ZNF451 (NM_001257273) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	COASTER; dJ417I1.1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330591 representing NM_001257273. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGAGACCCGGGGTCGGAGATAATAGAATCTGTCCCTCCAGCTGGCCCTGAGGCATCTGAGTCAACA ACGGATGAAAATGAAGACGACATTCAGTTTGTCAGTGAAGGACCATTACGACCTGTTCTTGAATACATT GATCTGGTCAGCAGTGATGATGAAGAGCCTAGCACCTCTTATACTGATAGAATGCCTGAATCTAAGGTG CCATCCTCTGAGAATCATCGCCCAGAAATGTGCTCTAGCTGCAATGTTCCTCTTCCCATTGGAGATAGC AGCTCCTTCTCTGGGAGTTGTTCCAGCAGTCCAGAAAGGATAGTTTCTCAAACTTCCTCTGTTGAGAAC CCATTGGAGAACCAGAAAAATGATCAAAATAATTCAGATACTAAGATCTCTGAGACAGAGACCCTTAAA TCATCACAGAATTTTCAGACTCTGCCTTCATCTCCACTTCTGGTCCCCCAAGAATCTTTGGCCTCTTCT GAGGTCAAAGAGAATTTACGTATAGATTCTTCTTCAGCTTCACAGCATGGACGGGATGCCATCCTCTAT CTCCAGACACAAGTAGCTGAAATGTCCCGAGTGATACGTGATCTGCAGTCCAGGAGCTGTTTTAGATTT CATCATTCTAGGCCAAGTGAGAACTCCTCAGTTCCTTGGGACATCTCCACCTCTAAAGAGGAAAATTTA TCCACAGTTGAAGAAGAAACTGATTACAAATCACCATCAGCTGATGACAAAGGGCAGCCATCTGACCCC AGCCAATCTAGTTTCACAGGTCTTTTGAAGAGAATGGAACAAAGAGGTGTTATAAAAAGGGTGACATTA CAATCTGAAGCGGAGTCATGTGAAGGGAAACCTGATTGTGTGACTTCTAAAAAACGTTTGGTTCCTCCA TTGCATCCTCTTCTGAGAATTGCCACCACTGAGGTTTTTAAAGACCCTGCTGATTGCCATCCTTCTTCC TTCATGGGACACAGGGTATATCCTGTGGCCAAGGACACCTCTCCTTTCCAACCAAACCCACCAGCTGAA GGCCCCATTGTAGAAGCATTAGAACACAGCAAAAGAGGAAACACAACATCCCCTCTAGATTCTACCTCA AAAGAAATGGAGGTCATGGGTTGTAGGTTTTACCACGCTGCCTCCATTGCAGCCCGAGCTGCTAGCTAC ATGGCCTATATGACTCAATATCAGCGTAAACTCTGGGAAGACATGGAAGATCTGGTTCATGACCCAGAA TTTGATCGTGGAAAAGCAAGATGCATAATATCTGATGGTATGGATGCAGGCCTTTGGCAACTTTGTACT ACTAGGGACATAATGGACTCTGTAGTCAGAGTTATGGCCATGGCCATAGACTATAGACGGCAGGCCTGG CTTCGACTTACATCTCTCACTAAGAAAACCCAGGAGAAGATCTCCCACTTGCCCTTTGATGGTACTTCC CTTTTTGGACAAGATGTGAAAGCTGTTGTTGCAGAAGACAACAATATAAAAGAAAATGACTATAAAGAT CACAAATACTATAATCAGCATCGATACTTTTATAGTCATGATCAGAAAGCACATTATCACAATAGAGGA TACTCCAAAGGGGATTGGTACAAACCTCGAAACCACCCCTATAGATATAGAAAGAAGGGAGACTCTCCA GAACGCCATGGGTACAAGAATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257273
Insert Size	1680 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257273.1
RefSeq Size	9558 bp
RefSeq ORF	1680 bp
Locus ID	26036
Protein Families	Transcription Factors
MW	63 kDa
Gene Summary	E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins (PubMed:26524493, PubMed:26524494). Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation (By similarity). Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11' (PubMed:26524493). Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9' (PubMed:24324267). Plays a role in regulating the transcription of AR targets (PubMed:18656483).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1.

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