CCDC53 (WASHC3) (NM_001301107) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCDC53; CGI-116
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301107, the custom clone sequence may differ by one or more nucleotides ATGGATGAGGACGGGCTTCCTCTCATGGGGTCAGGCATAGACCTGACCAAGGTGCCAGCTATTCAACAGA AAAGAACGGTGGCTTTTCTAAACCAATTTGTGGTGCACACTGTACAGTTCCTCAACCGCTTTTCTACAGT TTGTGAGGAGAAACTGGCAGACCTTTCACTTCGTATCCAACAAATTGAAACAACTCTCAATATTTTAGAT GCAAAGTTGTCATCTATCCCAGGCCTAGATGATGTCACAGTTGAAGTATCTCCTTTAAATGTCACCAGTG TCACAAATGGAGCACATCCTGAAGCCACTTCAGAGCAACCACAGAACAGTACACAAGACTCTGGACTACA GGAAAGTGAAGTATCAGCAGAAAATATCTTAACTGTAGCCAAGGATCCAAGATATGCCAGATATCTCAAA ATGGTTCAAGTGGGTGTACCAGTGATGGCAATAAGAAACAAAATGATATCAGAAGGACTAGACCCAGATC TTCTTGAGAGGCCAGATGCTCCAGTGCCTGATGGCGAAAGTGAGAAAACTGTAGAAGAAAGTTCAGATAG CGAATCTTCTTTTAGTGATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301107
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301107.1, NP_001288036.1
RefSeq Size	1012 bp
RefSeq ORF	582 bp
Locus ID	51019
Gene Summary	Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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