MCM BP (MCMBP) (NM_001256379) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C10orf119; MCM-BP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330406 representing NM_001256379. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACCTACAGCCCAATAAGCAGAAAGACCAACATGCAGGTGCCAGACAAGCAGGGAGTGTTGGTGGT CTTCAATGGTGTGGAGAGCCAAAACGTTTAGAAACTGAAGCTTCTACTGGGCAACAGCTGAACTCTCTG AACTTGTCTTCTCCTTTTGATTTGAATTTTCCATTGCCAGGAGAGAAGGGCCCTGCATGCCTTGTGAAG GTTTATGAAGATTGGGATTGTTTCAAAGTAAATGACATTCTTGAGCTATATGGCATACTGTCTGTGGAT CCTGTGCTGAGTATACTGAATAATGATGAAAGGGATGCCTCTGCACTGCTGGATCCGATGGAGTGCACA GACACAGCAGAGGAGCAGAGAGTACACAGTCCTCCTGCTTCATTAGTGCCGAGAATTCATGTGATCTTA GCCCAGAAGTTGCAACACATCAACCCATTATTGCCTGCCTGCCTTAACAAAGAGGAGAGCAAAACCTTT GTTTCAAGTTTCATGTCCGAATTGTCTCCAGTCAGAGCAGAACTTCTTGGGTTCCTTACTCATGCCCTT CTGGGGGATAGTTTGGCTGCTGAATACCTTATATTACATCTCATCTCCACAGTATATACAAGAAGAGAT GTCCTTCCACTAGGAAAATTTACAGTTAACTTGAGTGGTTGCCCACGGAATAGTACCTTCACAGAACAC TTGTATCGAATTATTCAACATCTTGTTCCAGCATCTTTTCGTCTGCAGATGACTATAGAGAACATGAAC CATTTGAAATTCATTCCCCACAAAGACTACACAGCCAATCGCTTGGTCAGTGGGCTCCTCCAGCTGCCC AGCAATACTTCCCTTGTAATCGATGAGACTCTCCTGGAACAGGGGCAGCTGGATACCCCAGGTGTTCAT AATGTGACAGCCCTGAGCAACCTCATAACGTGGCAGAAGGTGGATTATGACTTCAGCTACCATCAGATG GAATTCCCCTGCAATATTAACGTTTTCATTACTTCGGAGGGGAGGTCACTCCTCCCGGCAGACTGCCAG ATTCACTTACAGCCCCAGCTAATTCCACCAAACATGGAGGAGTACATGAACAGCCTTCTCTCAGCGGTG CTGCCTTCCGTGCTGAACAAATTCCGCATTTATCTAACTCTTTTGAGATTCTTGGAATATAGCATATCT GATGAAATAACCAAGGCAGTTGAAGATGACTTTGTGGAAATGCGGAAGAACGACCCTCAGAGCATCACT GCTGATGATCTTCACCAGCTGCTCGTGGTGGCTCGGTGTCTGTCTCTCAGTGCTGGTCAGACAACGCTG TCAAGAGAACGATGGCTGAGAGCAAAGCAGCTAGAGTCTTTAAGAAGAACGAGGCTTCAGCAGCAAAAA TGTGTGAATGGAAATGAACTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256379
Insert Size	1404 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256379.1
RefSeq Size	4102 bp
RefSeq ORF	1404 bp
Locus ID	79892
UniProt ID	Q9BTE3
MW	52.7 kDa
Gene Summary	This gene encodes a protein which is a component of the hexameric minichromosome maintenance (MCM) complex which regulates initiation and elongation of DNA. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (3) differs in the 5' UTR and coding region, and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter and has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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