INSYN1 (NM_001303254) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C15orf59
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001303254, the custom clone sequence may differ by one or more nucleotides ATGAAGATGGTCATCGGGCAGCTTGAGGGCATCCTTCGCGAGCTCAAGGAGGTGGCCAAGGAGCTGAGGG AGGTGGTGAGCCAGATCGATAAGCTAACCTCGGACTTCGACTTTGAACTGGAGCCGGACGACTGGACCAC GGCCACTGTGAGCAGCACCTCTAGCAGTGACAAGGCGGGCATGGGTGGCCCCTTTGACCTGGGCCACCTG GACTTCATGACAGCCGATATCCTCTCAGACAGCTGGGAGTTCTGCTCCTTCCTGGACGTCTCTACCCCCT CGGACTCCGTGGATGGTCCCGAGTCGACTCGGCCAGGGGCTGGCCCTGACTACCGCCTCATGAATGGTGG CACGCCCATCCCCAATGGGCCACGAGTGGAGACCCCAGACTCCTCCAGTGAGGAGGCCTTTGGTGCTGGC CCCACGGTGAAGAGCCAGCTGCCCCAGCGGACCCCAGGGACACGGGAGAGGGTGCGGTTCAGTGACAAAG TGCTCTACCATGCTCTGTGCTGTGACGATGAGGAGGGGGACGGTGAGCAGGAGGTGGAGGAGGAAGAAGT GGGCTTGCCCCCTGAGCCTGCCCATACAGAGGCCCATGCAGGCCCCCACAAGCCCTCCCCAGCCCCCTAC AAGTCACGGCGCTCTCCACTGACCAGCCGCCACTCAGGCTCTACCTTGGCCCCTGAACAGACTCGAAGGG TCACGAGGAACAGCAGCACCCAGACAGTGTCAGACAAGAGCACGCAGACGGTTCTGCCCTACACAGCCAC TAGACAGAAAGCCAGGGGGAAAAACTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001303254
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303254.1, NP_001290183.1
RefSeq Size	5613 bp
RefSeq ORF	798 bp
Locus ID	388135
Gene Summary	Component of the protein machinery at the inhibitory synapses, probably acting as a scaffold. Inhibitory synapses dampen neuronal activity through postsynaptic hyperpolarization. This synaptic inhibition is fundamental for the functioning of the central nervous system, shaping and orchestrating the flow of information through neuronal networks to generate a precise neural code.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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