FBH1 (NM_001258452) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Fbx18; FBXO18; hFBH1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332719 representing NM_001258452. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCAAAAGCAATTCTGTTGGCCAGGACAGCTGTCAGGACTCTGAGGGTGACATGATCTTTCCTGCA GAGAGCAGCTGTGCACTGCCTCAGGAAGGCAGTGCAGGGCCGGGCTCACCAGGGTCTGCCCCGCCCTCC AGGAAGCGGTCTTGGTCCTCTGAGGAAGAGAGTAACCAGGCTACCGGGACCAGCCGGTGGGATGGAGTT TCTAAGAAAGCTCCACGGCACCATTTGTCTGTGCCATGCACAAGGCCTAGGGAGGCCAGGCAAGAAGCA GAGGACAGTACGTCTCGGCTCTCTGCGGAGTCTGGTGAAACCGACCAAGATGCTGGGGACGTGGGTCCT GATCCCATTCCTGACTCATACTATGGGCTTCTTGGGACCTTGCCCTGCCAGGAAGCACTGAGCCACATT TGCAGCCTGCCTAGTGAGGTCCTGAGGCACGTGTTTGCCTTCCTCCCGGTGGAAGACCTCTATTGGAAC CTGAGCTTGGTGTGCCACTTGTGGAGGGAGATCATCAGTGACCCGCTGTTCATTCCTTGGAAGAAGCTG TACCATCGATACCTGATGAATGAAGAGCAAGCTGTCAGCAAAGTGGACGGCATCCTGTCTAACTGTGGC ATAGAAAAGGAGTCAGACCTGTGTGTGCTGAACCTCATACGATACACAGCCACCACTAAGTGCTCTCCG AGTGTGGATCCCGAGAGGGTGCTGTGGAGTCTGAGGGACCACCCCCTCCTCCCCGAGGCTGAGGCGTGT GTGCGGCAACACCTCCCCGACCTCTACGCTGCTGCCGGGGGTGTCAACATCTGGGCCCTGGTGGCGGCT GTGGTGCTCCTCTCCAGCAGTGTGAATGACATCCAGCGACTGCTCTTCTGCCTCCGGAGACCCAGCTCC ACGGTGACCATGCCAGATGTCACCGAGACCCTGTACTGCATAGCCGTGCTTCTCTACGCCATGAGGGAG AAGGGGATTAACATCAGCAATAGGATTCACTACAACATTTTCTATTGCCTATATCTTCAGGAGAATTCC TGCACTCAGGCCACAAAAGTTAAAGAGGAGCCATCTGTCTGGCCAGGCAAGAAAACCATCCAACTTACA CATGAACAACAGCTGATTCTGAATCACAAGATGGAACCTCTCCAGGTGGTGAAAATTATGGCCTTTGCC GGCACTGGGAAGACCTCAACGCTGGTCAAGTATGCAGAGAAGTGGTCTCAGAGCAGGTTTCTGTATGTG ACATTCAACAAGAGCATCGCAAAGCAGGCCGAACGCGTCTTCCCCAGCAACGTCATCTGCAAAACCTTC CACTCCATGGCCTACGGGCACATAGGGCGGAAGTACCAGTCAAAGAAGAAGTTGAATCTCTTCAAGTTA ACACCCTTCATGGTCAACTCCGTCCTTGCTGAAGGGAAGGGTGGATTCATAAGAGCCAAGCTTGTGTGT AAGACTCTAGAAAACTTCTTTGCCTCGGCTGACGAAGAGCTGACCATTGATCACGTGCCTATTTGGTGT AAGAACAGCCAAGGACAGAGAGTCATGGTTGAGCAGAGTGAAAAACTGAATGGTGTCCTTGAAGCGAGC CGCCTCTGGGATAACATGCGGAAGCTGGGGGAGTGCACAGAAGAGGCGCACCAGATGACTCATGACGGC TACTTGAAACTCTGGCAGCTGAGCAAGCCTTCGCTGGCCTCTTTTGACGCCATCTTTGTGGATGAGGCC CAGGACTGCACACCAGCTATCATGAACATAGTTCTGTCTCAGCCATGTGGGAAAATCTTTGTAGGGGAC CCGCACCAGCAGATCTATACCTTCCGGGGTGCGGTCAACGCCCTGTTCACAGTGCCCCACACCCACGTC TTCTATCTCACGCAGAGTTTTCGGTTTGGTGTGGAAATAGCTTATGTGGGAGCTACTATCTTGGATGTT TGCAAGAGAGTCAGGAAAAAGACTTTGGTTGGAGGAAACCATCAGAGTGGCATTAGAGGTGACGCAAAG GGGCAAGTGGCCTTGTTGTCCCGGACCAACGCCAACGTGTTTGATGAGGCCGTACGGGTGACGGAAGGG GAATTCCCTTCAAGGATACATTTGATTGGGGGGATTAAATCATTTGGATTGGACAGAATCATTGATATT TGGATCCTTCTTCAGCCAGAGGAAGAACGGAGGAAACAAAACCTCGTCATTAAAGACAAATTTATCAGA AGATGGGTGCACAAAGAAGGCTTTAGTGGCTTCAAGAGGTATGTGACCGCTGCCGAGGACAAGGAGCTT GAAGCCAAGATCGCAGTTGTTGAAAAGTATAACATCAGGATTCCAGAGCTGGTGCAAAGGATAGAAAAA TGCCATATAGAAGATTTGGACTTTGCAGAGATGGAGTCTCACCATGTTGGCCAGGCTGGTCTCGAACTC CCAACCTCAGAGTACATTCTGGGCACTGTGCACAAAGCCAAAGGCCTGGAGTTTGACACTGTGCATGTT TTGGATGATTTTGTGAAAGTGCCTTGTGCCCGGCATAACCTGCCCCAGCTTCCGCACTTCAGAGTTGAG TCATTTTCTGAGGATGAATGGAATTTACTGTATGTTGCAGTAACTCGAGCCAAGAAGCGTCTCATCATG ACCAAATCATTGGAAAACATTTTGACTTTGGCTGGGGAGTACTTCTTGCAAGCAGAGCTGACAAGCAAC GTCTTAAAAACAGGCGTGGTGCGCTGCTGCGTGGGACAGTGCAACAATGCCATCCCTGTTGACACCGTC CTTACCATGAAGAAGCTGCCCATCACCTATAGCAACAGGAAGGAAAACAAGGGGGGCTACCTCTGCCAC TCCTGTGCGGAGCAGCGCATCGGGCCCCTGGCGTTCCTGACAGCCTCCCCGGAGCAGGTGCGCGCCATG GAGCGCACTGTGGAGAACATCGTACTGCCCCGGCATGAGGCCCTGCTCTTCCTCGTCTTCTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001258452
Insert Size	2961 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258452.1
RefSeq Size	3681 bp
RefSeq ORF	2961 bp
Locus ID	84893
UniProt ID	Q8NFZ0
Protein Families	Druggable Genome
MW	110.9 kDa
Gene Summary	This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbx class. It contains an F-box motif and seven conserved helicase motifs, and has both DNA-dependent ATPase and DNA unwinding activities. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) differs at the 5' end and contains an additional in-frame coding exon compared to variant 1, which results in translation initiation from an in-frame downstream AUG, and a shorter isoform (3) with an internal protein segment not found in isoform 1.

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