Uroplakin IIIB (UPK3B) (NM_182683) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	P35; UP3B; UPIIIB
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333309 representing NM_182683. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGCTACCCTGGGGGCAGCCTCACCTAGGGCTGCAGATGCTCCTCCTGGCGTTGAACTGTCTCCGG CCCAGCCTGAGCCTGGAGCTGGTGCCCTACACACCACAGATAACAGCTTGGGACCTGGAAGGGAAGGTC ACAGCCACCACCTTCTCCCTGGAGCAGCCGCGCTGTGTCTTCGATGGGCTTGCCAGCGCCAGCGATACC GTCTGGCTCGTGGTGGCCTTCAGCAATGCCTCCAGGGGCTTCCAGAACCCGGAGACACTGGCTGACATT CCGGCCTCCCCACAGCTGCTGACCGATGGCCACTACATGACGCTGCCCCTGTCTCCGGACCAGCTGCCC TGTGGCGACCCCATGGCGGGCAGCGGAGGCGCCCCCGTGCTGCGGGTGGGCCATGACCACGGCTGCCAC CAGCAGCCCTTCTGCAACGCGCCCCTCCCTGGCCCTGGACCCTATCGGGAAGACCCCCGGATCCATCGA CACCTGGCCAGGGCGGCGAAGTGGCAGCATGATCGTCATTACCTCCATCCTCTCTTCTCTGGCCGGCCT CCTACTCTTGGCCTTCTTGGCAGCCTCTACCATGCGCTTCTCCAGCCTGTGGTGGCCGGAGGAGGCCCC GGAGCAGCTGCGGATCGGCTCCTTCATGGGCAAGCGCTACATGACCCACCACATCCCACCCAGCGAGGC CGCCACACTGCCGGTGGGCTGCAAGCCTGGCCTGGACCCCCTCCCCAGCCTCAGCCCCTAGCCTGGCCT CTTTGCATGGGGCTGGGGGAGATGGGGCGCCGGGAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_182683
Insert Size	798 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_182683.1
RefSeq Size	1460 bp
RefSeq ORF	798 bp
Locus ID	105375355
UniProt ID	Q9BT76
MW	28.5 kDa
Gene Summary	UPK3B is a minor component of the apical plaques of mammalian urothelium that binds and dimerizes with uroplakin-1b (UPK1B; MIM 602380), one of the major conserved urothelium membrane proteins. The other major conserved integral membrane proteins of urothelial plaques are UPK1A (MIM 611557), UPK2 (MIM 611558), and UPK3A (MIM 611559) (Deng et al., 2002 [PubMed 12446744]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (3) is alternatively spliced at the 5' end compared to variant 1, which results in a shorter isoform (c) lacking a protein segment in the N-terminal region compared to isoform a. This variant is annotated on the ALT_REF_LOCI_1 alternate haplotype of the GRCh38 human reference genome assembly.

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