GBF1 (NM_001199379) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARF1GEF
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331301 representing NM_001199379. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGGATAAGAATATTTACATCATTCAAGGGGAGATTAACATTGTGGTTGGGGCCATCAAACGAAAT GCCCGATGGAGCACCCATACACCACTGGATGAAGAACGGGATCCTCTGCTGCATAGTTTCGGTCATCTA AAGGAGGTTTTAAACAGTATAACAGAACTCTCAGAAATTGAGCCCAATGTATTCCTTCGACCTTTTCTG GAAGTGATTCGCTCTGAAGATACCACTGGCCCTATCACTGGACTGGCACTCACCTCTGTCAACAAGTTC CTGTCCTATGCACTCATAGATCCCACCCATGAGGGCACAGCAGAGGGCATGGAGAACATGGCAGATGCT GTCACCCATGCTCGTTTTGTGGGCACGGATCCTGCCAGTGATGAAGTTGTCCTGATGAAAATCCTTCAG GTTCTACGGACTCTGCTGCTAACCCCAGTGGGTGCCCACCTAACCAATGAATCTGTGTGTGAGATTATG CAGTCTTGCTTCCGGATCTGCTTTGAAATGAGGCTCAGTGAGTTATTGAGAAAATCCGCAGAGCACACT CTCGTAGACATGGTGCAGCTGCTCTTCACAAGGTTACCTCAGTTTAAAGAAGAACCCAAGAACTATGTG GGGACCAACATGAAGAAGCTGAAAATGAGAGCCGGAGGCATGAGTGATTCATCCAAATGGAAGAAACAG AAGAGATCCCCTCGGCCCCCACGCCATATGACCAAAGTCACACCAGGTTCAGAGCTGCCCACTCCCAAT GGAACCACCTTATCATCTAACCTCACTGGTGGCATGCCCTTCATTGATGTGCCCACTCCCATCTCCTCT GCAAGTTCAGAAGCTGCCTCAGCAGTGGTCAGTCCCTCTACAGACAGTGGCCTGGAATTCTCCTCCCAA ACCACTTCCAAGGAAGACCTTACTGATCTAGAGCAACCTGGCTCTCCAGGGTACAGCACAGCTACAGAG CCTGGAAGCAGTGAGCTAGGTGTTCCCGAGCAGCCTGACCTCCAGGAAGGGACCCATGTGGAAAAGTCC CAGTCAGCATCTGTGGAGTCCATCCCTGAAGTGTTAGAGGAGTGCACGTCCCCTGCCGACCACTCTGAC TCTGCCTCTGTCCATGACATGGATTACGTCAATCCCCGGGGCGTGCGCTTTACACAGTCCTCCCAGAAA GAAGGCACAGCTTTGGTCCCCTATGGTCTTCCCTGCATCCGCGAGCTCTTCCGCTTCCTCATCTCCCTC ACCAATCCACACGACCGCCATAACTCAGAGGTTATGATTCACATGGGACTGCATTTGCTGACAGTGGCC CTTGAGTCAGCCCCTGTAGCCCAGTGCCAGACCCTCTTGGGCCTCATCAAGGATGAGATGTGCCGTCAC TTATTCCAGCTACTCAGCATAGAGCGACTAAACCTTTATGCTGCTTCCCTGCGAGTATGCTTCCTACTG TTTGAGAGCATGCGAGAGCACCTCAAGTTCCAAATGGAGATGTACATCAAAAAGCTTATGGAGATCATC ACTGTGGAGAACCCCAAGATGCCTTATGAGATGAAGGAGATGGCACTGGAGGCCATTGTGCAGCTCTGG CGCATCCCCAGCTTTGTCACAGAGCTCTACATCAACTATGATTGTGACTACTACTGTTCCAACCTCTTT GAGGAACTCACAAAGCTGCTGTCCAAGAATGCCTTCCCTGTGTCTGGTCAACTCTATACAACACACCTA CTATCTCTTGATGCCCTATTGACAGTGATTGACAGCACCGAGGCCCACTGCCAGGCTAAAGTCCTCAAC AGCCTCACCCAGCAAGAGAAGAAGGAGACAGCCAGACCAAGCTGTGAGATAGTAGATGGCACCCGAGAA GCTAGCAATACTGAGAGAACTGCCAGCGATGGGAAAGCTGTAGGCATGGCCTCAGACATCCCAGGCCTG CATCTGCCAGGTGGAGGGCGGCTGCCACCAGAACATGGGAAATCAGGATGCAGTGATCTGGAGGAAGCT GTTGACTCTGGGGCTGACAAAAAGTTTGCCCGGAAGCCACCCCGATTTTCCTGTCTCCTGCCAGATCCA CGGGAACTAATTGAAATTAAAAACAAAAAGAAGCTGCTAATCACTGGCACAGAGCAGTTCAATCAGAAA CCAAAGAAGGGGATTCAGTTTCTGCAAGAGAAAGGCCTCCTCACCATCCCAATGGACAACACAGAGGTT GCTCAGTGGCTCCGAGAGAACCCTCGGCTGGACAAGAAGATGATTGGAGAGTTTGTGAGTGACCGCAAA AACATTGACCTGTTGGAGAGCTTTGTGAGCACCTTCAGTTTTCAGGGTCTGCGACTGGACGAAGCCCTC CGCCTCTACCTGGAAGCCTTCCGTTTGCCTGGGGAAGCACCAGTCATCCAGAGGTTGCTGGAGGCATTC ACAGAGCGTTGGATGAATTGTAATGGCTCCCCATTTGCCAATAGCGATGCCTGCTTTTCCCTGGCCTAT GCTGTCATCATGCTTAATACTGACCAGCACAACCACAATGTTCGTAAACAGAATGCACCCATGACCCTG GAGGAGTTTCGCAAAAATCTGAAAGGTGTGAATGGAGGCAAGGACTTTGAGCAAGACATCCTGGAGGAC ATGTACCATGCCATCAAGAATGAGGAAATTGTAATGCCTGAGGAGCAGACAGGCTTGGTTCGGGAGAAC TATGTGTGGAATGTGCTGCTTCATCGAGGTGCCACCCCTGAGGGCATATTCCTGCGTGTGCCTACTGCC AGCTATGATCTTGACCTCTTCACCATGACCTGGGGCCCCACTATTGCTGCTCTCTCTTATGTCTTTGAC AAAAGCCTTGAGGAGACAATCATCCAGAAAGCCATCTCAGGCTTCAGGAAGTGCGCCATGATCTCCGCC CACTATGGCCTCAGCGATGTGTTTGACAATCTCATCATCTCTCTATGCAAATTCACAGCTCTCAGCAGT GAGTCTATTGAGAACCTGCCCAGTGTATTTGGAAGCAACCCTAAAGCCCATATTGCAGCCAAGACAGTA TTCCATTTGGCCCATCGTCATGGTGACATCCTGCGGGAGGGCTGGAAGAATATCATGGAGGCCATGCTG CAGCTCTTCCGAGCCCAACTACTGCCCAAGGCTATGATAGAGGTAGAAGATTTCGTGGATCCCAATGGC AAGATCTCTCTACAGCGGGAAGAGACACCATCAAACCGAGGAGAGTCAACAGTGCTGAGCTTTGTGAGC TGGCTAACACTGAGTGGTCCTGAGCAGTCTAGTGTTCGGGGCCCATCCACTGAAAACCAAGAGGCCAAG AGAGTGGCCTTAGAGTGTATAAAGCAATGTGACCCAGAAAAAATGATCACAGAAAGCAAGTTCCTCCAG CTGGAGTCACTACAGGAGCTCATGAAGGCTCTGGTCTCAGTGACACCAGATGAAGAGACATATGATGAG GAAGATGCTGCTTTCTGCCTAGAGATGCTGCTAAGGATTGTGTTGGAGAACAGGGATCGTGTGGGCTGT GTGTGGCAGACTGTTCGAGACCATCTATACCACCTCTGTGTTCAGGCACAAGATTTCTGCTTCCTTGTG GAGCGGGCAGTGGTGGGGTTGCTACGCCTGGCCATTCGGCTTCTCCGGAGAGAAGAGATCAGTGCTCAG GTGCTGCTCTCCCTGCGCATTTTGCTACTGATGAAGCCCAGTGTGCTATCCCGAGTCAGCCACCAGGTT GCGTATGGGCTCCATGAACTCCTGAAGACCAATGCAGCCAACATCCACTCAGGTGATGACTGGGCCACA CTCTTCACACTGCTGGAGTGCATCGGCTCAGGTGTGAAGCCTCCAGCTGCTCTGCAGGCCACAGCCAGG GCAGATGCACCTGATGCCGGGGCCCAGTCAGATAGTGAGCTCCCATCCTACCATCAGAATGACGTGAGC CTGGATCGAGGGTACACTTCCGACTCAGAGGTCTACACTGACCATGGCAGGCCGGGCAAGATACACCGA TCAGCCACAGATGCCGATGTGGTCAACAGTGGTTGGTTAGTGGTTGGGAAGGATGACGTTGATAACTCC AAGCCAGGGCCCAGCCGCCCAGGCCCTTCACCCCTGATCAATCAATACAGCCTAACAGTGGGACTGGAT TTGGGGCCACACGACACTAAGTCTCTGCTTAAGTGTGTGGAATCGCTGTCCTTCATCGTGCGTGATGCT GCCCACATCACACCTGACAACTTTGAGCTCTGCGTCAAGACTCTCCGGATCTTTGTGGAGGCCAGTCTG AATGGCGGATGCAAGTCCCAGGAGAAACGTGGCAAGAGTCACAAATATGACAGCAAAGGGAACCGCTTC AAGAAGAAATCCAAAGAGGGATCAATGCTTCGCCGGCCTCGAACCTCCAGCCAACATGCCTCTCGGGGC GGGCAGAGTGATGATGATGAGGACGAAGGCGTGCCTGCCAGCTACCATACGGTGTCTTTACAGTTGCTA GACCTGATGCACACCCTGCACACGCGGGCAGCCTCTATCTACAGCTCATGGGCGGAGGAGCAACGCCAC CTGGAGACAGGTGGCCAGAAGATTGAAGCTGATTCTCGCACCCTCTGGGCCCACTGCTGGTGCCCTTTA CTGCAGGGTATTGCCTGCCTGTGCTGCGATGCCCGGCGCCAGGTACGGATGCAGGCACTGACCTATCTG CAGCGAGCACTACTTGTACATGATCTGCAAAAGCTAGATGCCCTGGAATGGGAGTCCTGTTTTAACAAG GTGCTGTTTCCTCTACTTACCAAGCTCTTGGAGAACATCAGCCCTGCAGATGTGGGTGGGATGGAGGAG ACCCGGATGAGGGCTTCCACATTGCTCTCTAAGGTCTTCCTGCAGCACCTGTCTCCACTGCTGTCACTC TCTACCTTTGCGGCCCTCTGGCTCACCATCTTGGACTTCATGGACAAGTACATGCACGCAGGCTCCAGC GACTTACTGTCAGAGGCGATCCCTGAGTCTCTGAAGAACATGCTTCTGGTGATGGACACAGCGGAGATT TTCCACAGTGCAGATGCACGGGGAGGCGGCCCCTCGGCCCTCTGGGAGATCACCTGGGAACGCATTGAC TGTTTTCTCCCTCACCTACGAGATGAACTCTTCAAGCAGACCGTCATCCAGGACCCCATGCCCATGGAG CCTCAAGGCCAAAAGCCTCTCGCCTCAGCCCACCTGACTTCCGCTGCTGGCGACACTAGGACACCTGGC CATCCACCGCCCCCAGAGATTCCATCTGAGCTGGGGGCCTGTGACTTTGAGAAGCCCGAGAGCCCCCGA GCCGCCAGCAGCAGCTCCCCAGGATCACCAGTGGCCTCAAGCCCCAGCAGGCTGAGCCCCACCCCCGAC GGGCCTCCACCCTTGGCTCAGCCCCCACTGATCCTGCAGCCCTTGGCCTCCCCACTGCAGGTGGGCGTG CCACCTATGACTCTGCCCATCATCCTCAACCCTGCGCTCATCGAGGCCACCTCACCAGTGCCCCTCCTG GCCACACCCCGCCCCACAGATCCCATACCCACCTCTGAGGTCAACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199379
Insert Size	5568 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199379.1
RefSeq Size	6438 bp
RefSeq ORF	5568 bp
Locus ID	8729
UniProt ID	Q92538
MW	206 kDa
Gene Summary	This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

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