PSD (NM_001270965) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EFA6; EFA6A; PSD1; TYL
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333015 representing NM_001270965. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCAGGGTGCCATGCGCTTCTGCTCGGAAGGCGACTGTGCCATCTCCCCACCACGATGCCCACGC CGCTGGCTCCCCGAAGGCCCGGTGCCCCAGAGCCCCCCAGCCAGCATGTATGGCAGCACAGGCTCCTTG CTACGGCGAGTGGCAGGTCCAGGTCCTCGAGGCCGGGAACTGGGACGTGTGACAGCACCCTGTACACCT CTGCGTGGCCCCCCCTCACCCCGTGTTGCTCCCTCACCCTGGGCACCCTCTTCACCCACTGGGCAGCCC CCACCCGGGGCCCAGAGCTCTGTGGTCATCTTCCGCTTTGTGGAGAAGGCCAGTGTGAGGCCACTGAAT GGGCTACCTGCTCCAGGGGGCTTGAGTCGGAGCTGGGATCTGGGTGGGGTTTCTCCTCCCAGGCCCACC CCAGCCCTTGGGCCTGGCTCCAACCGGAAGTTACGGCTGGAAGCATCCACATCAGACCCACTCCCCGCC AGAGGAGGCTCGGCCCTACCTGGCAGCCGGAACCTTGTACATGGGCCGCCAGCCCCACCCCAGGTTGGA GCAGATGGCCTTTACTCCTCTCTCCCCAATGGGCTGGGGGGCCCCCCTGAGCGCCTGGCCACACTCTTC GGAGGACCTGCTGACACTGGATTCCTGAACCAGGGGGATACCTGGTCCTCCCCCCGGGAAGTCTCCTCT CATGCCCAGAGAATCGCTAGAGCCAAATGGGAATTCTTCTATGGCTCCTTGGACCCCCCCAGCTCAGGT GCTAAGCCCCCAGAGCAGGCCCCCCCATCTCCACCTGGGGTGGGCTCAAGGCAGGGCTCTGGGGTGGCT GTGGGGCGAGCAGCCAAGTACTCCGAGACAGACCTGGACACGGTGCCCCTGAGGTGCTACCGCGAGACT GACATCGATGAGGTGCTGGCTGAGCGGGAGGAGGCCGACTCGGCCATCGAAAGTCAGCCCAGCTCTGAG GGCCCACCAGGCACTGCCTACCCACCTGCCCCACGGCCCGGCCCACTCCCTGGCCCTCATCCCAGCCTC GGCAGTGGCAATGAGGATGAGGACGACGATGAGGCAGGTGGGGAAGAAGATGTGGACGACGAGGTGTTT GAGGCCTCTGAAGGGGCCCGGCCAGGGAGCCGGATGCCTCTCAAGTCACCTGTGCCCTTTCTACCTGGG ACGAGCCCCTCGGCTGATGGGCCTGACTCTTTCAGTTGTGTGTTCGAAGCCATCCTGGAGTCACACCGG GCCAAAGGCACCTCCTATACCAGCCTCGCCTCGCTGGAGGCCTTGGCCTCACCTGGCCCAACCCAGAGC CCCTTCTTCACCTTTGAGCTGCCTCCCCAACCCCCTGCACCCCGGCCCGACCCACCAGCTCCCGCCCCA CTTGCCCCTCTTGAACCGGATTCTGGTACCAGCTCTGCTGCTGATGGTCCTTGGACACAGAGAGGGGAG GAGGAGGAGGCAGAGGCCAGAGCCAAGCTGGCCCCAGGGAGGGAGCCCCCTAGTCCCTGCCACTCAGAG GACAGCCTTGGGCTGGGGGCAGCACCCCTTGGCAGCGAACCACCCCTGAGCCAGCTGGTGTCCGACTCA GACTCAGAGCTGGACAGCACAGAGCGGCTGGCCCTGGGAAGCACAGACACCTTGTCCAATGGGCAGAAA GCGGACCTGGAGGCTGCGCAGCGCCTGGCCAAGAGGCTGTACCGACTAGATGGCTTCAGGAAGGCCGAT GTGGCCCGGCACCTGGGCAAGAACAATGACTTCAGCAAACTGGTGGCTGGGGAGTACCTCAAGTTCTTT GTCTTCACGGGCATGACTCTGGACCAAGCTCTCAGGGTGTTTCTGAAGGAGCTGGCCTTAATGGGTGAG ACCCAGGAACGAGAGCGCGTGCTGGCCCACTTCTCCCAGCGATACTTCCAGTGCAATCCTGAAGCCCTG TCCTCAGAGGACGGCGCCCACACGCTGACCTGTGCGCTCATGCTGCTCAACACGGATCTCCACGGCCAT AACATCGGGAAGCGCATGACCTGCGGGGACTTCATCGGGAACCTGGAGGGCCTCAATGATGGCGGCGAC TTCCCTAGGGAGCTGCTCAAGGCCTTGTACAGCTCCATCAAGAATGAGAAGCTGCAGTGGGCCATAGAC GAGGAGGAGCTGAGACGCTCTCTGTCTGAGTTGGCCGACCCCAACCCCAAGGTCATCAAGCGGATCAGC GGGGGCAGTGGCAGTGGCTCCAGCCCTTTCCTGGACCTGACTCCCGAGCCTGGGGCTGCCGTCTACAAG CACGGGGCCCTGGTGCGAAAGGTGCACGCAGACCCTGACTGCAGGAAGACACCTCGGGGCAAGCGGGGC TGGAAGAGCTTCCACGGGATCCTCAAGGGCATGATCCTCTACCTGCAGAAGGAGGAGTACAAGCCTGGG AAGGCCCTTTCAGAGACGGAGCTCAAGAATGCCATCAGCATCCACCATGCCCTGGCCACTCGTGCCAGT GACTACAGCAAGAGGCCCCACGTCTTCTACCTGCGCACAGCTGACTGGCGGGTCTTCCTCTTCCAGGCC CCGAGCCTGGAGCAGATGCAGTCCTGGATCACTCGCATCAATGTAGTAGCCGCTATGTTCTCTGCGCCC CCCTTCCCAGCTGCTGTTAGCTCCCAAAAGAAGTTCAGCCGCCCTCTCCTGCCCAGCGCTGCCACCCGC CTCTCCCAGGAGGAGCAGGTGCGGACCCACGAGGCCAAGCTGAAGGCCATGGCAAGTGAGCTGCGGGAG CACCGGGCCGCCCAGCTGGGCAAGAAGGGCCGGGGCAAGGAGGCTGAAGAGCAGCGGCAGAAGGAGGCC TACCTGGAGTTTGAGAAATCCCGCTACAGCACCTATGCAGCGCTGCTTCGGGTCAAGCTGAAGGCAGGC AGTGAGGAGCTGGATGCAGTGGAGGCAGCACTGGCCCAGGCCGGGAGCACAGAGGATGGACTCCCTCCT TCTCACTCCAGTCCCTCCCTGCAGCCCAAACCCTCCAGCCAGCCCCGGGCTCAGCGTCACAGCTCAGAG CCTCGGCCAGGGGCAGGCAGTGGGCGGCGGAAGCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270965
Insert Size	3075 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270965.1
RefSeq Size	3861 bp
RefSeq ORF	3075 bp
Locus ID	5662
UniProt ID	A5PKW4
Protein Pathways	Endocytosis
MW	109.5 kDa
Gene Summary	This gene encodes a Plekstrin homology and SEC7 domains-containing protein that functions as a guanine nucleotide exchange factor. The encoded protein regulates signal transduction by activating ADP-ribosylation factor 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (1).

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