HTF9C (TRMT2A) (NM_001257994) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HTF9C
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332558 representing NM_001257994. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGTGAGAACCTCGACAACGAGGGCCCGAAGCCCATGGAGAGCTGTGGCCAGGAGAGCAGCAGTGCC CTGAGCTGCCCTACCGTCTCGGTGCCCCCTGCAGCCCCGGCAGCCCTGGAGGAGGTGGAGAAAGAGGGC GCTGGGGCGGCTACAGGGCCGGGGCCTCAGCCCGGGCTCTACAGCTACATCAGGGATGACTTGTTTACC TCTGAGATCTTTAAACTGGAGCTGCAGAACGTGCCTCGCCACGCCAGCTTCAGCGACGTCCGGCGCTTC CTGGGCCGCTTTGGTCTGCAGCCCCACAAAACCAAACTCTTTGGGCAACCACCCTGCGCCTTTGTGACA TTCCGCAGCGCTGCAGAGAGGGACAAGGCCCTGCGCGTTTTGCATGGTGCCCTCTGGAAAGGCCGCCCA CTCAGTGTGCGCCTGGCCCGGCCCAAGGCCGACCCCATGGCCAGGAGGAGGCGACAGGAGGGTGAGAGT GAGCCACCAGTAACACGAGTGGCCGACGTGGTGACCCCTCTATGGACAGTGCCCTATGCTGAGCAGCTT GAGCGGAAGCAGCTGGAGTGCGAGCAGGTGCTGCAGAAACTTGCCAAGGAAATCGGGAGCACCAACCGT GCCTTGCTGCCCTGGCTGCTCGAGCAGAGGCACAAGCACAACAAGGCCTGCTGCCCGCTGGAGGGGGTC AGGCCATCACCCCAGCAGACTGAGTATCGTAATAAGTGTGAGTTTCTGGTTGGCGTCGGGGTGGATGGG GAGGATAACACCGTGGGCTGTCGGCTCGGCAAGTACAAGGGCGGGACGTGTGCTGTGGCAGCCCCGTTT GACACCGTGCACATCCCCGAAGCCACCAAGCAGGTGGTGAAGGCCTTCCAGGAGTTCATCCGGTCCACT CCATACTCGGCATACGACCCAGAGACGTACACAGGCCACTGGAAGCAGCTGACTGTGCGCACCAGCCGC CGCCACCAGGCCATGGCCATTGCCTACTTCCACCCCCAGAAGCTGAGCCCTGAGGAGCTGGCAGAGCTG AAGACCTCCCTAGCGCAGCACTTCACAGCAGGGCCAGGCAGGGCCAGTGGAGTGACCTGCCTCTACTTC GTGGAGGAGGGACAGCGAAAGACTCCTAGCCAGGAGGGCCTGCCCCTGGAGCATGTGGCTGGGGACCGG TGCATCCACGAGGACCTGCTAGGGCTGACCTTCCGGATCTCTCCACACGCCTTCTTCCAGGTGAACACA CCCGCAGCCGAGGTGCTCTACACAGTCATCCAGGACTGGGCCCAATTGGATGCGGGGAGCATGGTGCTG GACGTGTGCTGTGGCACCGGCACCATTGGCCTGGCCCTGGCCCGGAAGGTAAAGAGGGTCATTGGGGTC GAGCTATGCCCAGAGGCTGTGGAGGACGCCCGGGTGAACGCCCAGGACAATGAGTTGAGTAATGTGGAG TTCCACTGCGGGAGGGCCGAGGACCTGGTGCCCACCCTGGTGAGCAGACTGGCCTCCCAGCACCTCGTG GCCATCCTGGACCCACCCCGTGCTGGCTTGCATTCCAAGGTGATCCTGGCCATCCGGAGAGCTAAGAAC CTCAGGCGGCTGCTGTACGTCTCATGCAACCCCCGGGCAGCCATGGGCAACTTTGTGGACGCCCCCCTT TTCCCCCCACAGCCTCTGCAGAGCCCCATCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257994
Insert Size	1689 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257994.1
RefSeq Size	3003 bp
RefSeq ORF	1689 bp
Locus ID	27037
UniProt ID	Q8IZ69
Protein Families	Druggable Genome
MW	62 kDa
Gene Summary	The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (3) uses an alternate splice junction at the 5' end of a coding exon compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus compared to isoform a.

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