MAG (NM_001199216) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GMA; S-MAG; SIGLEC-4A; SIGLEC4A; SPG75
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331268 representing NM_001199216. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCTCGTCCATCTCGGCCTTCGAAGGCACGTGCGTCTCCATCCCCTGCCGCTTTGACTTCCCGGAT GAGCTGCGGCCCGCTGTGGTGCATGGTGTCTGGTACTTCAATAGCCCCTACCCCAAGAACTACCCCCCG GTGGTCTTCAAGTCGCGCACCCAAGTAGTCCACGAGAGCTTCCAGGGCCGCAGCCGCCTCCTGGGGGAC CTGGGCCTGCGAAACTGCACCCTCCTGCTCAGCAACGTCAGCCCCGAGCTGGGCGGGAAGTACTACTTC CGTGGGGACCTGGGCGGCTACAACCAGTACACCTTCTCAGAGCACAGCGTCCTGGATATCGTCAACACC CCCAACATCGTGGTGCCCCCAGAGGTGGTGGCAGGCACGGAGGTGGAGGTCAGCTGCATGGTGCCGGAC AACTGCCCAGAGCTGCGCCCTGAGCTGAGCTGGCTGGGCCACGAGGGGCTGGGGGAGCCCGCTGTGCTG GGCCGGCTGCGGGAGGACGAGGGCACCTGGGTGCAGGTGTCACTGCTGCACTTCGTGCCCACGAGGGAG GCCAACGGCCACAGGCTGGGCTGCCAGGCCTCCTTCCCCAACACCACCCTGCAGTTCGAGGGCTACGCC AGCATGGACGTCAAGTACCCCCCGGTGATTGTGGAGATGAACTCCTCGGTGGAGGCCATCGAGGGCTCC CACGTGAGCCTGCTCTGTGGGGCTGACAGCAACCCCCCGCCGCTGCTGACCTGGATGCGGGACGGGACA GTCCTCCGGGAGGCGGTGGCCGAGAGCCTGCTCCTGGAGCTGGAGGAGGTGACCCCCGCCGAAGACGGC GTCTATGCCTGCCTGGCCGAGAATGCCTATGGCCAGGACAACCGCACCGTGGGGCTCAGTGTCATGTAT GCACCCTGGAAGCCAACAGTGAACGGGACAATGGTGGCCGTAGAGGGGGAGACGGTCTCTATCTTGTGC TCCACACAGAGCAACCCGGACCCTATTCTCACCATCTTCAAGGAGAAGCAGATCCTGTCCACGGTCATC TACGAGAGCGAGCTGCAGCTGGAGCTGCCGGCCGTGTCACCCGAGGATGATGGAGAGTACTGGTGTGTG GCTGAGAACCAGTATGGCCAGAGGGCCACCGCCTTCAACCTGTCTGTGGAGTTCGCCCCTGTGCTCCTC CTGGAGTCCCACTGCGCGGCAGCCCGAGACACGGTGCAGTGCCTGTGCGTGGTGAAGTCCAACCCGGAG CCGTCCGTGGCCTTTGAGCTGCCATCGCGCAATGTGACCGTGAACGAGAGCGAGCGGGAGTTCGTGTAC TCGGAGCGCAGCGGCCTCGTGCTCACCAGCATCCTCACGCTGCGGGGGCAGGCCCAGGCCCCGCCCCGC GTCATCTGCACCGCGAGGAACCTCTATGGCGCCAAGAGCCTGGAGCTGCCCTTCCAGGGAGCCCATCGA CTGATGTGGGCCAAGATCGGGCCTGTGGGCGCCGTGGTCGCCTTTGCCATCCTGATTGCCATCGTCTGC TACATTACCCAGACACGCAGGAAAAAGAACGTGACAGAGAGCCCCAGCTTCTCGGCAGGGGACAACCCT CCCGTCCTGTTCAGCAGCGACTTCCGCATCTCTGGGGCACCAGAGAAGTACGAGAGCGAGAGGCGCCTG GGATCTGAGAGGAGGCTGCTGGGCCTTCGGGGTGAGCCCCCAGAGCTGGACCTGAGCTATTCTCACTCG GACCTGGGGAAACGGCCCACCAAGGACAGCTACACGCTGACGGAGGAGCTAGCTGAGTATGCTGAAATC CGGGTCAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199216
Insert Size	1806 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199216.1
RefSeq Size	2445 bp
RefSeq ORF	1806 bp
Locus ID	4099
UniProt ID	P20916
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)
MW	66.2 kDa
Gene Summary	The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a.

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