DENND5A (NM_001243254) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DEE49; EIEE49; RAB6IP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331983 representing NM_001243254. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGTGGCGGCGGCGGCGGAGGGGGCTCGGCGCCCAGTCGCTTCGCCGACTACTTTGTCATCTGCGGA CTGGACACGGAGACCGGGCTGGAGCCGGACGAGCTGTCGGCATTATGCCAGTACATACAGGCTTCTAAA GCCAGGGATGGTGCCAGCCCTTTCATTTCAAGTACGACTGAAGGAGAAAATTTTGAGCAGACACCATTG AGAAGAACATTCAAATCTAAGGTCCTTGCACGATATCCTGAGAACGTAGAATGGAATCCCTTTGACCAA GATGCAGTAGGAATGCTATGTATGCCGAAAGGGCTGGCATTCAAGACCCAGGCTGATCCCAGGGAGCCC CAATTCCATGCCTTTATTATCACAAGGGAGGATGGCTCTCGGACATTTGGGTTTGCCCTCACATTTTAT GAAGAGGTGACTAGCAAGCAGATCTGCAGTGCAATGCAGACCCTCTACCACATGCACAATGCTGAGTAT GATGTCCTACATGCTCCCCCTGCTGATGACAGAGACCAGAGCAGCATGGAGGATGGTGAAGACACTCCT GTGACCAAACTGCAGCGCTTCAACTCCTATGACATTAGCCGGGACACTCTCTACGTCTCTAAGTGCATC TGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCTGGAGCAACTCCACCAGGCAGTC ACTTCACCTCAGCCCCCTCCACTGCCCCTTGAGAGCTACATATACAACGTACTCTACGAGGTGCCGCTC CCACCTCCTGGCCGGTCCTTGAAGTTTTCTGGGGTCTATGGGCCAATAATCTGCCAGAGACCAAGTACC AATGAGCTTCCCCTATTTGACTTTCCTGTCAAAGAGGTTTTTGAACTGCTCGGGGTGGAGAATGTGTTT CAGCTTTTTACTTGTGCCCTTCTGGAGTTTCAAATCCTGCTCTACTCACAGCATTACCAGAGACTGATG ACTGTGGCGGAGACGATTACAGCTCTCATGTTTCCTTTCCAGTGGCAGCATGTCTATGTCCCTATTCTC CCAGCTTCTCTCCTGCATTTCTTAGATGCTCCTGTTCCATACCTGATGGGTTTGCATTCCAATGGCCTG GATGACCGGTCAAAGCTGGAGCTGCCTCAAGAGGCTAACCTCTGCTTTGTGGACATTGACAACCACTTC ATTGAGTTGCCAGAGGACTTGCCACAGTTCCCCAACAAATTGGAGTTTGTCCAGGAAGTCTCTGAGATT CTCATGGCATTTGGAATTCCCCCTGAAGGGAATCTTCATTGCAGTGAGAGTGCCTCCAAGCTGAAGAGG CTGCGGGCCTCTGAGCTTGTCTCGGACAAGAGGAATGGGAACATTGCTGGCTCCCCTTTGCATTCCTAC GAGCTTCTTAAGGAGAATGAAACTATTGCCCGGCTGCAAGCCTTGGTCAAGAGAACTGGGGTGAGCCTG GAAAAGTTGGAAGTGCGTGAAGACCCCAGCAGCAATAAGGATCTCAAAGTTCAGTGTGATGAAGAAGAA CTCAGGATTTACCAGCTAAACATTCAGATCCGGGAAGTTTTTGCAAATCGTTTCACTCAGATGTTTGCA GATTATGAGGTGTTTGTCATCCAACCCAGCCAGGATAAGGAATCCTGGTTTACCAACAGGGAGCAAATG CAAAACTTTGATAAAGCATCTTTTCTGTCAGATCAGCCTGAGCCCTACCTGCCCTTCCTCTCAAGATTC CTGGAGACCCAGATGTTTGCATCTTTCATTGACAACAAAATAATGTGTCATGATGATGATGATAAAGAC CCTGTACTCCGGGTATTTGATTCCCGAGTTGACAAGATCAGGCTGTTGAATGTTCGGACACCTACTCTC CGTACATCCATGTACCAGAAGTGTACCACTGTGGATGAAGCAGAGAAAGCAATTGAGCTGCGTCTGGCA AAAATTGACCATACTGCAATTCACCCACATTTACTTGACATGAAGATTGGACAAGGGAAATATGAGCCG GGCTTCTTCCCTAAGCTGCAGTCTGATGTACTTTCCACTGGGCCAGCCAGCAACAAGTGGACGAAAAGG AATGCCCCTGCCCAGTGGAGGCGGAAAGATCGGCAGAAGCAGCACACAGAACACCTGCGTTTAGATAAT GACCAGAGGGAGAAGTACATCCAGGAAGCCAGGACTATGGGCAGCACTATCCGCCAGCCCAAACTGTCC AACCTCTCTCCATCAGTGATTGCCCAGACCAATTGGAAGTTTGTAGAGGGCCTGCTGAAGGAATGCCGC AATAAGACCAAGAGGATGCTGGTGGAAAAGATGGGCCGAGAAGCTGTGGAGCTAGGGCATGGGGAGGTG AACATCACAGGGGTGGAAGAGAACACCCTGATTGCCAGCCTTTGTGATCTCCTGGAAAGGATCTGGAGT CATGGACTACAAGTGAAACAGGGGAAATCAGCCTTATGGTCCCACCTGTTACATTATCAGGACAACCGG CAGAGAAAACTCACATCAGGAAGCCTCAGTACCTCAGGAATACTTCTTGATTCAGAACGTAGGAAGTCT GATGCCAGCTCACTCATGCCTCCCCTGAGGATCTCCCTGATTCAGGATATGAGGCACATCCAGAACATC GGGGAAATCAAGACTGATGTGGGAAAGGCCAGAGCATGGGTGCGACTGTCCATGGAAAAAAAGTTACTT TCCAGACACCTGAAGCAGCTCCTCTCAGACCATGAGCTCACCAAAAAGTTATATAAGCGCTATGCCTTC CTGCGCTGTGATGACGAGAAGGAGCAGTTCCTCTATCACCTCCTGTCTTTCAATGCCGTCGATTACTTT TGCTTCACCAATGTCTTCACAACTATCCTGATCCCGTACCACATTCTGATCGTACCAAGCAAGAAGCTG GGGGGCTCCATGTTCACTGCCAACCCATGGATCTGTATATCAGGAGAATTGGGTGAGACACAGATCATG CAGATTCCCAGGAATGTGCTAGAGATGACCTTCGAGTGCCAGAACTTGGGGAAGCTTACTACTGTCCAG ATTGGCCATGATAACTCTGGGCTGTATGCCAAATGGCTGGTGGAGTATGTGATGGTCAGGAATGAGATC ACAGGACATACCTACAAGTTCCCGTGTGGCCGGTGGTTAGGGAAGGGCATGGATGATGGAAGCCTGGAG CGGATCCTAGTTGGGGAGCTGCTCACATCCCAGCCTGAGGTGGATGAGAGGCCATGCCGGACCCCGCCG CTGCAGCAGTCCCCCAGTGTCATCCGGAGGCTTGTTACCATCTCACCCAACAACAAGCCCAAGCTGAAC ACTGGGCAGATCCAGGAGTCCATCGGGGAGGCAGTCAATGGCATTGTGAAGCACTTCCATAAGCCTGAG AAAGAGCGAGGCAGTCTGACGCTGTTGCTCTGTGGAGAGTGTGGCCTTGTCTCGGCCTTGGAACAGGCT TTCCAGCATGGATTTAAATCGCCCCGGCTCTTCAAAAATGTCTTCATTTGGGATTTCCTGGAAAAAGCA CAAACCTATTATGAGACATTAGAGAAGAATGAAGTAGTCCCTGAGGAAAACTGGCATACAAGAGCCCGG AACTTCTGCCGATTTGTCACTGCAATCAACAATACTCCCCGGAACATCGGCAAGGATGAGATCACCTCC TACACCACTGGATTGCCCTGCTGGCTGACTGCCCCATCACTGCACACATGTATGAGGATGTGGCACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243254
Insert Size	3726 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243254.1
RefSeq Size	4945 bp
RefSeq ORF	3726 bp
Locus ID	23258
UniProt ID	Q6IQ26
MW	142 kDa
Gene Summary	This gene encodes a DENN-domain-containing protein that functions as a RAB-activating guanine nucleotide exchange factor (GEF). This protein catalyzes the conversion of GDP to GTP and thereby converts inactive GDP-bound Rab proteins into their active GTP-bound form. The encoded protein is recruited by RAB6 onto Golgi membranes and is therefore referred to as RAB6-interacting protein 1. This protein binds with RAB39 as well. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene are associated with early infantile epileptic encephalopathy-49. [provided by RefSeq, Feb 2017] Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1.

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