RNA terminal phosphate cyclase like 1 (RCL1) (NM_001286699) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RNAC; RPCL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286699, the custom clone sequence may differ by one or more nucleotides ATGCCTCCCGGAGGAGGAGGCGAAGTGGTTTTCTCATGTCCTGTGAGGAAGGTCTTGAAGCCCATTCAAC TCACAGATCCAGGAAAAATCAAACGTATTAGAGGAATGGCGTACTCTGTACGTGTGTCACCTCAGATGGC GAACCGGATTGTGGATTCTGCAAGGAGCATCCTCAACAAGTTCATACCTGATATCTATATTTACACAGAT CACATGAAAGGAGTCAACTCTGGGAAGTCTCCGGGCTTTGGGTTGTCACTGGTTGCTGAGACCACCAGTG GCACCTTCCTCAGTGCTGAACTGGCCTCCAACCCCCAGGGCCAGGGAGCAGCAGTACTTCCAGAGGACCT TGGCAGGAACTGTGCCCGGCTGCTGCTGGAGGAAATCTACAGGGGTGGATGCGTAGACTCGACCAACCAA AGCCTGGCGCTACTACTCATGACCCTTGGACAGCAGGATGTTTCCAAAGTCCTGCTAGGCCCTCTCTCTC CCTACACGATAGAATTTTTGCGGCATTTGAAGAGCTTTTTCCAGATTATGTTTAAAATTGAAACCAAGCC ATGTGGTGAAGAACTCAAGGGTGGGGATAAAGTGCTGATGACCTGTGTTGGCATTGGTTTCTCCAACCTT AGCAAGACCCTCAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286699
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286699.1, NP_001273628.1
RefSeq Size	1950 bp
RefSeq ORF	648 bp
Locus ID	10171
UniProt ID	Q9Y2P8
Gene Summary	Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two alternate exons in the 5' region and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Both variants 2 and 3 encode isoform b.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.