TGN46 (TGOLN2) (NM_001206841) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hTGN46; hTGN48; hTGN51; TGN38; TGN46; TGN48; TGN51; TTGN2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331692 representing NM_001206841. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCGGTTCGTGGTTGCCTTGGTCCTCCTGAACGTCGCAGCGGCGGGAGCCGTGCCGCTCTTGGCCACC GAAAGCGTCAAGCAAGAAGAAGCTGGAGTACGGCCTTCTGCAGGAAACGTCTCCACCCACCCCAGCTTG AGCCAACGGCCTGGAGGCTCTACCAAGTCGCATCCGGAGCCGCAGACTCCAAAAGACAGCCCTAGCAAG TCGAGTGCGGAGGCGCAGACCCCAGAAGACACCCCCAACAAGTCGGGTGCGGAGGCAAAGACCCAAAAA GACAGCTCCAACAAGTCGGGTGCGGAGGCAAAGACCCAAAAAGGCAGCACTAGCAAGTCGGGTTCGGAG GCGCAGACCACAAAAGACAGCACTAGTAAGTCGCATCCGGAGCTGCAGACTCCAAAAGACAGCACTGGC AAATCGGGTGCGGAGGCGCAGACCCCAGAAGACAGCCCCAACAGGTCGGGTGCGGAGGCAAAGACCCAA AAAGACAGCCCTAGCAAGTCAGGTTCGGAGGCGCAGACCACAAAAGATGTCCCTAATAAGTCGGGTGCG GACGGCCAGACCCCAAAAGACGGCTCCAGCAAGTCGGGTGCGGAGGATCAGACCCCAAAAGACGTCCCT AACAAGTCGGGTGCGGAGAAGCAGACTCCAAAAGACGGCTCTAACAAGTCCGGTGCAGAGGAGCAGGGC CCAATAGACGGGCCCAGCAAGTCGGGTGCGGAGGAGCAGACCTCAAAAGACAGCCCTAACAAGGTGGTT CCAGAGCAGCCTTCCCGGAAAGACCATTCCAAGCCCATCTCCAACCCTTCTGATAACAAGGAGCTCCCC AAGGCTGACACAAACCAGCTTGCTGACAAAGGGAAGCTTTCTCCTCATGCTTTCAAAACCGAATCTGGG GAGGAAACTGACCTCATTTCTCCCCCGCAGGAGGAAGTTAAGTCTTCAGAGCCTACTGAGGATGTGGAG CCCAAAGAGGCTGAAGATGATGATACAGGACCCGAGGAGGGCTCACCGCCCAAAGAAGAGAAAGAAAAG ATGTCCGGTTCTGCCTCCAGTGAGAACCGTGAAGGGACACTTTCGGATTCCACGGGTAGCGAGAAGGAT GACCTTTATCCGAACGGTTCTGGAAATGGCAGCGCGGAGAGCAGCCACTTCTTTGCATATCTGGTGACT GCAGCCATTCTTGTGGCTGTCCTCTATATCGCTCATCACAACAAGCGGAAGATCATTGCTTTTGTCCTG GAAGGAAAAAGATCTAAAGTCACCCGGCGGCCAAAGGCCAGTGACTACCAACGTTTGGACCAGAAGATC TTTTTTCCCCCAAGTCCTAACAGAATGGTATATTCCTCTGGAAAAAGATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001206841
Insert Size	1362 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206841.1
RefSeq Size	6390 bp
RefSeq ORF	1362 bp
Locus ID	10618
UniProt ID	O43493
Protein Families	Transmembrane
MW	47.8 kDa
Gene Summary	This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network, a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network, and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (3, A allele) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) has a longer and distinct C-terminus, compared to isoform 1. This variant (3, A allele) differs at 1 nt position compared to variant 3, B allele. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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