CEP164 (NM_001271933) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NPHP15
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333156 representing NM_001271933. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTGGACGACCCCTCCGCATAGGAGATCAGCTGGTTCTGGAAGAAGATTATGATGAGACCTACATT CCTAGTGAGCAAGAAATTCTTGAATTTGCCCGGGAGATTGGTATTGATCCCATCAAGGAACCAGAACTG ATGTGGCTGGCGCGAGAGGGCATCGTGGCCCCACTGCCTGGAGAGTGGAAACCATGCCAGGACATCACA GGTGACATTTACTATTTCAACTTCGCCAACGGGCAGTCTATGTGGGACCATCCATGTGACGAACACTAT CGGAGCTTGGTGATCCAAGAGCGGGCAAAGCTGTCAACTTCTGGGGCCATTAAGAAGAAGAAAAAAAAA AAGGAAAAGAAAGACAAGAAGGACAGAGACCCCCCCAAAAGTTCGCTGGCCTTGGGTTCCTCATTAGCC CCAGTTCATGTTCCTCTTGGGGGCCTGGCTCCTTTACGAGGTCTTGTGGATACCCCACCCTCTGCTCTT CGTGGATCTCAAAGCGTGAGCCTGGGGAGCTCAGTGGAGTCTGGACGTCAGCTTGGAGAACTCATGCTG CCTTCACAGGGTCTCAAGACCTCTGCTTATACAAAGGGTCTCTTGGGCTCCATATATGAGGACAAGACT GCTCTCAGCCTCTTGGGTTTAGGAGAAGAAACCAATGAGGAGGATGAGGAGGAAAGTGACAACCAGAGT GTCCACAGCTCAAGTGAGCCTCTTAGGAACCTACACCTGGACATTGGGGCACTGGGGGGTGACTTTGAG TATGAGGAGTCTCTGAGAACAAGCCAGCCAGAGGAGAAGAAGGATGTTTCTCTGGATTCAGATGCTGCC GGTCCCCCTACTCCCTGCAAGCCCTCCAGCCCAGGTGCAGACAGCAGTCTGAGCAGTGCTGTTGGCAAA GGGCGACAGGGAAGTGGAGCAAGACCTGGTCTTCCAGAAAAAGAGGAAAATGAGAAGAGTGAACCTAAG ATTTGCAGGAATCTGGTGACCCCCAAGGCAGACCCTACAGGCAGTGAGCCTGCCAAAGCCTCTGAAAAG GAAGCACCAGAGGACACAGTAGATGCAGGAGAGGAGGGTTCCAGGAGGGAAGAGGCAGCCAAGGAGCCA AAGAAGAAGGCTTCTGCTCTGGAAGAGGGCAGTTCAGACGCCAGCCAAGAACTGGAAATTAGTGAACAC ATGAAGGAACCACAGCTCTCAGACTCCATAGCTTCTGACCCCAAGTCCTTCCATGGCCTGGACTTCGGT TTTCGCAGCCGGATCTCGGAGCACCTGCTGGATGTTGATGTGCTTTCCCCAGTCCTGGGTGGAGCTTGT CGGCAGGCCCAGCAACCACTGGGAATAGAAGACAAGGATGACAGCCAGTCCAGCCAAGATGAGCTGCAG AGCAAGCAGTCCAAAGGCCTGGAGGAGAGGTACCATAGGTTATCTCCTCCACTTCCACACGAGGAGCGG GCCCAGAGTCCCCCTCGCAGCCTGGCCACTGAAGAAGAGCCTCCCCAGGGCCCCGAGGGGCAGCCCGAG TGGAAGGAGGCAGAGGAGCTTGGGGAGGACTCTGCAGCCAGCCTCAGCCTGCAGCTGTCCCTCCAGAGG GAGCAGGCCCCAAGCCCACCTGCTGCCTGTGAGAAGGGCAAGGAGCAGCATTCCCAGGCCGAGGAGCTG GGCCCTGGGCAGGAAGAGGCAGAGGATCCTGAGGAGAAGGTGGCGGTCAGCCCCACCCCGCCAGTCTCT CCAGAGGTGCGATCCACAGAGCCTGTGGCTCCCCCAGAGCAGCTCTCAGAGGCTGCACTAAAGGCCATG GAAGAGGCAGTGGCCCAAGTACTCGAGCAAGACCAGAGGCACCTGCTGGAATCCAAGCAAGAGAAGATG CAGCAACTGCGGGAGAAGCTGTGCCAAGAGGAGGAAGAGGAGATCCTCCGGCTTCACCAGCAGAAAGAG CAATCTCTCAGTTCCTTGAGGGAGCGGCTGCAGAAAGCCATTGAGGAGGAGGAGGCCCGGATGAGAGAG GAGGAAAGCCAGAGGCTATCCTGGCTCCGAGCTCAGGTCCAGTCCAGCACACAAGCAGATGAGGACCAA ATCAGGGCTGAGCAAGAGGCTTCCCTGCAGAAACTGAGAGAAGAGTTGGAGTCTCAACAGAAGGCTGAG AGGGCCAGCTTGGAACAGAAAAATAGGCAAATGCTGGAGCAGCTCAAGGAAGAGATAGAGGCTTCGGAG AAGAGCGAGCAGGCTGCCCTGAATGCTGCAAAGGAGAAGGCTCTGCAGCAGCTGAGGGAGCAGCTGGAA GGGGAGAGGAAAGAAGCTGTGGCAACGCTGGAGAAGGAGCACAGTGCTGAGCTGGAGCGGCTCTGCTCC TCATTGGAGGCCAAGCACCGGGAGGTGGTCTCCAGCCTCCAGAAGAAGATACAGGAAGCTCAACAGAAA GAGGAGGCCCAGCTGCAGAAGTGCCTTGGGCAAGTGGAGCACAGAGTTCACCAGAAGTCTTATCACGTG GCTGGGTATGAGCACGAGCTCAGCAGTCTCCTGCGAGAGAAGCGCCAGGAAGTGGAAGGGGAGCATGAG AGGAGGTTGGACAAGATGAAGGAGGAGCACCAGCAAGTGATGGCTAAGGCCAGAGAGCAGTATGAAGCT GAGGAGAGGAAGCAGCGGGCTGAGCTTCTGGGGCACCTGACCGGAGAGCTGGAGCGCCTGCAGAGGGCC CATGAACGAGAACTGGAGACTGTGAGGCAGGAGCAACACAAGCGTCTTGAGGACTTGCGGCGCCGGCAC AGGGAGCAGGAAAGGAAGCTCCAGGATTTAGAGTTGGACCTTGAAACCAGAGCTAAAGATGTCAAGGCC AGATTGGCTCTGCTGGAGGTCCAGGAGGAGACCGCCCGGAGGGAGAAGCAGCAGCTGCTTGATGTGCAG AGGCAGGTTGCTCTGAAGAGTGAGGAAGCCACAGCCACCCATCAGCAGCTGGAGGAGGCACAGAAGGAG CACACCCACCTGTTGCAGTCAAACCAGCAGCTCCGAGAAATTCTTGATGAGCTGCAGGCCCGCAAGCTG AAGCTGGAGTCCCAAGTGGATCTGCTGCAGGCTCAGAGCCAGCAACTGCAGAAACACTTCAGCAGCCTG GAGGCTGAAGCTCAAAAGAAGCAGCACCTGTTGAGAGAAGTGACAGTTGAGGAAAATAATGCTTCCCCA CATTTTGAGCCAGATCTCCATATTGAGGACCTGAGGAAATCCCTTGGAACAAACCAGACCAAAGAGGTG TCTTCTTCTCTCTCCCAGAGCAAGGAGGACTTATACTTGGACAGCCTGTCCTCCCACAATGTCTGGCAC CTCCTCTCTGCTGAGGGGGTAGCCCTCCGTAGTGCCAAGGAGTTCCTTGTGCAGCAGACACGCTCCATG CGGAGGCGGCAGACAGCTCTGAAAGCTGCCCAGCAGCATTGGCGCCATGAGCTGGCCAGTGCGCAGGAG GTGGCCAAAGACCCACCAGGCATCAAGGCCCTGGAAGATATGCGCAAGAACCTGGAGAAGGAGACCAGG CACCTGGATGAGATGAAGTCGGCCATGCGGAAAGGCCACAACCTGCTGAAGAAGAAAGAGGAGAAGCTG AATCAGTTGGAGTCCTCTCTTTGGGAAGAGGCCTCAGATGAGGGCACTCTGGGAGGATCCCCCACCAAG AAGGCAGTAACCTTCGACCTCAGTGACATGGACAGCCTGAGCAGTGAAAGTTCTGAATCTTTTTCCCCG CCTCACCTCGACTCAACCCCGAGTCTCACCTCCCGCAAGATCCACGGGCTTAGCCACTCCCTCCGGCAG ATCAGCAGCCAGCTGAGCAGTGTCCTCAGCATCCTGGACAGCCTCAACCCTCAGTCGCCGCCGCCGCTC CTCGCCTCCATGCCAGCCCAGCTCCCTCCCCGGGACCCTAAGAGCACCCCCACCCCCACCTACTATGGC TCCCTGGCCAGGTTCTCAGCCTTATCATCTGCTACACCCACGTCCACCCAATGGGCCTGGGATTCAGGG CAGGGGCCCAGGCTCCCCTCCTCTGTGGCTCAAACGGTGGACGACTTCCTGTTGGAGAAGTGGCGCAAG TATTTTCCATCTGGCATCCCGCTGCTCAGCAACAGCCCCACCCCGCTGGAGAGCAGGCTGGGTTACATG TCTGCCAGTGAGCAGCTCCGGCTCCTACAGCACTCCCATTCGCAAGTCCCTGAGGCGGGCAGCACCACC TTTCAGGGCATAATTGAGGCCAACCGGAGGTGGCTGGAACGTGTCAAGAATGACCCCAGGTTACCTCTC TTCTCGTCAACACCCAAGCCAAAAGCTACTTTGAGCCTCCTGCAGCTGGGCCTTGATGAGCACAACAGA GTGAAGGTGTATCGCTTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271933
Insert Size	4368 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271933.1
RefSeq Size	5550 bp
RefSeq ORF	4368 bp
Locus ID	22897
UniProt ID	Q9UPV0
MW	163.5 kDa
Gene Summary	This gene encodes a centrosomal protein involved in microtubule organization, DNA damage response, and chromosome segregation. The encoded protein is required for assembly of primary cilia and localizes to mature centrioles. Defects in this gene are a cause of nephronophthisis-related ciliopathies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) differs in the 5' UTR, uses an alternate in-frame splice site at the 3' end of an exon, and uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (2) contains an alternate internal segment and lacks another alternate internal segment compared to isoform 1.

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