GRB7 (NM_001242442) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331802 representing NM_001242442. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGAAAGTGGAGGCCGGGACAAGGGCACACAACTGGTTCCGTTAAGCCCCTCTCTCGCTCAGACGCC ATGGAGCTGGATCTGTCTCCACCTCATCTTAGCAGCTCTCCGGAAGACCTTTGCCCAGCCCCTGGGACC CCTCCTGGGACTCCCCGGCCCCCTGATACCCCTCTGCCTGAGGAGGTAAAGAGGTCCCAGCCTCTCCTC ATCCCAACCACCGGCAGGAAACTTCGAGAGGAGGAGAGGCGTGCCACCTCCCTCCCCTCTATCCCCAAC CCCTTCCCTGAGCTCTGCAGTCCTCCCTCACAGAGCCCAATTCTCGGGGGCCCCTCCAGTGCAAGGGGG CTGCTCCCCCGCGATGCCAGCCGCCCCCATGTAGTAAAGGTGTACAGTGAGGATGGGGCCTGCAGGTCT GTGGAGGTGGCAGCAGGTGCCACAGCTCGCCACGTGTGTGAAATGCTGGTGCAGCGAGCTCACGCCTTG AGCGACGAGACCTGGGGGCTGGTGGAGTGCCACCCCCACCTAGCACTGGAGCGGGGTTTGGAGGACCAC GAGTCCGTGGTGGAAGTGCAGGCTGCCTGGCCCGTGGGCGGAGATAGCCGCTTCGTCTTCCGGAAAAAC TTCGCCAAGTACGAACTGTTCAAGAGCTCCCCACACTCCCTGTTCCCAGAAAAAATGGTCTCCAGCTGT CTCGATGCACACACTGGTATATCCCATGAAGACCTCATCCAGAACTTCCTGAATGCTGGCAGCTTTCCT GAGATCCAGGGCTTTCTGCAGCTGCGGGGTTCAGGACGGAAGCTTTGGAAACGCTTTTTCTGCTTCTTG CGCCGATCTGGCCTCTATTACTCCACCAAGGGCACCTCTAAGGATCCGAGGCACCTGCAGTACGTGGCA GATGTGAACGAGTCCAACGTGTACGTGGTGACGCAGGGCCGCAAGCTCTACGGGATGCCCACTGACTTC GGTTTCTGTGTCAAGCCCAACAAGCTTCGAAATGGCCACAAGGGGCTTCGGATCTTCTGCAGTGAAGAT GAGCAGAGCCGCACCTGCTGGCTGGCTGCCTTCCGCCTCTTCAAGTACGGGGTGCAGCTGTACAAGAAT TACCAGCAGGCACAGTCTCGCCATCTGCATCCATCTTGTTTGGGCTCCCCACCCTTGAGAAGTGCCTCA GATAATACCCTGGTGGCCATGGACTTCTCTGGCCATGCTGGGCGTGTCATTGAGAACCCCCGGGAGGCT CTGAGTGTGGCCCTGGAGGAGGCCCAGGCCTGGAGGAAGAAGACAAACCACCGCCTCAGCCTGCCCATG CCAGCCTCCGGCACGAGCCTCAGTGCAGCCATCCACCGCACCCAACTCTGGTTCCACGGGCGCATTTCC CGTGAGGAGAGCCAGCGGCTTATTGGACAGCAGGGCTTGGTAGACGGCCTGTTCCTGGTCCGGGAGAGT CAGCGGAACCCCCAGGGCTTTGTCCTCTCTTTGTGCCACCTGCAGAAAGTGAAGCATTATCTCATCCTG CCGAGCGAGGAGGAGGGCCGCCTGTACTTCAGCATGGATGATGGCCAGACCCGCTTCACTGACCTGCTG CAGCTCGTGGAGTTCCACCAGCTGAACCGCGGCATCCTGCCGTGCTTGCTGCGCCATTGCTGCACGCGG GTGGCCCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242442
Insert Size	1668 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242442.1
RefSeq Size	2214 bp
RefSeq ORF	1668 bp
Locus ID	2886
UniProt ID	Q14451
Protein Families	Druggable Genome, Embryonic stem cells, Stem cell - Pluripotency
MW	62.1 kDa
Gene Summary	The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (4) encodes the longest isoform (b).

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