCCATCGCCTTCCGCAGT GAGGACATCACTGGCCAGCTGGGTAACAACGATGATCTCATCCTGTCCTGCTGCCTCCACTACTGCAAA GACAAGGCTAAGGACTTCATGCCCGCCAGCAAAGAGGAGCCAATCCGGCTACTGCGGGAGGTGGTGCTG TTGACGGATGACCGGAACCTGCGTGTGAAGGCGCTCACAAGGAATGTTCCTGTACGGGACATCCCAGCC TTCCTCACGTGGGCCCAGGTGGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282326
Insert Size	993 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282326.1
RefSeq Size	4243 bp
RefSeq ORF	993 bp
Locus ID	23293
UniProt ID	Q86US8
MW	37.5 kDa
Gene Summary	This gene encodes a component of the telomerase ribonucleoprotein complex responsible for the replication and maintenance of chromosome ends. The encoded protein also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD. Alternatively spliced transcript variants encoding distinct protein isoforms have been described. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (5) uses an alternate internal promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at an alternate AUG, and the resulting isoform (4) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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