MED4 (NM_001270629) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARC36; DRIP36; HSPC126; TRAP36; VDRIP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332987 representing NM_001270629. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTGGCAATTTCAAGAAACCAAAAGTTGTTACAGGCTGGAGAGGAAAACCAGGTCCTGGAGTTGTTA ATTCACCGAGATGGGGAATTTCAAGAACTAATGAAATTGGCACTTAATCAGGGAAAAATTCATCATGAA ATGCAAGTTTTAGAAAAAGAAGTAGAGAAGAGAGACAGTGATATTCAGCAGCTACAAAAACAGCTAAAG GAAGCAGAACAAATACTGGCAACAGCTGTTTACCAAGCGAAGGAGAAACTCAAGTCAATAGAAAAAGCA AGAAAAGGTGCTATCTCCTCTGAAGAAATAATTAAGTATGCACATAGGATCAGTGCAAGTAATGCTGTA TGTGCTCCACTGACCTGGGTTCCAGGGGACCCCCGGAGACCCTACCCAACTGATTTAGAGATGAGAAGT GGGTTACTGGGTCAGATGAACAATCCTTCCACTAATGGCGTGAATGGCCATTTACCAGGAGATGCACTT GCAGCAGGAAGATTGCCAGATGTCCTTGCTCCACAGTATCCATGGCAGTCAAATGACATGTCGATGAAT ATGTTACCACCAAATCATAGTAGTGACTTTTTGTTGGAACCTCCTGGGCATAATAAAGAAAATGAAGAT GATGTAGAGATTATGTCAACGGACTCCTCAAGCAGTAGTAGTGAGTCTGATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270629
Insert Size	675 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270629.1
RefSeq Size	2181 bp
RefSeq ORF	675 bp
Locus ID	29079
UniProt ID	Q9NPJ6
Protein Families	Druggable Genome, Transcription Factors
MW	24.9 kDa
Gene Summary	This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) uses an alternate 5' exon structure and initiates translation at a downstream in-frame start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.

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