Nup53 (NUP35) (NM_001287584) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MP-44; MP44; NP44; NUP53
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335251 representing NM_001287584. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGCTGGGTTCACCCACATCTCCAAAGCCAGGAGTTAATGCCCAGTTCTTACCTGGATTTTTAATG GGGGATTTGCCAGCTCCGGTGACTCCACAACCTCGATCAATTAGTGGCCCTTCAGTAGGAGTAATGGAA ATGAGATCACCTTTACTTGCAGGTGGGTCACCACCACAACCAGTTGTACCAGCTCATAAAGATAAAAGT GGCGCTCCACCAGTTAGAAGTATATATGATGACATTTCTAGCCCAGGACTTGGATCAACACCTTTAACT TCAAGAAGACAGCCAAACATTTCAGTAATGCAGAGTCCTCTTGTTGGAGTTACATCTACTCCTGGAACA GGGCAAAGTATGTTTAGTCCAGCAAGTATCGGTCAGCCACGAAAGACGACATTATCTCCTGCCCAGTTG GATCCTTTTTATACTCAAGGAGATTCTTTGACTTCAGAAGATCACCTCGATGACTCTTGGGTGACTGTA TTTGGGTTTCCTCAAGCATCTGCTTCCTACATATTACTACAATTTGCACAGTATGGGAATATCTTAAAA CATGTGATGTCTAATACAGGAAATTGGATGCATATTCGTTATCAATCTAAACTGCAGGCTCGGAAAGCC TTAAGCAAAGATGGGAGGATTTTTGGAGAATCCATCATGATTGGTGTAAAACCATGTATTGACAAAAGT GTTATGGAAAGCAGTGACAGATGTGCTTTATCATCTCCATCTTTAGCCTTTACACCACCAATCAAAACT CTAGGTACACCAACACAACCTGGAAGTACTCCTAGGATTTCTACCATGAGACCTCTTGCTACAGCATAC AAAGCCTCTACTAGTGATTATCAGGTTATTTCTGACAGACAAACGCCAAAAAAAGATGAAAGTCTTGTA TCCAAAGCAATGGAGTACATGTTTGGCTGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287584
Insert Size	930 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287584.1
RefSeq Size	1740 bp
RefSeq ORF	930 bp
Locus ID	129401
UniProt ID	Q8NFH5
MW	33.1 kDa
Gene Summary	This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (3) differs in its 5' UTR, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.

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