MYPN (NM_001256267) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CMD1DD; CMH22; MYOP; NEM11; RCM4
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332329 representing NM_001256267. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCAAGACGACAGCATAGAAGCTTCTACTTCCATATCTCAGCTTCTAAGAGAGAGCTATTTAGCTGAA ACCAGACATCGGGGAAACAATGAGAGGAGTCGAGCGGAGCCCTCCTCCAACCCTTGCCATTTCGGCAGT CCTTCTGGGGCCGCTGAAGGAGGCGGAGGCCAAGATGACCTTCCAGATCTTTCAGCCTTTCTGAGCCAA GAAGAATTAGACGAAAGTGTCAATTTGGCAAGACTGGCCATCAATTACGACCCTTTGGAGAAGGCAGAT GAAACTCAAGCTAGAAAACGACTTTCTCCTGATCAGATGAAACACTCACCTAATTTAAGTTTTGAGCCT AACTTCTGCCAGGATAACCCTCGAAGTCCCACCAGCTCTAAAGAAAGCCCCCAGGAGGCAAAAAGGCCA CAGTATTGTTCTGAAACCCAGTCCAAAAAAGTATTTTTAAATAAGGCTGCCGACTTCATTGAAGAGCTA TCCTCCCTTTTCAAATCCCACAGCTCCAAAAGGATTAGACCTCGTGCCTGCAAAAACCACAAGAGTAAA CTGGAATCTCAAAACAAAGTTATGCAGGAAAACAGCTCCAGTTTCTCAGATCTGTCAGAAAGACGAGAA AGATCTTCTGTTCCCATCCCTATCCCTGCGGATACCAGGGATAATGAAGTGAATCACGCCCTGGAACAG CAGGAAGCCAAGAGGCGTGAAGCGGAGCAGGCTGCCAGTGAGGCGGCTGGTGGAGACACTACACCAGGG TCTTCCCCTTCATCTCTGTACTATGAAGAACCTCTGGGGCAACCTCCCCGGTTCACTCAAAAGTTACGG AGCAGAGAAGTTCCAGAAGGAACTCGAGTACAGTTGGATTGCATAGTGGTAGGAATTCCACCACCTCAA GTAAGGTGGTACTGTGAAGGCAAGGAGCTTGAAAATTCCCCAGATATTCACATCGTCCAGGCAGGAAAT CTGCACTCACTGACCATTGCGGAAGCCTTTGAAGAGGACACAGGACGCTATTCCTGCTTTGCTTCTAAC ATCTATGGGACAGATTCGACTTCTGCTGAGATTTATATAGAAGGGGTTTCTTCTTCTGACTCAGAAGGC GACCCTAACAAGGAAGAGATGAATCGAATCCAGAAGCCAAATGAGGTGTCATCTCCTCCCACTACCTCT GCAGTCATTCCTCCAGCAGTACCCCAAGCCCAGCATTTGGTGGCCCAACCTCGTGTGGCAACCATCCAG CAGTGTCAGAGCCCCACCAATTACTTGCAGGGATTGGATGGAAAACCTATCATTGCAGCTCCTGTGTTT ACAAAGATGCTACAAAATTTGTCAGCTTCTGAGGGTCAGCTGGTTGTCTTTGAATGCAGAGTAAAAGGA GCTCCATCTCCTAAGGTTGAGTGGTATAGAGAAGGGACTTTAATAGAAGATTCTCCAGATTTTAGGATT TTACAGAAAAAACCTCGATCCATGGCAGAGCCAGAGGAGATTTGCACCTTGGTCATTGCTGAGGTGTTT GCAGAAGATTCTGGGTGCTTCACATGTACTGCAAGCAACAAATACGGCACAGTGTCAAGCATTGCACAG CTGCACGTGAGAGGAAATGAGGACCTCAGCAACAACGGGTCTCTTCACTCAGCCAACTCTACCACCAAC CTGGCAGCTATTGAGCCACAGCCCTCCCCACCCCACTCAGAGCCTCCATCTGTGGAACAACCCCCCAAA CCCAAACTCGAGGGGGTTCTGGTGAACCACAATGAGCCCCGGTCCAGCTCCAGGATTGGGCTTCGTGTG CACTTCAACCTGCCTGAAGATGACAAAGGAAGTGAAGCATCCTCCGAGGCTGGTGTGGTGACCACCAGA CAGACCAGGCCCGATTCTTTCCAGGAGAGGTTCAACGGACAGGCAACAAAAACCCCAGAGCCTTCTTCC CCCGTGAAAGAGCCCCCTCCAGTTCTGGCCAAACCCAAACTTGATTCCACTCAGTTACAACAGCTTCAT AACCAAGTCTTACTGGAACAACACCAATTGCAAAACCCACCTCCTTCATCTCCTAAGGAGTTTCCTTTC AGCATGACTGTTTTGAACTCCAATGCTCCCCCAGCGGTGACAACATCCAGTAAGCAGGTGAAGGCTCCT TCATCACAGACGTTCAGCTTGGCCCGGCCGAAGTATTTCTTCCCCTCCACGAACACCACCGCAGCAACT GTGGCCCCTTCCAGCTCTCCGGTGTTCACTTTGAGCAGCACTCCTCAAACTATTCAGAGGACAGTGAGC AAAGAAAGCCTCTTAGTGTCTCACCCCTCTGTGCAAACCAAATCTCCAGGAGGGCTTTCCATCCAAAAT GAGCCACTCCCACCAGGCCCAACAGAACCAACACCACCACCATTCACATTTTCCATCCCCAGCGGAAAC CAGTTTCAGCCCCGCTGTGTGTCCCCAATTCCTGTCTCTCCTACCAGCCGGATTCAGAACCCAGTGGCT TTCCTCAGCTCTGTTCTGCCTTCTCTCCCTGCCATCCCACCCACAAATGCCATGGGGCTGCCTAGAAGT GCACCATCCATGCCATCCCAGGGATTAGCGAAGAAAAATACAAAGTCTCCTCAACCAGTGAATGATGAT AACATTCGTGAAACTAAGAACGCAGTGATTCGAGACTTGGGGAAAAAAATAACTTTCAGTGATGTCAGA CCAAACCAGCAGGAGTACAAAATTTCAAGCTTTGAGCAGAGGCTGATGAATGAAATAGAGTTTCGCTTG GAACGTACTCCTGTTGATGAATCAGATGATGAAATTCAACATGATGAGATCCCCACGGGCAAGTGTATT GCTCCCATCTTTGACAAGAGACTCAAGCACTTCCGGGTCACAGAAGGCTCTCCAGTCACATTCACCTGC AAAATTGTTGGGATACCTGTTCCAAAGGTTTACTGGTTCAAAGATGGGAAGCAGATTTCTAAGAGAAAT GAGCACTGCAAAATGAGGCGAGAAGGAGATGGGACATGCTCTCTGCACATTGAATCCACTACCAGTGAT GACGATGGCAACTACACCATCATGGCAGCCAACCCCCAGGGGAGAATCAGCTGTTCTGGCCACTTGATG GTACAAAGTTTGCCCATTCGCAGTCGGCTAACCTCTGCTGGTCAGTCTCACAGGGGAAGATCCCGAGTG CAAGAAAGAGACAAAGAGCCCCTACAGGAACGCTTTTTCCGACCACATTTCCTGCAGGCTCCTGGGGAT ATGGTAGCTCATGAGGGGCGCCTCTGTCGGCTGGACTGTAAGGTGAGTGGTTTACCGCCCCCGGAGCTG ACATGGCTACTCAATGGCCAACCTGTGCTACCAGATGCCTCCCACAAGATGCTGGTCAGGGAGACCGGA GTCCACTCTCTGCTCATTGACCCACTCACTCAGCGCGACGCAGGGACCTATAAGTGCATCGCTACCAAC AAAACCGGGCAGAATTCTTTTAGTCTGGAGCTCTCTGTAGTAGCCAAAGAGGTGAAGAAAGCACCTGTG ATCCTGGAGAAACTACAGAACTGCGGTGTTCCCGAAGGCCACCCCGTGAGACTGGAGTGCCGCGTGATA GGCATGCCCCCACCTGTGTTCTACTGGAAGAAAGACAATGAGACCATCCCTTGCACCAGAGAGAGGATC AGTATGCACCAGGACACAACAGGGTATGCCTGCCTTCTCATTCAGCCAGCCAAGAAATCAGACGCTGGA TGGTACACGTTGTCAGCCAAGAATGAAGCCGGCATCGTGTCGTGCACTGCCAGGCTGGATATATACGCT CAGTGGCACCATCAGATCCCACCGCCCATGTCTGTCCGGCCCAGTGGCAGTCGCTACGGATCTCTCACC AGTAAAGGACTTGACATATTTTCTGCCTTTTCCTCCATGGAAAGCACGATGGTGTATTCATGCTCTTCT CGGAGTGTAGTGGAGAGTGATGAACTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256267
Insert Size	3963 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256267.1
RefSeq Size	5725 bp
RefSeq ORF	3963 bp
Locus ID	84665
UniProt ID	Q86TC9
MW	145.3 kDa
Gene Summary	Striated muscle in vertebrates comprises large proteins which must be organized properly to contract efficiently. Z-lines in striated muscle are a sign of this organization, representing the ends of actin thin filaments, titin, nebulin or nebulette and accessory proteins required for structure and function. This gene encodes a protein which interacts with nebulin in skeletal muscle or nebulette in cardiac muscle and alpha-actinin. In addition, this gene product can interact with a protein with the I-band indicating it has a regulatory as well as structural function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Both variants 1 and 4 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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