KIAA1432 (RIC1) (NM_001206557) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA207C16.1; CATIFA; CIP150; KIAA1432
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331656 representing NM_001206557. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTATTTTCTGAGCGGCTGGCCCAAGAGGCTGCTGTGCCCTCTGGGGAGCCCGGCCGAGGCGCCTTTC CACGTTCAGTCCGACCCGCAGAGGGCTTTCTTCGCCGTGCTGGCCGCGGCCCGCCTCAGCATCTGGTAC AGCCGACCTAGTGTGTTAATTGTAACCTACAAGGAGCCTGCAAAATCATCTACTCAGTTTGGATCCTAC AAGCAAGCTGAATGGAGGCCAGATAGTACCATGATAGCTGTATCAACGGCAAATGGATACATCTTGTTT TTTCATATTACATCTACAAGAGGGGACAAGTACCTTTATGAACCAGTGTATCCCAAAGGAAGTCCACAA ATGAAGGGGACACCCCATTTTAAGGAAGAACAGTGTGCTCCAGCATTAAATTTGGAGATGAGGAAAATA CTGGATTTACAAGCACCCATCATGAGTTTGCAGTCTGTGTTGGAAGATCTCCTGGTTGCTACTTCTGAT GGACTTCTTCATCTTATTCACTGGGAAGGAATGACAAATGGAAGGAAAGCCATTAATCTTTGCACAGTA CCCTTTTCAGTAGACCTGCAGTCATCTAGAGTAGGTTCATTCCTGGGCTTCACAGACGTACACATCAGA GACATGGAATACTGTGCCACACTTGATGGGTTTGCTGTTGTATTTAATGATGGTAAAGTTGGATTTATT ACACCAGTGTCAAGTAGATTTACTGCAGAGCAGCTTCATGGAGTTTGGCCACAAGATGTTGTTGACGGA ACGTGTGTAGCAGTAAATAACAAGTATCGACTAATGGCATTTGGCTGTGTGAGTGGTTCTGTGCAGGTC TATACAATAGATAACAGCACTGGAGCCATGCTGCTATCTCATAAATTAGAGCTAACAGCAAAACAGTAT CCTGACATTTGGAATAAAACAGGAGCTGTTAAATTGATGAGATGGTCTCCTGACAATAGTGTTGTAATA GTGACCTGGGAATACGGAGGCCTTTCTTTATGGAGTGTTTTTGGAGCACAGCTGATTTGTACACTTGGA GGAGATTTTGCTTATAGGTCTGATGGCACCAAAAAAGATCCCCTTAAGATCAACTCTATGAGCTGGGGT GCAGAAGGCTATCACCTATGGGTAATCAGCGGATTTGGTTCTCAAAACACTGAAATTGAGTCTGACCTC AGGAGTGTAGTTAAACAGCCCAGCATCCTGTTATTTCAGTTTATTAAGAGTGTACTCACTGTAAACCCT TGTATGAGTAACCAAGAGCAGGTGTTGCTTCAGGGTGAGGATCGCTTGTACTTGAACTGTGGAGAGGCT TCACAAACCCAGAATCCCAGGAGTTCTTCAACACACTCTGAGCATAAGCCCAGTCGAGAAAAGAGCCCA TTTGCAGATGGAGGTTTAGAGTCTCAGGGATTAAGCACTTTACTTGGACATCGGCATTGGCATGTTGTA CAGATTTCCAGCACCTATCTAGAGAGCAATTGGCCTATACGGGAGCAAAATATGATCGTGACAGGTGGC TTAGCCTGGTGGAATGATTTTATGGTCCTTGCGTGTTATAACATAAATGACCGTCAAGAAGAGCTTAGA GTATACTTGCGAACATCAAATCTGGACAATGCCTTTGCTCATGTCACCAAAGCACAAGCAGAAACATTA CTGCTTAGTGTCTTCCAGGACATGGTAATAGTATTTAGAGCAGACTGTTCAATATGCCTTTACAGTATT GAAAGAAAATCTGATGGTCCAAATACTACTGCTGGTATTCAAGTTCTTCAGGAGGTTTCCATGTCACGC TACATTCCTCACCCTTTCCTGGTGGTATCTGTCACTCTGACATCAGTGAGTACAGAGAATGGAATCACC TTGAAAATGCCACAGCAGGCTCGTGGTGCAGAGAGCATTATGTTAAACCTGGCAGGACAGCTCATCATG ATGCAGAGGGACAGGTCAGGCCCACAGATCCGGGAGAAGGACAGTAACCCTAATAACCAAAGGAAACTT CTGCCATTCTGTCCTCCTGTTGTACTAGCCCAGTCTGTTGAAAATGTCTGGACAACGTGTCGAGCAAAT AAACAGAAACGTCACCTTCTGGAGGCCCTCTGGCTGAGCTGTGGTGGTGCAGGGATGAAAGTTTGGCTC CCTCTCTTCCCTAGGGATCACCGCAAGCCCCATTCCTTCTTGTCCCAGCGGATCATGCTGCCTTTCCAC ATCAACATTTACCCGCTAGCTGTTCTGTTTGAAGATGCTTTAGTCCTTGGTGCTGTCAATGACACTTTG CTCTATGATTCTTTATATACTCGGAACAATGCTAGAGAACAGCTGGAGGTGCTCTTCCCTTTCTGTGTT GTGGAGAGAACCTCTCAGATCTACCTCCACCACATTCTACGTCAACTTCTGGTCAGAAACCTTGGGGAG CAAGCCTTGCTCTTGGCCCAGTCCTGTGCCACATTACCTTACTTCCCTCATGTGCTGGAGCTCATGCTC CATGAAGTACTGGAAGAAGAAGCTACCTCACGGGAGCCCATTCCCGACCCTCTGCTTCCCACTGTGGCA AAATTTATCACTGAGTTCCCCCTCTTCCTGCAGACAGTTGTCCATTGTGCCAGGAAGACCGAATATGCC CTGTGGAATTACCTTTTTGCAGCTGTTGGAAACCCTAAGGACTTGTTTGAGGAGTGTTTGATGGCTCAG GATTTGGACACAGCTGCCTCTTACCTTATTATCTTACAGAATATGGAAGTCCCTGCAGTAAGTAGGCAA CATGCTACCCTTCTATTCAACACAGCACTAGAACAAGGCAAGTGGGACCTTTGTCGACACATGATTCGA TTTCTTAAAGCCATTGGCTCTGGAGAATCTGAGACACCTCCATCCACACCCACAGCTCAGGAACCCAGT TCAAGTGGTGGATTTGAGTTCTTCAGGAATCGAAGCATCAGTTTATCCCAGTCAGCTGAAAATGTTCCT GCCAGTAAATTCAGTTTACAGAAAACACTAAGTATGCCATCTGGTCCCTCTGGAAAAAGATGGAGCAAA GACAGTGACTGTGCTGAGAACATGTATATTGACATGATGCTCTGGAGACATGCTCGGCGCCTCTTAGAA GATGTGAGGTTAAAGGACCTTGGCTGCTTTGCAGCCCAGCTGGGCTTTGAACTAATTAGTTGGCTATGC AAGGAACGTACCCGAGCCGCCCGGGTAGACAACTTTGTAATAGCCCTGAAGAGACTCCACAAAGATTTC CTGTGGCCACTTCCAATCATCCCAGCCTCTTCTATCAGTTCTCCTTTCAAAAATGGAAAATACCGAACT GTGGGAGAGCAGCTGTTAAAGTCTCAATCAGCTGACCCATTTTTGAACCTTGAGATGGATGCTGGCATC TCCAACATCCAGCGAAGTCAGAGCTGGCTCAGCAACATTGGCCCCACCCATCATGAGATAGACACAGCT TCATCCCATGGACCACAAATGCAAGATGCCTTCTTGTCACCTTTATCTAATAAAGGTGATGAATGCAGT ATTGGTTCAGCCACAGACTTGACTGAAAGTAGCTCCATGGTGGATGGCGACTGGACAATGGTGGATGAA AATTTCTCTACACTCAGTTTAACTCAGTCAGAGCTGGAGCACATTTCCATGGAGTTGGCCAGTAAAGGG CCTCATAAATCCCAGGTCCAGCTTCGGTATTTGCTACACATTTTCATGGAGGCAGGGTGCCTAGACTGG TGCATCGTTATAGGCCTGATTCTTAGAGAATCCTCAATAATCAATCAGATTTTGGTTATTACACAGTCT TCAGAGGTAGATGGAGAGATGTTACAGAACATAAAGACAGGGCTCCATGCAGTGGACCGATGGGCCTCT ACAGACTGTCCTGGATATAAGCCATTTTTAAACATCATTAAGCCACAACTGCAGAAGCTCAGTGAGATA ACAGAAGAGCAGGTCCAGCCAGATGCCTTCCAACCAATAACTATGGGTAAGACTCCAGAACAGACTAGC CCCCGGGCAGAGGAGAGCAGGGGCTCCTCCAGCCATGGAAGCATCCCCCAGGGTGAAGTTGGAAGCAGC AATATGGTCAGCCGGAAAGAGGAGGACACAGCCCAAGCAGAGGAGGAAGAACCTTTTCAGGATGGGACT TACGACTGTTCTGTGTCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001206557
Insert Size	4161 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206557.1
RefSeq Size	6676 bp
RefSeq ORF	4161 bp
Locus ID	57589
UniProt ID	Q4ADV7
MW	155.2 kDa
Gene Summary	The RIC1-RGP1 complex acts as a guanine nucleotide exchange factor (GEF), which activates RAB6A by exchanging bound GDP for free GTP and may thereby required for efficient fusion of endosome-derived vesicles with the Golgi compartment. The RIC1-RGP1 complex participates in the recycling of mannose-6-phosphate receptors. Required for phosphorylation and localization of GJA1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks and in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform c), compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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