SLC4A8 (NM_001258402) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NBC3; NDCBE
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330679 representing NM_001258402. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCGGCCGCCGGGAGTAACGAGCCGGACGGCGTCCTCAGCTATCAGAGACCAGATGAAGAAGCTGTG GTGGATCAGGGTGGGACCAGTACAATTCTCAACATTCACTATGAAAAAGAAGAGCTGGAAGGTCACAGA ACTCTGTATGTGGGAGTTCGGATGCCGCTTGGCCGGCAGAGCCATCGGCATCACCGCACTCATGGCCAG AAGCACCGGAGACGAGGGCGGGGCAAAGGAGCCAGCCAGGGGGAGGAAGGCCTGGAAGCCCTGGCCCAC GACACACCATCTCAGCGTGTTCAGTTCATTCTTGGCACCGAGGAAGATGAAGAGCATGTGCCTCATGAG CTGTTTACAGAGCTGGATGAGATCTGTATGAAAGAGGGAGAAGATGCTGAGTGGAAGGAAACAGCCAGG TGGCTGAAGTTTGAAGAAGATGTTGAAGATGGGGGAGAACGCTGGAGCAAGCCTTATGTGGCAACCCTT TCATTGCACAGCCTGTTTGAGCTAAGGAGCTGCCTTATTAATGGAACAGTCCTCCTGGATATGCATGCA AATAGCATAGAAGAAATTTCAGACCTGATCCTGGATCAGCAAGAACTGTCCAGTGACCTGAATGACAGC ATGAGGGTTAAAGTGCGGGAAGCCCTTCTCAAAAAGCATCATCATCAGAATGAAAAGAAGAGAAACAAC CTCATTCCCATTGTTCGCTCCTTTGCTGAGGTTGGCAAGAAGCAGTCTGATCCTCATTTGATGGATAAA CATGGTCAAACCGTGTCTCCTCAGTCTGTTCCAACTACAAATCTTGAAGTAAAAAATGGAGTGAATTGT GAACATAGTCCTGTGGATTTAAGCAAGGTAGACCTTCATTTCATGAAAAAAATTCCTACTGGGGCCGAG GCCTCCAATGTCCTGGTTGGAGAGGTGGATATTTTGGACCGTCCCATTGTTGCCTTTGTGAGGCTGTCT CCAGCTGTTCTTCTCTCAGGCCTAACAGAAGTGCCAATCCCAACAAGATTTTTGTTTATCTTATTGGGT CCAGTAGGGAAAGGTCAGCAGTACCATGAGATTGGCAGATCCATGGCCACCATCATGACAGATGAGATT TTTCATGACGTAGCATATAAGGCAAAAGAGCGAGATGATCTCCTGGCGGGGATTGATGAGTTCCTAGAC CAGGTGACGGTGCTCCCTCCAGGAGAGTGGGATCCCTCCATTAGAATTGAGCCACCCAAAAATGTCCCT TCCCAGGAGAAAAGGAAAATGCCTGGAGTTCCAAATGGAAATGTTTGCCACATAGAACAGGAACCACAT GGGGGTCACAGTGGGCCAGAACTTCAGCGCACTGGGCGGCTATTTGGGGGCTTGGTGCTGGACATCAAG CGGAAGGCCCCCTGGTACTGGAGCGACTACCGAGATGCACTCAGCTTACAGTGTTTGGCTTCCTTTCTG TTCCTGTACTGTGCCTGCATGTCACCTGTCATCACCTTTGGGGGACTGCTTGGAGAAGCCACTGAGGGA CGCATAAGTGCAATTGAATCCTTGTTTGGAGCTTCCATGACTGGGATTGCTTATTCCTTGTTTGCGGGA CAGGCTCTCACCATCCTGGGAAGTACTGGACCAGTGCTTGTGTTTGAAAAGATTTTGTTCAAATTCTGC AAAGACTATGCTCTTTCATACCTCTCCCTGCGAGCTTGTATTGGACTGTGGACCGCTTTCCTGTGTATT GTCCTTGTGGCAACTGATGCCAGTTCCCTTGTCTGCTACATTACCCGTTTCACTGAAGAAGCATTTGCC TCCCTAATTTGCATTATTTTCATCTATGAAGCAATAGAAAAACTGATTCACCTGGCAGAGACCTACCCC ATCCACATGCACAGCCAGCTGGACCACCTTAGCCTCTATTACTGCAGGTGTACTCTGCCAGAGAATCCA AACAATCACACCCTCCAGTACTGGAAGGACCACAACATCGTGACAGCAGAAGTCCACTGGGCTAACCTG ACTGTCAGTGTAAGTCTGGGAGCTGCCAGATGTCCTAGCATTGTGACCACAGGTTTAGGAGGGACTTCT AAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001258402
Insert Size	2076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258402.1
RefSeq Size	2332 bp
RefSeq ORF	2076 bp
Locus ID	9498
UniProt ID	Q2Y0W8
Protein Families	Druggable Genome, Transmembrane
MW	77.1 kDa
Gene Summary	The protein encoded by this gene is a membrane protein that functions to transport sodium and bicarbonate ions across the cell membrane. The encoded protein is important for pH regulation in neurons. The activity of this protein can be inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (4) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (d) has a shorter and distinct C-terminus compared to isoform a.

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