ARHGAP20 (NM_001258415) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RARHOGAP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332695 representing NM_001258415. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTGCCCGGGAGCGGCAGCCGGCGCTGAAGAAGAAAATGAAAACACTAGCAGAAAGGAGGAGGAGC GCTCCATCTCTTATCCTGGATAAAGCCCTACAAAAACGGCCTACTACCAGGGACAGTCCTTCTGCTAGT GTTGACACATGCACATTTCTGTCATCATTAGTGTGCTCCAATAGGACTCTGCTGATTGATGGCCGGGCA GAACTCAAAAGAGGCCTCCAGAGGCAGGAGCGGCATCTTTTCCTATTCAATGATCTGTTTGTTGTGGCC AAAATCAAATATAACAATAACTTTAAGATAAAAAATAAAATTAAATTAACTGATATGTGGACAGCAAGC TGTGTGGATGAAGTGGGAGAAGGCAACACCAATGCCATGAAATCCTTTGTTTTGGGCTGGCCCACAGTG AACTTTGTGGCCACTTTCAGTTCTCCAGAACAAAAGGACAAATGGCTCTCTCTCCTTCAGAGATACATC AATCTAGAGAAAGAAAAGGACTACCCGAAGAGCATTCCCCTCAAAATCTTCGCCAAGGACATTGGGAAT TGTGCCTACTCTAAAACTATAACAGTAATGAATTCAGATACAGCGAATGAAGTTATCAACATGTCATTA CCAATGCTAGGGATAACTGGCTCTGAGAGAGATTACCAGTTGTGGGTCAATTCTGGCAAAGAAGAGGCT CCATACCCACTCATTGGGCATGAATATCCATATGGAATTAAAATGAGCCATCTTCGAGACTCTGCACTC CTGACACCGGGATCAAAGGACTCTACCACCCCTTTCAACCTCCAGGAGCCCTTCCTTATGGAACAGCTC CCCCGAGAGATGCAGTGCCAGTTCATCCTGAAGCCCAGCCGCCTGGCTGCAGCCCAGCAACTGAGTGAT TCAGGTCATAAGACATTTAAAAGGAGAAGATCTATCATAAACTGGGCCTTCTGGCGAGGTTCTAGCACT CACCTGGACAACTTGCCCTCATCGCCAACATCACCTATGCCAGGACAGCTCTTTGGAATTTCTCTGCCA AATATTTGTGAGAATGACAATCTGCCCAAACCTGTCTTGGATATGCTTTTCTTTCTTAATCAAAAAGGA CCTCTCACCAAAGGTATCTTCAGGCAATCAGCCAATGTGAAATCCTGCAGAGAACTAAAAGAGAAATTG AATTCTGGAGTCGAAGTACACCTAGACTGTGAATCTATTTTTGTGATAGCATCTGTCTTAAAGGATTTT CTGCGAAATATTCCAGGAAGTATTTTTTCATCAGATCTCTATGATCACTGGGTCTCTGTAATGGATCAA GGAAATGATGAAGAGAAAATAAATACTGTTCAAAGGCTATTAGACCAGCTTCCGAGAGCCAATGTTGTT CTCCTAAGGTATCTTTTTGGGGTGTTACACAACATTGAGCAACATTCCTCATCCAATCAGATGACTGCA TTTAATTTAGCTGTGTGTGTCGCTCCAAGTATTCTTTGGCCTCCTGCTTCCTCCAGCCCAGAACTAGAA AACGAATTTACAAAAAAGGTTTCCCTGCTTATACAATTTCTGATTGAGAATTGCCTTAGGATATTTGGA GAAGAAATCACTTCCCTCTTCAGAGAGGTTTCAGTGAGATGTGACACTAGAGAGAATGCCTCAGATATT TCTTGCTTTCAACTGAATGACTCCTCCTATGACAGCTTGGAAAATGAGCTAAATGAGGATGTTGATGCA CCATGCAGTGACTTGGTAAAGAAACTTGGCCAGGGGAGCAGAAGCATGGACTCTGTCTTAACCCTCAGT GACTATGATCTTGACCAGCCCGAGGTGGAAGGCCTTTTAACCCTAAGCGACTTTGACTTGGCCCATTCT AAAGATGAAGATGTTCAAATGAAACGGCCTCTTGAATCCAAGCCGGTGAACATTTTAGTGTACACAAAG ATCCCACTGCGGGATCATGCCAGGGCCCCATCTGCCATGTGCACACCCAGCTACCTGTCCACAGCTGCA GCAAATGCTGCAAAAAGCCTGAGGCGACACCGGCGTTGCTCAGAGCCCAGCATCGACTATCTGGATTCA AAGCTTTCCTACCTCAGGGAGTTTTATCAGAAAAAGCTACGCAAGTCCAGCTGTGATGCAATTCTTTCT CAAAAAGATGAAGACTATCTGAAGCAGAATCAACCCCTCCAGGAGGAAGGAAAGACATGTTTTAAACAG AGTTTAGTCACAGGCACTGATGTCAGCAAGAAAAATGCCACTACTCAAAACACTAAGAAGAAAAGCTTG TCTGGTAGTGAAGGAAATCACGTGAAACTTTTCCCTAAGTCTAAGCCAGTGGCCATTTCTGTGGCATCT TATAGTCCTATGTCCTCACAGGATCATTCCAAGAACCAGCCCTTTGATGTGAATACATCTGGATACTCC CCACCACACACAGCAGATGCCCTCAAGGGTCCAAGGACACATCGGCGCTGCTCAGAGCCCAACATAGAA GACCAGAACCGCAAGCTGACCTATCTCAGGGGAATTTATTCAAAGAAACAACATAAAACCAGCTGTGAA GCTGGTCTCTTGCATGGAGAGGAGGATTATCTCAAACGGCATAAGTCTTTGCAAATGGAGGGGCAGAAG CTCATTAATCAGAGTTTAGTCATGGGGATTGAGGTGGGCAAGAGTAGTGCCACAAACCAAAACACTGAG AAGGTTTTACCCCCAAGATTAAACCTTTGCCCAAGGACCAGCTATTCCAGCTTATCCTCCCCAGGCACT TCCCCATCCGGCTCATCAGTAAGCTCCCAAGACAGTGCTTTTTCTCAGATTTCTGAACACTCTGTGTTT ACACCCACTGAGACTTCCTCTCCAATAGATTGCACTTTTCAGGCTCAGAGAAAACGGGAAGACCTTTCT CCTGACTTTAGCAATGCCAGCCATGTTTCCGGAATGCCCGGTCCCTCATCAGGGCAGGCTTGCAGCCGC CCAGCCTATACAAAGAAGGACACCATGGAGTGGCATTCACAAATGCATTCTGTAACTCTTCATCCCAGC ACATGGTTGAGAAATGGTGTGGCCAGTTTGAAAAACTGGTCCCTCAAAAAGAAAGCAAAGGCAGCCAGA CCAGAGGAAGAGAAAATAGCTTCTCCAAAAGGACCCTTAGAGCCACCCCCACATGCTTCTGGTGTTCCA GAAGCCAACTCACTGCAAGAGGAACAAAAAGACTTGCCCTTAAGGGCAGCTGAAGGACTGTCCCCTGTG CAGTCAGCCCAAAGGTGTAGTTCTTCTCCCTTCCAGGACTCAGAGAGACACTGTAGCTCTCCATTCAGC CTGGTGGAGAGCAGACTTAAGCTGTGCATGAAGTCACATGAGGAAATAGAGCCTGGTAGTCAGAGCTCT TCTGGTTCTCTGCCTTGGGAAAGAGCCTCAGCCAGCTCTTGGACTCTAGAGGATGCGACCAGCCCAGAC TCAGGGCCTACAGTGGTCTGCGACATTGAGGACAGGTATTTAACCAAAGACATTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258415
Insert Size	3507 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258415.1
RefSeq Size	6199 bp
RefSeq ORF	3507 bp
Locus ID	57569
UniProt ID	Q9P2F6
MW	130.5 kDa
Gene Summary	The protein encoded by this gene is an activator of RHO-type GTPases, transducing a signal from RAP1 to RHO and impacting neurite outgrowth. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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