LRRC8E (NM_001268284) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332891 representing NM_001268284. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATCCCAGTGGCCGAGTTCAAGCAGTTCACGGAACAGCAGCCTGCGTTCAAGGTGCTCAAACCCTGG TGGGACGTGCTGGCCGAGTACCTCACCGTGGCCATGCTCATGATTGGGGTCTTTGGCTGCACCCTCCAG GTGACACAGGACAAGATCATCTGTCTACCCAATCATGAGCTCCAGGAGAACTTATCAGAGGCCCCGTGC CAGCAATTGCTGCCTCGGGGGATCCCTGAGCAGATTGGGGCCCTGCAGGAGGTTAAAGGCCTTAAGAAC AATTTGGACCTGCAGCAATACAGCTTTATTAACCAGCTGTGTTATGAGACGGCCCTGCACTGGTATGCC AAGTACTTCCCTTACCTCGTGGTCATTCACACACTCATCTTCATGGTCTGCACCAGTTTCTGGTTCAAG TTCCCTGGCACCAGCTCCAAGATTGAACACTTCATCTCCATCCTGGGCAAGTGTTTCGACTCTCCATGG ACCACCAGGGCCCTATCCGAGGTATCCGGGGAGAACCAGAAGGGCCCAGCAGCCACCGAACGGGCTGCG GCCACCATAGTGGCCATGGCAGGGACCGGGCCGGGGAAGGCAGGGGAGGGTGAGAAGGAGAAAGTGCTG GCGGAACCGGAGAAGGTGGTGACCGAGCCTCCAGTTGTCACCCTGTTGGACAAGAAGGAGGGTGAGCAA GCCAAAGCCCTGTTTGAGAAGGTGAAGAAGTTCCGCATGCACGTGGAAGAGGGCGACATCCTGTACACC ATGTACATCCGACAGACGGTGCTGAAAGTGTGTAAGTTCCTGGCCATCCTGGTCTACAACCTGGTCTAT GTGGAGAAGATCAGTTTCCTGGTGGCCTGTAGGGTGGAGACGTCAGAGGTCACGGGCTACGCCAGCTTC TGCTGCAACCACACCAAGGCCCACCTCTTCTCCAAGCTGGCCTTCTGTTACATCTCCTTTGTGTGCATC TACGGACTTACCTGCATCTACACGCTCTACTGGCTCTTCCACCGGCCCCTCAAGGAGTACTCCTTCCGT TCCGTGCGGGAGGAGACTGGCATGGGGGACATTCCTGACGTCAAGAATGACTTCGCCTTCATGCTGCAC CTCATCGATCAGTACGACTCCCTCTACTCCAAGCGCTTCGCCGTCTTCCTGTCCGAGGTCAGCGAAAGC CGTCTAAAGCAGCTCAATCTCAACCACGAGTGGACGCCCGAGAAGCTTCGACAGAAGCTGCAGCGCAAT GCCGCGGGCCGGCTGGAGCTGGCCCTCTGCATGCTGCCGGGTCTGCCCGACACCGTCTTTGAGCTCAGT GAGGTGGAGTCACTCAGGCTGGAGGCCATCTGCGATATCACCTTCCCCCCGGGGCTGTCACAGCTGGTG CACTTGCAGGAGCTCAGCTTGCTCCACTCGCCCGCCAGGCTACCCTTCTCCTTGCAGGTCTTCCTGCGG GACCACCTGAAGGTGATGCGCGTCAAATGCGAGGAGCTCCGCGAGGTGCCGCTTTGGGTGTTTGGGCTG CGGGGCTTGGAGGAGCTGCACCTGGAGGGGCTTTTCCCCCAGGAGCTAGCTCGGGCAGCCACCCTGGAG AGCCTCCGGGAGCTGAAGCAGCTCAAGGTGTTGTCCCTCCGGAGCAACGCCGGGAAGGTGCCAGCCAGT GTGACCGACGTTGCTGGCCACCTGCAGAGGCTCAGCCTGCACAACGATGGGGCCCGTCTGGTTGCCCTG AACAGCCTCAAGAAGCTGGCGGCATTGCGGGAGCTGGAGCTGGTGGCCTGCGGGCTGGAGCGCATCCCC CATGCAGTGTTCAGCCTGGGTGCGCTGCAGGAACTTGACCTCAAGGACAACCACCTGCGCTCCATCGAG GAAATCCTCAGCTTCCAGCACTGCCGGAAGCTGGTCACGCTCAGGCTGTGGCACAACCAGATCGCCTAC GTCCCTGAGCACGTGCGGAAGCTCAGGAGCCTGGAGCAGCTCTACCTCAGCTACAACAAGCTGGAGACC CTGCCCTCCCAGCTCGGCCTGTGCTCAGGCCTCCGTCTGCTGGATGTGTCCCACAATGGGCTACACTCC CTGCCACCCGAGGTGGGCCTCCTGCAGAACCTACAGCACCTGGCCCTCTCCTACAATGCCCTGGAGGCC CTGCCCGAAGAGCTCTTCTTCTGCCGCAAGCTGCGGACGTTGCTTCTGGGCGACAACCAGCTGAGCCAG CTCTCGCCCCACGTGGGTGCCCTCAGAGCCCTCAGCCGCCTGGAGCTCAAAGGCAACCGCTTAGAGGCG CTGCCAGAAGAACTTGGCAACTGTGGGGGGCTCAAGAAGGCGGGGCTCCTGGTGGAAGACACGCTTTAC CAGGGTCTGCCGGCAGAAGTGCGGGACAAGATGGAGGAGGAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001268284
Insert Size	2391 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001268284.1
RefSeq Size	3766 bp
RefSeq ORF	2391 bp
Locus ID	80131
UniProt ID	Q6NSJ5
Protein Families	Transmembrane
MW	90.2 kDa
Gene Summary	This gene encodes a member of a small, conserved family of proteins with similar structure, including a string of extracellular leucine-rich repeats. A related protein was shown to be involved in B-cell development. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (2) contains an additional exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.