CD101 (NM_001256106) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EWI-101; IGSF2; V7
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332296 representing NM_001256106. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAGGCATCTCATATGTGGCATCTTTCTTTCTCCTTCTGACTAAGCTCAGCATTGGCCAGAGAGAA GTAACAGTTCAGAAAGGACCACTGTTTAGAGCTGAAGGTTACCCAGTCAGCATTGGCTGCAATGTAACT GGCCACCAGGGACCTTCTGAGCAGCATTTCCAGTGGTCTGTTTACCTGCCGACAAACCCGACCCAGGAA GTCCAGATCATTAGCACCAAGGATGCTGCCTTCTCTTACGCAGTATATACGCAGCGGGTGCGAAGCGGA GACGTCTACGTGGAGAGGGTCCAGGGCAACTCAGTCTTGTTGCACATCTCAAAACTCCAGATGAAGGAT GCTGGCGAGTATGAGTGTCACACACCAAACACTGATGAGAAATACTATGGAAGTTACAGTGCAAAGACT AATCTAATTGTTATTCCAGATACCCTCTCTGCCACCATGAGTTCTCAGACTCTCGGTAAGGAGGAAGGT GAGCCATTAGCCCTCACCTGTGAGGCATCCAAAGCCACAGCCCAACATACTCACCTCTCTGTCACCTGG TACCTAACACAGGATGGAGGAGGAAGCCAAGCCACTGAGATTATTTCTCTCTCCAAAGATTTTATATTG GTCCCTGGGCCCTTGTATACAGAGCGGTTTGCAGCCAGTGACGTACAGCTCAACAAACTGGGACCCACT ACATTCAGGCTGTCCATAGAGAGGCTCCAGTCCTCAGATCAGGGTCAGCTGTTCTGTGAGGCAACGGAA TGGATTCAGGATCCAGATGAAACTTGGATGTTCATCACCAAAAAGCAGACCGATCAAACCACTCTGAGG ATCCAGCCAGCAGTGAAAGATTTTCAAGTCAACATTACAGCTGACAGCTTGTTTGCTGAAGGGAAACCC TTAGAACTGGTTTGCCTGGTTGTAAGCAGTGGCCGTGACCCACAGCTTCAAGGCATTTGGTTCTTCAAT GGGACTGAAATTGCTCACATTGATGCTGGTGGAGTCCTGGGCCTGAAGAATGACTACAAAGAGAGAGCA AGTCAAGGAGAGCTCCAGGTTTCAAAGTTAGGCCCCAAGGCTTTCTCTCTCAAGATCTTCTCTCTGGGC CCAGAGGATGAAGGCGCCTACAGATGTGTGGTAGCAGAGGTCATGAAAACACGCACAGGTTCCTGGCAG GTGCTTCAGAGAAAGCAGTCACCAGACAGCCACGTGCACCTGAGGAAGCCAGCAGCAAGAAGTGTGGTC ATGTCTACCAAGAACAAGCAGCAAGTTGTGTGGGAAGGAGAGACACTCGCCTTTCTCTGTAAGGCTGGT GGAGCTGAAAGTCCCCTGTCTGTGAGCTGGTGGCACATCCCACGGGACCAGACACAGCCCGAGTTTGTG GCTGGCATGGGGCAGGATGGCATTGTGCAGCTGGGTGCCTCCTATGGGGTACCCAGTTACCATGGCAAC ACAAGGCTGGAGAAAATGGACTGGGCCACCTTCCAGCTGGAGATCACCTTCACTGCCATCACAGACAGT GGCACATATGAGTGCAGAGTATCTGAGAAGTCTCGGAACCAGGCCAGAGATCTGAGCTGGACTCAGAAG ATTTCAGTTACTGTAAAGTCTCTGGAGTCAAGTTTACAAGTTAGTCTGATGAGCCGTCAGCCGCAAGTG ATGTTAACCAACACCTTTGACCTGTCCTGTGTCGTGAGGGCCGGTTACTCTGACCTCAAGGTGCCACTC ACTGTGACGTGGCAGTTCCAGCCAGCTAGCTCTCACATCTTCCACCAGCTTATTCGAATCACCCACAAT GGCACTATTGAATGGGGGAATTTCCTATCCCGGTTCCAAAAGAAGACGAAAGTGTCGCAGTCTTTATTT CGTTCACAACTCCTAGTCCATGATGCCACTGAGGAAGAGACAGGAGTGTATCAGTGTGAAGTAGAAGTT TATGACAGAAATTCCCTATACAACAACCGCCCCCCGAGGGCTTCTGCCATCTCTCACCCACTGAGGATA GCCGTCACTTTACCAGAGAGCAAGCTAAAAGTGAATTCAAGGAGTCAAGTCCAAGAGCTCTCCATCAAC TCCAACACTGATATAGAATGTAGCATCTTGTCCCGGTCCAATGGAAACCTTCAGTTAGCCATTATTTGG TATTTTTCTCCTGTTTCCACTAATGCCTCTTGGCTAAAGATCCTGGAGATGGACCAAACCAATGTTATA AAAACTGGGGATGAGTTTCACACCCCACAGAGAAAACAAAAATTTCATACTGAGAAGGTTTCCCAAGAC TTATTTCAGCTGCACATTCTGAATGTGGAAGACAGCGATCGGGGCAAATATCACTGTGCTGTGGAGGAA TGGCTCCTGTCTACAAATGGCACTTGGCACAAGCTTGGAGAAAAGAAGTCAGGACTAACAGAATTGAAA CTCAAGCCCACAGGAAGTAAGGTACGTGTCTCCAAAGTGTACTGGACCGAAAATGTGACTGAGCACAGA GAAGTGGCCATCCGCTGCAGCCTGGAGAGTGTAGGCAGCTCAGCCACTCTGTACTCTGTGATGTGGTAC TGGAACAGAGAAAACTCTGGAAGTAAATTGCTGGTGCACTTGCAACATGATGGCTTGCTGGAGTATGGG GAAGAGGGGCTCAGGAGGCACCTGCACTGTTACCGTTCATCCTCTACAGACTTTGTCCTGAAGCTTCAT CAGGTGGAGATGGAGGATGCAGGAATGTACTGGTGTAGGGTGGCAGAGTGGCAGCTCCATGGACACCCA AGCAAGTGGATTAATCAAGCATCCGATGAGTCACAGCGGATGGTGCTCACGGTGCTGCCTTCAGAGCCC ACGCTTCCTTCCAGGATCTGCTCCTCGGCCCCTTTACTCTATTTCCTGTTCATCTGTCCCTTCGTCCTG CTCCTCCTTCTGCTCATCTCCCTCCTCTGCTTATACTGGAAGGCCAGGAAGTTGTCAACACTGCGTTCC AACACACGGAAAGAAAAAGCTCTCTGGGTGGACTTGAAAGAGGCTGGAGGTGTGACCACAAATAGGAGG GAAGACGAGGAGGAAGATGAAGGCAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256106
Insert Size	3066 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256106.2
RefSeq Size	3551 bp
RefSeq ORF	3066 bp
Locus ID	9398
UniProt ID	Q93033
Protein Families	Transmembrane
MW	115.1 kDa
Gene Summary	Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 3' UTR, compared to variant 1. Variants 1, 2 and 3 all encode isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.