NEURL3 (NM_001285486) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LINCR; RNF132
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC333763 representing NM_001285486. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACGTGTATGGGACCACTAAGGCCATCGAGCTGCTGGATCCCACAGCCAGCCGGCTCCCAACACCC ATGCCATGGGACCTCAGCAACAAGGCTGTGCCTGAGCCCAAAGGCTTTGGGCCAAAAGATTTGGCTCTG CCTACTGGAAACCTAAAAGCAACCTGGCATCTCTGCCTTCGAGAGCATTTGCCTACTCTCCACACCAGG AGAGGAGTGTGCCATCTGCTTCTATCACGCTGCCAACACCCGCCTTGTGCCCTGCGGCCACACATACTT CTGCAGATACTGTGCCTGGCGGGTCTTCAGCGATACGGCCAAGTGCCCTGTGTGCCGCTGGCAGATAGA GGCGGTAGCCCCTGCGCAGGGCCCTCCTGCTCTGAGGGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001285486
Insert Size	387 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285486.1
RefSeq Size	1770 bp
RefSeq ORF	387 bp
Locus ID	93082
MW	13.9 kDa
Gene Summary	E3 ubiquitin-protein ligase. Seems to utilize UBE2E1. In vitro, generates polyubiquitin chains via non-canonical lysine residues suggesting that it is not involved in tagging substrates for proteasomal degradation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains two alternate exons, one of which results in a frameshift, compared to variant 1. The resulting protein (isoform b) uses a downstream, in-frame AUG and has a distinct C-terminus, compared to isoform a.

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