HINFP (NM_001243259) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HiNF-P; MIZF; ZNF743
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331984 representing NM_001243259. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCGCCTCCTGGGAAAGTTCCCCGAAAGGAGAATCTGTGGCTACAGTGTGAGTGGGGGTCCTGCTCC TTTGTGTGCTCAACCATGGAAAAGTTCTTTGAGCATGTCACTCAGCACCTGCAGCAGCACCTGCATGGC TCTGGGGAGGAGGAGGAAGAGGAAGAGGAGGATGACCCACTTGAGGAAGAATTCTCCTGCTTGTGGCAG GAATGTGGCTTTTGTTCTCTGGACAGTTCTGCTGACCTCATCCGCCATGTCTACTTCCACTGCTACCAC ACCAAGCTGAAACAGTGGGGGCTGCAGGCCTTGCAAAGCCAGGCTGACCTTGGCCCCTGCATCCTGGAC TTCCAGAGCCGGAACGTCATCCCTGATATCCCTGACCACTTCCTGTGTCTGTGGGAGCACTGTGAGAAT TCCTTCGACAATCCTGAGTGGTTTTATCGGCATGTGGAAGCACACAGTCTGTGCTGTGAATACGAAGCA GTCGGCAAGGACAACCCGGTGGTGCTGTGTGGCTGGAAAGGCTGTACCTGCACCTTCAAGGACCGCAGT AAACTTCGAGAGCACCTCCGCAGCCATACCCAGGAGAAAGTGGTAGCCTGCCCCACCTGTGGGGGCATG TTTGCCAACAATACCAAGTTCTTAGATCACATCCGTCGCCAGACCTCATTGGATCAGCAGCACTTCCAG TGTTCTCACTGTTCCAAGAGATTTGCCACAGAGCGGCTATTGCGGGACCACATGCGCAACCATGTGAAT CACTATAAGTGCCCTCTGTGTGACATGACCTGCCCGCTGCCTTCCTCCCTCCGCAACCACATGCGCTTT CGTCACAGTGAGGACCGGCCCTTTAAATGTGACTGTTGTGACTACAGCTGCAAGAATCTTATTGACCTC CAGAAGCACCTGGATACCCACAGCGAGGAGCCAGCCTACAGGTGTGATTTTGAGAACTGCACCTTCAGT GCCCGATCCCTCTGCTCTATCAAGTCCCATTACCGCAAAGTACATGAAGGAGACTCTGAGCCAAGGTAC AAATGTCATGTGTGTGACAAATGCTTCACACGGGGCAACAACCTCACCGTGCACCTTCGCAAGAAGCAC CAGTTCAAGTGGCCCTCAGGGCATCCCCGTTTTCGCCTCGGCCATTTCTGTATCCCCCTGCCTGGGTTT GCTGCCCTTTATGCTCCTACCTCACCAGGTACAAGGAACATGAAGATGGCTATATGCGGCTGCAGCTGG TTCGCTACGAGAGTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001243259
Insert Size	1260 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243259.1
RefSeq Size	2319 bp
RefSeq ORF	1260 bp
Locus ID	25988
UniProt ID	Q9BQA5
Protein Families	Transcription Factors
MW	49 kDa
Gene Summary	This gene encodes a transcription factor that interacts with methyl-CpG-binding protein-2 (MBD2), a component of the MeCP1 histone deacetylase (HDAC) complex, and plays a role in DNA methylation and transcription repression. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Aug 2011] Transcript Variant: This variant (3) uses an alternate acceptor splice site at the 3' terminal exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1.

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