NFAT1 (NFATC2) (NM_001258296) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NFAT1; NFATP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332642 representing NM_001258296. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCACCTCGAACCAGCCTCGCCGAGGACAGCTGCCTGGGCCGCCACTCGCCCGTGCCCCGTCCGGCC TCCCGCTCCTCATCGCCTGGTGCCAAGCGGAGGCATTCGTGCGCCGAGGCCTTGGTTGCCCTGCCGCCC GGAGCCTCACCCCAGCGCTCCCGGAGCCCCTCGCCGCAGCCCTCATCTCACGTGGCACCCCAGGACCAC GGCTCCCCGGCTGGGTACCCCCCTGTGGCTGGCTCTGCCGTGATCATGGATGCCCTGAACAGCCTCGCC ACGGACTCGCCTTGTGGGATCCCCCCCAAGATGTGGAAGACCAGCCCTGACCCCTCGCCGGTGTCTGCC GCCCCATCCAAGGCCGGCCTGCCTCGCCACATCTACCCGGCCGTGGAGTTCCTGGGGCCCTGCGAGCAG GGCGAGAGGAGAAACTCGGCTCCAGAATCCATCCTGCTGGTTCCGCCCACTTGGCCCAAGCCGCTGGTG CCTGCCATTCCCATCTGCAGCATCCCAGTGACTGCATCCCTCCCTCCACTTGAGTGGCCGCTGTCCAGT CAGTCAGGCTCTTACGAGCTGCGGATCGAGGTGCAGCCCAAGCCACATCACCGGGCCCACTATGAGACA GAAGGCAGCCGAGGGGCTGTCAAAGCTCCAACTGGAGGCCACCCTGTGGTTCAGCTCCATGGCTACATG GAAAACAAGCCTCTGGGACTTCAGATCTTCATTGGGACAGCTGATGAGCGGATCCTTAAGCCGCACGCC TTCTACCAGGTGCACCGAATCACGGGGAAAACTGTCACCACCACCAGCTATGAGAAGATAGTGGGCAAC ACCAAAGTCCTGGAGATACCCTTGGAGCCCAAAAACAACATGAGGGCAACCATCGACTGTGCGGGGATC TTGAAGCTTAGAAACGCCGACATTGAGCTGCGGAAAGGCGAGACGGACATTGGAAGAAAGAACACGCGG GTGAGACTGGTTTTCCGAGTTCACATCCCAGAGTCCAGTGGCAGAATCGTCTCTTTACAGACTGCATCT AACCCCATCGAGTGCTCCCAGCGATCTGCTCACGAGCTGCCCATGGTTGAAAGACAAGACACAGACAGC TGCCTGGTCTATGGCGGCCAGCAAATGATCCTCACGGGGCAGAACTTTACATCCGAGTCCAAAGTTGTG TTTACTGAGAAGACCACAGATGGACAGCAAATTTGGGAGATGGAAGCCACGGTGGATAAGGACAAGAGC CAGCCCAACATGCTTTTTGTTGAGATCCCTGAATATCGGAACAAGCATATCCGCACACCTGTAAAAGTG AACTTCTACGTCATCAATGGGAAGAGAAAACGAAGTCAGCCTCAGCACTTTACCTACCACCCAGTCCCA GCCATCAAGACGGAGCCCACGGATGAATATGACCCCACTCTGATCTGCAGCCCCACCCATGGAGGCCTG GGGAGCCAGCCTTACTACCCCCAGCACCCGATGGTGGCCGAGTCCCCCTCCTGCCTCGTGGCCACCATG GCTCCCTGCCAGCAGTTCCGCACGGGGCTCTCATCCCCTGACGCCCGCTACCAGCAACAGAACCCAGCG GCCGTACTCTACCAGCGGAGCAAGAGCCTGAGCCCCAGCCTGCTGGGCTATCAGCAGCCGGCCCTCATG GCCGCCCCGCTGTCCCTTGCGGACGCTCACCGCTCTGTGCTGGTGCACGCCGGCTCCCAGGGCCAGAGC TCAGCCCTGCTCCACCCCTCTCCGACCAACCAGCAGGCCTCGCCTGTGATCCACTACTCACCCACCAAC CAGCAGCTGCGCTGCGGAAGCCACCAGGAGTTCCAGCACATCATGTACTGCGAGAATTTCGCACCAGGC ACCACCAGACCTGGCCCGCCCCCGGTCAGTCAAGGTCAGAGGCTGAGCCCGGGTTCCTACCCCACAGTC ATTCAGCAGCAGAATGCCACGAGCCAAAGAGCCGCCAAAAACGGACCCCCGGTCAGTGACCAAAAGGAA GTATTACCTGCGGGGGTGACCATTAAACAGGAGCAGAACTTGGACCAGACCTACTTGGATGATGAGCTG ATAGACACACACCTTAGCTGGATACAAAACATATTATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001258296
Insert Size	2109 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258296.1
RefSeq Size	7016 bp
RefSeq ORF	2109 bp
Locus ID	4773
UniProt ID	Q13469
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
MW	76.7 kDa
Gene Summary	This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (7) uses an alternate splice junction at the 5' end of a coding exon and contains an alternate exon in the 3' end compared to variant 2, that causes a frameshift. The resulting isoform (F) is shorter at the N-terminus and has a shorter and distinct C-terminus compared to isoform C. Variants 5 and 7 both encode the same isoform (F). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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