IF3EI (EIF3L) (NM_001242923) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EIF3EIP; EIF3S6IP; EIF3S11; HSPC021; HSPC025; MSTP005
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331928 representing NM_001242923. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTTATCCCGCTGATGATTATGAGTCTGAGGCGGCTTATGACCCCTACGCTTATCCCAGCGACTAT GATATGCACACAGGAGATCCAAAGCAGGACCTTGCTTATGAACGTCAGTATGAACAGCAAACCTATCAG GTGATCCCTGAGGTGATCAAAAACTTCATCCAGTATTTCCACAAAACTGTCTCAGATTTGATTGACCAG AAAGTGTATGAGCTACAGGCCAGTCGTGTCTCCAGTGATGTCATTGACCAGAAGGTGTATGAGATCCAG GACATCTATGAGAACAGCTGGACCAAGCTGACTGAAAGATTCTTCAAGAATACACCTTGGCCCGAGGCT GAAGCCATTGCTCCACAGGTTGGCAATGATGCTGTCTTCCTGATTTTATACAAAGAATTATACTACAGG CACATATATGCCAAAGTCAGTTTTCAGTCATTCAGTCAGTACCGCTGTAAGACTGCCAAGAAGTCAGAG GAGGAGATTGACTTTCTTCGTTCCAATCCCAAAATCTGGAATGTTCATAGTGTCCTCAATGTCCTTCAT TCCCTGGTAGACAAATCCAACATCAACCGACAGTTGGAGGTATACACAAGCGGAGGTGACCCTGAGAGT GTGGCTGGGGAGTATGGGCGGCACTCCCTCTACAAAATGCTTGGTTACTTCAGCCTGGTCGGGCTTCTC CGCCTGCACTCCCTGTTAGGAGATTACTACCAGGCCATCAAGGTGCTGGAGAACATCGAACTGAACAAG AAGAGTATGTATTCCCGTGTGCCAGAGTGCCAGGTCACCACATACTATTATGTTGGGTTTGCATATTTG ATGATGCGTCGTTACCAGGATGCCATCCGGGTCTTCGCCAACATCCTCCTCTACATCCAGAGGACCAAG AGCATGTTCCAGAGGACCACGTACAAGTATGAGATGATTAACAAGCAGAATGAGCAGATGCATGCGCTG CTGGCCATTGCCCTCACGATGTACCCCATGCGTATTGATGAGAGCATTCACCTCCAGCTGCGGGAGAAA TATGGGGACAAGATGTTGCGCATGCAGAAAGGTGACCCACAAGTCTATGAAGAACTTTTCAGTTACTCC TGCCCCAAGTTCCTGTCGCCTGTAGTGCCCAACTATGATAATGTGCACCCCAACTACCACAAAGAGCCC TTCCTGCAGCAGCTGAAGGTGTTTTCTGATGAAGTACAGCAGCAGGCCCAGCTTTCAACCATCCGCAGC TTCCTGAAGCTCTACACCACCATGCCTGTGGCCAAGCTGGCTGGCTTCCTGGACCTCACAGAGCAGGAG TTCCGGATCCAGCTTCTTGTCTTCAAACACAAGATGAAGAACCTCGTGTGGACCAGCGGTATCTCAGCC CTGGATGGTGAATTTCAGTCAGCCTCAGAGGTTGACTTCTACATTGATAAGGACATGATCCACATCGCG GACACCAAGGTCGCCAGGCGTTATGGGGATTTCTTCATCCGTCAGATCCACAAATTTGAGGAGCTTAAT CGAACCCTGAAGAAGATGGGACAGAGACCTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242923
Insert Size	1551 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242923.1
RefSeq Size	1947 bp
RefSeq ORF	1551 bp
Locus ID	51386
UniProt ID	Q9Y262
MW	61 kDa
Gene Summary	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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