SPHK2 (NM_001204158) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SK-2; SK 2; SPK-2; SPK 2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331509 representing NM_001204158. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGGCCCTGCACATACAGCGGCTGCGCCCCAAA CCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGTCTCAGGCTGCTGCACCCTGCGAAGC CGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCATCTACACCTACCCTCGGGGCCGGCGCGGGGCCCGG CGCAGAGCCACTCGCACCTTCCGGGCAGATGGGGCCGCCACCTACGAAGAGAACCGTGCCGAGGCCCAG CGCTGGGCCACTGCCCTCACCTGTCTGCTCCGAGGACTGCCACTGCCCGGGGATGGGGAGATCACCCCT GACCTGCTACCTCGGCCGCCCCGGTTGCTTCTATTGGTCAATCCCTTTGGGGGTCGGGGCCTGGCCTGG CAGTGGTGTAAGAACCACGTGCTTCCCATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATCCAGACA GAACGACAGAACCACGCCCGGGAGCTGGTCCAGGGGCTGAGCCTGAGTGAGTGGGATGGCATCGTCACG GTCTCGGGAGACGGGCTGCTCCATGAGGTGCTGAACGGGCTCCTAGATCGCCCTGACTGGGAGGAAGCT GTGAAGATGCCTGTGGGCATCCTCCCCTGCGGCTCGGGCAACGCGCTGGCCGGAGCAGTGAACCAGCAC GGGGGATTTGAGCCAGCCCTGGGCCTCGACCTGTTGCTCAACTGCTCACTGTTGCTGTGCCGGGGTGGT GGCCACCCACTGGACCTGCTCTCCGTGACGCTGGCCTCGGGCTCCCGCTGTTTCTCCTTCCTGTCTGTG GCCTGGGGCTTCGTGTCAGATGTGGATATCCAGAGCGAGCGCTTCAGGGCCTTGGGCAGTGCCCGCTTC ACACTGGGCACGGTGCTGGGCCTCGCCACACTGCACACCTACCGCGGACGCCTCTCCTACCTCCCCGCC ACTGTGGAACCTGCCTCGCCCACCCCTGCCCATAGCCTGCCTCGTGCCAAGTCGGAGCTGACCCTAACC CCAGACCCAGCCCCGCCCATGGCCCACTCACCCCTGCATCGTTCTGTGTCTGACCTGCCTCTTCCCCTG CCCCAGCCTGCCCTGGCCTCTCCTGGCTCGCCAGAACCCCTGCCCATCCTGTCCCTCAACGGTGGGGGC CCAGAGCTGGCTGGGGACTGGGGTGGGGCTGGGGATGCTCCGCTGTCCCCGGACCCACTGCTGTCTTCA CCTCCTGGCTCTCCCAAGGCAGCTCTACACTCACCCGTCTCCGAAGGGGCCCCCGTAATTCCCCCATCC TCTGGGCTCCCACTTCCCACCCCTGATGCCCGGGTAGGGGCCTCCACCTGCGGCCCGCCCGACCACCTG CTGCCTCCGCTGGGCACCCCGCTGCCCCCAGACTGGGTGACGCTGGAGGGGGACTTTGTGCTCATGTTG GCCATCTCGCCCAGCCACCTAGGCGCTGACCTGGTGGCAGCTCCGCATGCGCGCTTCGACGACGGCCTG GTGCACCTGTGCTGGGTGCGTAGCGGCATCTCGCGGGCTGCGCTGCTGCGCCTTTTCTTGGCCATGGAG CGTGGTAGCCACTTCAGCCTGGGCTGTCCGCAGCTGGGCTACGCCGCGGCCCGTGCCTTCCGCCTAGAG CCGCTCACACCACGCGGCGTGCTCACAGTGGACGGGGAGCAGGTGGAGTATGGGCCGCTACAGGCACAG ATGCACCCTGGCATCGGTACACTGCTCACTGGGCCTCCTGGCTGCCCGGGGCGGGAGCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204158
Insert Size	1788 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204158.2
RefSeq Size	2787 bp
RefSeq ORF	1788 bp
Locus ID	56848
UniProt ID	Q9NRA0
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
MW	63 kDa
Gene Summary	This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a.

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