BRCC45 (BRE) (NM_001261840) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRCC4; BRCC45; BRE
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330733 representing NM_001261840. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCCCCAGAAGTGGCCTTGAACCGAATATCTCCAATGCTCTCCCCTTTCATATCTAGCGTGGTCCGG AATGGAAAAGTGGGACTGGATGCTACAAACTGTTTGAGGATAACTGACTTAAAATCTGGCTGCACATCA TTGACTCCTGGGCCCAACTGTGACCGATTTAAACTGCACATACCATATGCTGGAGAGACATTAAAGTGG GATATCATTTTCAATGCCCAATACCCAGAACTGCCTCCCGATTTTATCTTTGGAGAAGATGCTGAATTC CTGCCAGACCCCTCAGCTTTGCAGAATCTTGCCTCCTGGAATCCTTCAAATCCTGAATGTCTCTTACTT GTGGTGAAGGAACTTGTGCAACAATATCACCAATTCCAATGTAGCCGCCTCCGGGAGAGCTCCCGCCTC ATGTTTGAATACCAGACATTACTGGAGGAGCCACAGTATGGAGAGAACATGGAAATTTATGCTGGGAAA AAAAACAACTGGACTGGTGAATTTTCAGCTCGTTTCCTTTTGAAGCTGCCCGTAGATTTCAGCAATATC CCCACATACCTTCTCAAGGATGTAAATGAAGACCCTGGAGAAGATGTGGCCCTCCTCTCTGTTAGTTTT GAGGACACTGAAGCCACCCAGGTGTACCCCAAGCTGTACTTGTCACCTCGAATTGAGCATGCACTTGGA GGCTCCTCAGCTCTTCATATCCCAGCTTTTCCAGGAGGAGGATGTCTCATTGATTACGTTCCTCAAGTA TGCCACCTGCTCACCAACAAGGTGCAGTACGTGATTCAAGGGTATCACAAAAGAAGAGAGTATATTGCT GCTTTTCTCAGTCACTTTGGCACAGGTGTCGTGGAATATGATGCAGAAGGCTTTACAAAACTCACTCTG CTGCTGATGTGGAAAGATTTTTGTTTTCTTGTACACATTGACCTGCCTCTGTTTTTCCCTCGAGACCAG CCAACTCTCACATTTCAGTCCGTTTATCACTTTACCAACAGTGGACAGCTTTACTCCCAGGCCCAAAAA AATTATCCGTACAGCCCCAGATGGGATGGAAATGAAATGGCCAAAAGAGCAAATTCCAGGAGGCAGCAT TTGCCAATGGAAAGCTCTAGGAAACACCAGTCTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001261840
Insert Size	1140 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001261840.1
RefSeq Size	1776 bp
RefSeq ORF	1140 bp
Locus ID	9577
UniProt ID	Q9NXR7
Protein Families	Druggable Genome
MW	43.2 kDa
Gene Summary	This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer. [provided by RefSeq, Jun 2016]

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