PRR16 (NM_001300783) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DSC54; LARGEN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335221 representing NM_001300783. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCAGCCAAGTCCAAGGGGAACCCCTCCTCGTCCTGTCCAGCCGAGGGACCGCCGGCAGCCTCCAAA ACCAAGGTGAAGGAACAGATCAAGATCATCGTGGAGGATTTGGAATTAGTCCTGGGCGACCTGAAGGAC GTGGCCAAGGAACTTAAGGAGGTGGTTGACCAGATTGACACCCTGACCTCTGACCTACAGCTGGAGGAT GAGATGACTGACAGCTCCAAAACGGACACGCTGAATAGTAGCTCAAGTGGCACAACAGCCTCCAGCCTA GAGAAGATCAAAGTGCAGGCTAATGCACCGCTTATTAAACCCCCAGCACACCCATCTGCTATCCTCACG GTCCTGAGAAAGCCAAACCCTCCACCACCTCCTCCAAGGTTGACACCTGTGAAGTGTGAAGACCCCAAA AGGGTGGTTCCAACTGCCAATCCTGTAAAAACCAATGGCACCCTTCTACGAAATGGAGGCTTACCAGGT GGACCTAACAAAATTCCAAATGGAGATATCTGCTGCATACCCAACAGTAACTTGGACAAGGCTCCAGTC CAGCTTCTGATGCATAGACCTGAAAAAGACAGATGTCCCCAGGCAGGGCCTCGAGAACGAGTTCGGTTT AATGAAAAAGTACAGTACCATGGCTATTGTCCTGACTGTGATACCCGGTATAACATAAAAAACAGGGAG GTCCACTTACACAGTGAACCTGTCCACCCACCGGGAAAGATTCCTCACCAAGGCCCTCCCCTCCCTCCT ACACCCCATCTCCCTCCTTTCCCACTAGAAAATGGGGGAATGGGAATAAGCCACAGTAACAGCTTCCCC CCTATCAGACCTGCAACTGTGCCTCCTCCCACTGCACCAAAACCACAGAAGACGATCTTGAGGAAGTCA ACCACTACAACCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300783
Insert Size	915 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300783.1
RefSeq Size	1754 bp
RefSeq ORF	915 bp
Locus ID	51334
UniProt ID	Q569H4
MW	32.8 kDa
Gene Summary	Regulator of cell size that promotes cell size increase independently of mTOR and Hippo signaling pathways. Acts by stimulating the translation of specific mRNAs, including those encoding proteins affecting mitochondrial functions. Increases mitochondrial mass and respiration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.