BBX (NM_001276286) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARTC1; HBP2; HSPC339; MDS001
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333235 representing NM_001276286. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAAGGCAGTAATAGAAATAAGGATCATTCAGCAGAAGGAGAAGGGGTTGGAAAACGACCAAAACGA AAGTGTCTTCAGTGGCATCCATTGCTAGCAAAGAAACTTCTTGATTTTTCAGAAGAGGAAGAAGAGGAA GACGAAGAGGAGGATATTGATAAGGTTCAACTTCTTGGGGCCGATGGCCTAGAGCAAGATGTTGGTGAA ACTGAAGATGATGAATCACCAGAGCAGCGAGCCCGGAGACCAATGAATGCATTTCTTTTATTTTGCAAA CGCCATCGCTCTCTTGTACGTCAGGAACACCCCAGGCTTGATAACCGAGGTGCTACCAAGATACTAGCT GATTGGTGGGCTGTTCTTGATCCAAAGGAAAAGCAGAAATACACAGACATGGCCAAGGAGTATAAGGAT GCATTTATGAAAGCAAATCCTGGCTACAAATGGTGTCCTACCACAAACAAGCCTGTGAAATCCCCAACA CCCACTGTCAATCCACGAAAGAAACTTTGGGCCTTCCCATCTGACTCTTCAAGAGACTTGCCAAGCCCC AAGAAAGCAAAGACTGAAGAAATGCCTCAGCTTAACTTTGGAATGGCTGATCCTACTCAAATGGGAGGC CTGAGTATGCTGCTGTTAGCTGGAGAACATGCTCTTGGCACACCAGAGGTATCCTCTGGCACATGCAGG CCTGATGTTTCAGAATCTCCTGAATTACGTCAGAAGTCACCATTGTTTCAGTTTGCCGAGATATCTTCA AGTACGTCCCACTCTGATGCTTCTACAAAGCAGTGTCAAACATCTGCCTTGTTTCAGTTTGCAGAGATT TCTTCAAACACTTCGCAGTTGGGTGGTGCTGAGCCTGTAAAACGCTGTGGAAAGTCTGCACTCTTTCAA CTGGCAGAGGAAAACGAAGCTCAGGAAAAGGAAACTCCTCTGATCATGAAGGGTGTTGGAATGAAGAAA GCTGGACATTTAGTCAGAGTGGGACCAGTGGGAGCAAGAAGTTCAAGAAGACAAAGCCAAAAGAAGACT GTCTCCTTGGCTCCGCAAAGCTGGATGAAGAATTTGAAAAAAAATTCAACAGCCTCCCTCAATATAGTC CTGTTACATTTGACCGGAAATGTGTACCTGTCCCAAGAAAAAAGAAGAAGACTGGAAATGTGTCCTCAG AACCGACTAAAACCAGCAAAGGTCCTTTCCAGTCTCAGAAAAAGAACTTATTCCACAAAATTGTCAGCA AATATAAGCACAAAAAGGAGAAGCCCAATGTTCCGGAAAAAGGAAGTGGGGATAAATGGTCAAACAAGC AACTCTTCTTGGATGCCATTCACCCTACAGAAGCCATATTTTCAGAAGACAGAAACACCATGGAGCCTG TTCATAAGGTTAAAAATATCCCATCCATTTTCAACACTCCAGAGCCAACAACAACGCAAGAACCTTTGG TGGGCAGCCAAAAGAGAAAAGCAAGGAAAACCAAGATTACACACCTTGTCAGGACAGCAGATGGCCGGG TATCACCAGCAGGAGGTACTTTGGATGACAAACCAAAGGAACAACTGCAGAGGAGTCTCCCTAAAGCAA CTGAGACAGACTGCAATGACAAATGCTCACACAACACCGAGGTCGGGGAGACGCGGAGCAGTACTCCAG AAATGCCTGCCGTGTCTGCGTTCTTTAGCCTCGCTGCGCTGGCTGAAGTGGCAGCCATGGAAAATGTGC ACAGAGGTCAGAGGTCAACTCCGCTCACCCATGATGGACAGCCAAAAGAAATGCCGCAGGCTCCTGTAC TTATTTCCTGCGCTGACCAGTGAAGCGCCCTTTCATTGTAAAACATTGTGCTTTACCTACTACCCTAGC CTTGTCTTTACCGAGGGATGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001276286
Insert Size	1887 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276286.1
RefSeq Size	8028 bp
RefSeq ORF	1887 bp
Locus ID	56987
UniProt ID	Q8WY36
Protein Families	Transcription Factors
MW	71.5 kDa
Gene Summary	Transcription factor that is necessary for cell cycle progression from G1 to S phase.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate exon in the coding region, which results in a frameshift, compared to variant 1. The resulting isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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