ASB3 (NM_001201965) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASB-3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331446 representing NM_001201965. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGACCTTTGAAGGTTTCTGTGCTTTGCATCTCGCTGCAAGTCAAGGACATTGGAAAATCGTACAG ATTCTTTTAGAAGCTGGGGCAGATCCTAATGCAACTACTTTAGAAGAAACGACACCATTGTTTTTAGCT GTTGAAAATGGACAGATAGATGTGTTAAGGCTGTTGCTTCAACACGGAGCAAATGTTAATGGATCCCAT TCTATGTGTGGATGGAACTCCTTGCACCAGGCTTCTTTTCAGGAAAATGCTGAGATCATAAAATTGCTT CTTAGAAAAGGAGCAAACAAGGAATGCCAGGATGACTTTGGAATCACACCTTTATTTGTGGCTGCTCAG TATGGCAAGCTAGAAAGCTTGAGCATACTTATTTCATCGGGTGCAAATGTCAATTGTCAAGCCTTGGAC AAAGCTACACCCTTGTTCATTGCTGCTCAAGAGGGACACACAAAATGTGTGGAGCTTTTGCTCTCCAGT GGGGCAGATCCTGATCTTTACTGTAATGAGGACAGTTGGCAGTTACCTATTCATGCAGCTGCACAAATG GGCCATACAAAAATCTTGGACTTGTTAATACCACTTACTAACCGGGCCTGTGACACTGGGCTAAACAAA GTAAGCCCTGTTTACTCAGCAGTGTTTGGGGGACATGAAGATTGCCTAGAAATATTACTCCGGAATGGC TACAGCCCAGACGCCCAGGCGTGCCTTGTTTTTGGATTCAGTTCTCCTGTGTGCATGGCTTTCCAAAAG GACTGTGAGTTCTTTGGAATTGTGAACATTCTTTTGAAATATGGAGCCCAGATAAATGAACTTCATTTG GCATACTGCCTGAAGTACGAGAAGTTTTCGATATTTCGCTACTTTTTGAGGAAAGGTTGCTCATTGGGA CCATGGAACCATATATATGAATTTGTAAATCATGCAATTAAAGCACAAGCAAAATATAAGGAGTGGTTG CCACATCTTCTGGTTGCTGGATTTGACCCACTGATTCTACTGTGCAATTCTTGGATTGACTCAGTCAGC ATTGACACCCTTATCTTCACTTTGGAGTTTACTAATTGGAAGACACTTGCACCAGCTGTTGAAAGGATG CTCTCTGCTCGTGCCTCAAACGCTTGGATTCTACAGCAACATATTGCCACTGTTCCATCCCTGACCCAT CTTTGTCGTTTGGAAATTCGGTCCAGTCTAAAATCAGAACGTCTACGGTCTGACAGTTATATTAGTCAG CTGCCACTTCCCAGAAGCCTACATAATTATTTGCTCTATGAAGACGTTCTGAGGATGTATGAAGTTCCA GAACTGGCAGCTATTCAAGATGGATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001201965
Insert Size	1338 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201965.1
RefSeq Size	2456 bp
RefSeq ORF	1338 bp
Locus ID	51130
UniProt ID	Q9Y575
Protein Families	Druggable Genome
MW	49.6 kDa
Gene Summary	The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2011] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Variants 2 and 3 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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