GBF1 (NM_001199378) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARF1GEF
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331300 representing NM_001199378. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGGATAAGAATATTTACATCATTCAAGGGGAGATTAACATTGTGGTTGGGGCCATCAAACGAAAT GCCCGATGGAGCACCCATACACCACTGGATGAAGAACGGGATCCTCTGCTGCATAGTTTCGGTCATCTA AAGGAGGTTTTAAACAGTATAACAGAACTCTCAGAAATTGAGCCCAATGTATTCCTTCGACCTTTTCTG GAAGTGATTCGCTCTGAAGATACCACTGGCCCTATCACTGGACTGGCACTCACCTCTGTCAACAAGTTC CTGTCCTATGCACTCATAGATCCCACCCATGAGGGCACAGCAGAGGGCATGGAGAACATGGCAGATGCT GTCACCCATGCTCGTTTTGTGGGCACGGATCCTGCCAGTGATGAAGTTGTCCTGATGAAAATCCTTCAG GTTCTACGGACTCTGCTGCTAACCCCAGTGGGTGCCCACCTAACCAATGAATCTGTGTGTGAGATTATG CAGTCTTGCTTCCGGATCTGCTTTGAAATGAGGCTCAGTGAGTTATTGAGAAAATCCGCAGAGCACACT CTCGTAGACATGGTGCAGCTGCTCTTCACAAGGTTACCTCAGTTTAAAGAAGAACCCAAGAACTATGTG GGGACCAACATGAAGAAGCTGAAAATGAGAGCCGGAGGCATGAGTGATTCATCCAAATGGAAGAAACAG AAGAGATCCCCTCGGCCCCCACGCCATATGACCAAAGTCACACCAGGTTCAGAGCTGCCCACTCCCAAT GGAACCACCTTATCATCTAACCTCACTGGTGGCATGCCCTTCATTGATGTGCCCACTCCCATCTCCTCT GCAAGTTCAGAAGCTGCCTCAGCAGTGGTCAGTCCCTCTACAGACAGTGGCCTGGAATTCTCCTCCCAA ACCACTTCCAAGGAAGACCTTACTGATCTAGAGCAACCTGGCTCTCCAGGGTACAGCACAGCTACAGAG CCTGGAAGCAGTGAGCTAGGTGTTCCCGAGCAGCCTGACCTCCAGCAGGAAGGGACCCATGTGGAAAAG TCCCAGTCAGCATCTGTGGAGTCCATCCCTGAAGTGTTAGAGGAGTGCACGTCCCCTGCCGACCACTCT GACTCTGCCTCTGTCCATGACATGGATTACGTCAATCCCCGGGGCGTGCGCTTTACACAGTCCTCCCAG AAAGAAGGCACAGCTTTGGTCCCCTATGGTCTTCCCTGCATCCGCGAGCTCTTCCGCTTCCTCATCTCC CTCACCAATCCACACGACCGCCATAACTCAGAGGTTATGATTCACATGGGACTGCATTTGCTGACAGTG GCCCTTGAGTCAGCCCCTGTAGCCCAGTGCCAGACCCTCTTGGGCCTCATCAAGGATGAGATGTGCCGT CACTTATTCCAGCTACTCAGCATAGAGCGACTAAACCTTTATGCTGCTTCCCTGCGAGTATGCTTCCTA CTGTTTGAGAGCATGCGAGAGCACCTCAAGTTCCAAATGGAGATGTACATCAAAAAGCTTATGGAGATC ATCACTGTGGAGAACCCCAAGATGCCTTATGAGATGAAGGAGATGGCACTGGAGGCCATTGTGCAGCTC TGGCGCATCCCCAGCTTTGTCACAGAGCTCTACATCAACTATGATTGTGACTACTACTGTTCCAACCTC TTTGAGGAACTCACAAAGCTGCTGTCCAAGAATGCCTTCCCTGTGTCTGGTCAACTCTATACAACACAC CTACTATCTCTTGATGCCCTATTGACAGTGATTGACAGCACCGAGGCCCACTGCCAGGCTAAAGTCCTC AACAGCCTCACCCAGCAAGAGAAGAAGGAGACAGCCAGACCAAGCTGTGAGATAGTAGATGGCACCCGA GAAGCTAGCAATACTGAGAGAACTGCCAGCGATGGGAAAGCTGTAGGCATGGCCTCAGACATCCCAGGC CTGCATCTGCCAGGTGGAGGGCGGCTGCCACCAGAACATGGGAAATCAGGATGCAGTGATCTGGAGGAA GCTGTTGACTCTGGGGCTGACAAAAAGTTTGCCCGGAAGCCACCCCGATTTTCCTGTCTCCTGCCAGAT CCACGGGAACTAATTGAAATTAAAAACAAAAAGAAGCTGCTAATCACTGGCACAGAGCAGTTCAATCAG AAACCAAAGAAGGGGATTCAGTTTCTGCAAGAGAAAGGCCTCCTCACCATCCCAATGGACAACACAGAG GTTGCTCAGTGGCTCCGAGAGAACCCTCGGCTGGACAAGAAGATGATTGGAGAGTTTGTGAGTGACCGC AAAAACATTGACCTGTTGGAGAGCTTTGTGAGCACCTTCAGTTTTCAGGGTCTGCGACTGGACGAAGCC CTCCGCCTCTACCTGGAAGCCTTCCGTTTGCCTGGGGAAGCACCAGTCATCCAGAGGTTGCTGGAGGCA TTCACAGAGCGTTGGATGAATTGTAATGGCTCCCCATTTGCCAATAGCGATGCCTGCTTTTCCCTGGCC TATGCTGTCATCATGCTTAATACTGACCAGCACAACCACAATGTTCGTAAACAGAATGCACCCATGACC CTGGAGGAGTTTCGCAAAAATCTGAAAGGTGTGAATGGAGGCAAGGACTTTGAGCAAGACATCCTGGAG GACATGTACCATGCCATCAAGAATGAGGAAATTGTAATGCCTGAGGAGCAGACAGGCTTGGTTCGGGAG AACTATGTGTGGAATGTGCTGCTTCATCGAGGTGCCACCCCTGAGGGCATATTCCTGCGTGTGCCTACT GCCAGCTATGATCTTGACCTCTTCACCATGACCTGGGGCCCCACTATTGCTGCTCTCTCTTATGTCTTT GACAAAAGCCTTGAGGAGACAATCATCCAGAAAGCCATCTCAGGCTTCAGGAAGTGCGCCATGATCTCC GCCCACTATGGCCTCAGCGATGTGTTTGACAATCTCATCATCTCTCTATGCAAATTCACAGCTCTCAGC AGTGAGTCTATTGAGAACCTGCCCAGTGTATTTGGAAGCAACCCTAAAGCCCATATTGCAGCCAAGACA GTATTCCATTTGGCCCATCGTCATGGTGACATCCTGCGGGAGGGCTGGAAGAATATCATGGAGGCCATG CTGCAGCTCTTCCGAGCCCAACTACTGCCCAAGGCTATGATAGAGGTAGAAGATTTCGTGGATCCCAAT GGCAAGATCTCTCTACAGCGGGAAGAGACACCATCAAACCGAGGAGAGTCAACAGTGCTGAGCTTTGTG AGCTGGCTAACACTGAGTGGTCCTGAGCAGTCTAGTGTTCGGGGCCCATCCACTGAAAACCAAGAGGCC AAGAGAGTGGCCTTAGAGTGTATAAAGCAATGTGACCCAGAAAAAATGATCACAGAAAGCAAGTTCCTC CAGCTGGAGTCACTACAGGAGCTCATGAAGGCTCTGGTCTCAGTGACACCAGATGAAGAGACATATGAT GAGGAAGATGCTGCTTTCTGCCTAGAGATGCTGCTAAGGATTGTGTTGGAGAACAGGGATCGTGTGGGC TGTGTGTGGCAGACTGTTCGAGACCATCTATACCACCTCTGTGTTCAGGCACAAGATTTCTGCTTCCTT GTGGAGCGGGCAGTGGTGGGGTTGCTACGCCTGGCCATTCGGCTTCTCCGGAGAGAAGAGATCAGTGCT CAGGTGCTGCTCTCCCTGCGCATTTTGCTACTGATGAAGCCCAGTGTGCTATCCCGAGTCAGCCACCAG GTTGCGTATGGGCTCCATGAACTCCTGAAGACCAATGCAGCCAACATCCACTCAGGTGATGACTGGGCC ACACTCTTCACACTGCTGGAGTGCATCGGCTCAGGTGTGAAGCCTCCAGCTGCTCTGCAGGCCACAGCC AGGGCAGATGCACCTGATGCCGGGGCCCAGTCAGATAGTGAGCTCCCATCCTACCATCAGAATGACGTG AGCCTGGATCGAGGGTACACTTCCGACTCAGAGGTCTACACTGACCATGGCAGGCCGGGCAAGATACAC CGATCAGCCACAGATGCCGATGTGGTCAACAGTGGTTGGTTAGTGGTTGGGAAGGATGACGTTGATAAC TCCAAGCCAGGGCCCAGCCGCCCAGGCCCTTCACCCCTGATCAATCAATACAGCCTAACAGTGGGACTG GATTTGGGGCCACACGACACTAAGTCTCTGCTTAAGTGTGTGGAATCGCTGTCCTTCATCGTGCGTGAT GCTGCCCACATCACACCTGACAACTTTGAGCTCTGCGTCAAGACTCTCCGGATCTTTGTGGAGGCCAGT CTGAATGGCGGATGCAAGTCCCAGGAGAAACGTGGCAAGAGTCACAAATATGACAGCAAAGGGAACCGC TTCAAGAAGAAATCCAAAGAGGGATCAATGCTTCGCCGGCCTCGAACCTCCAGCCAACATGCCTCTCGG GGCGGGCAGAGTGATGATGATGAGGACGAAGGCGTGCCTGCCAGCTACCATACGGTGTCTTTACAGTTG CTAGACCTGATGCACACCCTGCACACGCGGGCAGCCTCTATCTACAGCTCATGGGCGGAGGAGCAACGC CACCTGGAGACAGGTGGCCAGAAGATTGAAGCTGATTCTCGCACCCTCTGGGCCCACTGCTGGTGCCCT TTACTGCAGGGTATTGCCTGCCTGTGCTGCGATGCCCGGCGCCAGGTACGGATGCAGGCACTGACCTAT CTGCAGCGAGCACTACTTGTACATGATCTGCAAAAGCTAGATGCCCTGGAATGGGAGTCCTGTTTTAAC AAGGTGCTGTTTCCTCTACTTACCAAGCTCTTGGAGAACATCAGCCCTGCAGATGTGGGTGGGATGGAG GAGACCCGGATGAGGGCTTCCACATTGCTCTCTAAGGTCTTCCTGCAGCACCTGTCTCCACTGCTGTCA CTCTCTACCTTTGCGGCCCTCTGGCTCACCATCTTGGACTTCATGGACAAGTACATGCACGCAGGCTCC AGCGACTTACTGTCAGAGGCGATCCCTGAGTCTCTGAAGAACATGCTTCTGGTGATGGACACAGCGGAG ATTTTCCACAGTGCAGATGCACGGGGAGGCGGCCCCTCGGCCCTCTGGGAGATCACCTGGGAACGCATT GACTGTTTTCTCCCTCACCTACGAGATGAACTCTTCAAGCAGACCGTCATCCAGGACCCCATGCCCATG GAGCCTCAAGGCCAAAAGCCTCTCGCCTCAGCCCACCTGACTTCCGCTGCTGGCGACACTAGGACACCT GGCCATCCACCGCCCCCAGAGATTCCATCTGAGCTGGGGGCCTGTGACTTTGAGAAGCCCGAGAGCCCC CGAGCCGCCAGCAGCAGCTCCCCAGGATCACCAGTGGCCTCAAGCCCCAGCAGGCTGAGCCCCACCCCC GACGGGCCTCCACCCTTGGCTCAGCCCCCACTGATCCTGCAGCCCTTGGCCTCCCCACTGCAGGTGGGC GTGCCACCTATGACTCTGCCCATCATCCTCAACCCTGCGCTCATCGAGGCCACCTCACCAGTGCCCCTC CTGGCCACACCCCGCCCCACAGATCCCATACCCACCTCTGAGGTCAACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199378
Insert Size	5571 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199378.1
RefSeq Size	6441 bp
RefSeq ORF	5571 bp
Locus ID	8729
UniProt ID	Q92538
MW	206.1 kDa
Gene Summary	This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (2) uses multiple alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.