Gemin 5 (GEMIN5) (NM_001252156) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GEMIN-5; NEDCAM
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330276 representing NM_001252156. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGCAGGAGCCGCGGACGCTGCCGCCCTCCCCCAACTGGTACTGCGCCCGCTGCAGCGATGCCGTG CCCGGGGGCCTCTTTGGCTTCGCCGCGCGGACCTCCGTCTTCCTTGTCCGCGTGGGCCCGGGCGCAGGC GAGAGTCCAGGGACACCCCCGTTTCGAGTCATAGGAGAGTTGGTGGGACACACCGAAAGGGTCTCTGGC TTCACATTTTCTCATCACCCTGGTCAGTACAACCTCTGTGCCACCAGCTCCGACGATGGGACTGTGAAA ATATGGGATGTAGAGACAAAAACAGTTGTGACAGAACATGCACTCCATCAGCATACGATATCAACATTA CATTGGTCTCCTCGAGTAAAGGACTTAATAGTATCTGGGGATGAAAAAGGAGTAGTTTTCTGTTACTGG TTTAACAGAAATGACAGCCAGCACCTCTTTATAGAACCCAGGACAATTTTCTGTCTTACTTGTTCACCT CATCATGAAGATTTAGTAGCCATTGGCTACAAGGATGGCATAGTGGTGATAATTGACATCAGTAAGAAA GGAGAAGTTATTCATAGGCTTCGAGGCCATGATGATGAAATCCACTCCATAGCCTGGTGTCCCCTGCCT GGTGAAGATTGTTTATCTATAAACCAAGAGGAAACTTCAGAAGCTGAAATTACCAACGGGAATGCTGTA GCACAAGCTCCAGTAACAAAAGGTTGCTACTTAGCCACTGGAAGCAAAGATCAAACCATTCGAATCTGG AGCTGTTCTAGAGGCCGAGGGGTGATGATTTTGAAATTGCCCTTTCTGAAGAGAAGAGGAGGGGGTATA GACCCAACTGTTAAAGAGCGCCTTTGGTTGACACTCCATTGGCCCAGCAATCAACCAACACAGCTGGTA TCTAGCTGTTTTGGAGGTGAACTGTTGCAATGGGATCTCACTCAATCTTGGAGACGGAAATACACCCTC TTCAGTGCCTCATCAGAAGGGCAAAATCATTCAAGAATTGTGTTTAATTTATGTCCTTTACAAACAGAG GATGACAAACAGCTATTACTTTCTACATCAATGGATAGAGATGTAAAATGTTGGGACATAGCCACCTTG GAGTGCAGCTGGACCCTTCCTTCCCTTGGTGGGTTTGCATACAGCCTGGCTTTCTCTTCTGTGGACATA GGCTCTTTGGCCATAGGTGTTGGGGATGGCATGATCCGTGTATGGAATACACTCTCCATAAAGAACAAC TATGATGTGAAAAATTTTTGGCAAGGCGTGAAGTCCAAGGTTACAGCGCTGTGCTGGCACCCAACCAAG GAAGGTTGCTTAGCTTTTGGAACTGATGATGGAAAAGTGGGATTGTATGACACCTACTCCAACAAGCCT CCACAGATTTCTAGCACATATCATAAGAAGACTGTATATACTTTAGCCTGGGGGCCACCAGTACCCCCC ATGTCACTTGGAGGAGAAGGAGACAGACCTTCCCTTGCTTTATACAGCTGTGGAGGAGAAGGGATTGTC TTACAGCATAATCCCTGGAAGCTTAGTGGAGAAGCCTTTGACATCAACAAACTCATCAGGGACACCAAT TCAATCAAATACAAATTGCCTGTACACACAGAGATAAGTTGGAAAGCAGATGGCAAAATCATGGCTCTT GGCAATGAAGATGGATCAATAGAAATATTTCAGATTCCCAACCTGAAACTGATCTGTACTATCCAACAG CATCACAAGCTTGTGAATACCATTAGCTGGCATCATGAGCATGGCAGCCAGCCAGAATTGAGCTATCTG ATGGCCTCTGGCTCCAACAATGCAGTCATTTACGTGCACAACCTGAAGACTGTCATAGAGAGCAGCCCT GAGTCTCCAGTGACCATTACAGAGCCCTACCGGACCCTCTCAGGGCATACGGCCAAGATTACCAGTGTG GCGTGGAGCCCACATCATGATGGAAGGCTGGTATCTGCTTCCTATGATGGTACAGCCCAGGTGTGGGAT GCTCTCCGGGAAGAGCCCCTGTGCAATTTCCGAGGACATCGAGGTCGACTGCTTTGTGTGGCATGGTCT CCTTTGGATCCAGACTGCATCTATTCAGGGGCAGATGACTTTTGTGTGCACAAGTGGCTCACTTCCATG CAAGATCATTCCCGGCCTCCTCAAGGCAAAAAAAGTATTGAATTGGAGAAAAAACGGCTCTCTCAACCT AAGGCAAAGCCCAAAAAGAAGAAAAAGCCCACCTTGAGAACTCCTGTAAAGCTGGAATCGATTGATGGA AATGAAGAAGAAAGCATGAAGGAGAACTCAGGACCTGTTGAGAATGGTGTGTCAGACCAAGAAGGGGAG GAGCAAGCACGGGAGCCGGAATTACCCTGTGGCCTTGCTCCAGCGGTTTCTAGAGAACCAGTTATCTGC ACTCCAGTTTCCTCAGGCTTTGAAAAGTCAAAAGTCACCATTAATAACAAAGTCATTTTACTGAAAAAG GAGCCACCAAAAGAGAAGCCAGAAACCTTAATCAAGAAGAGAAAAGCTCGTTCCTTGCTTCCCCTGAGT ACAAGCCTGGACCACAGATCCAAAGAGGAGCTTCATCAGGACTGTTTGGTACTAGCAACTGCAAAGCAC TCCAGAGAGCTGAATGAAGATGTGTCTGCTGATGTTGAGGAAAGATTTCATCTGGGGCTTTTCACAGAC AGGGCTACCCTGTATAGAATGATTGATATTGAAGGAAAAGGTCACTTAGAAAATGGCCACCCTGAGTTA TTTCACCAGCTTATGCTTTGGAAAGGAGATCTCAAAGGTGTTCTCCAGACTGCAGCAGAAAGAGGGGAG CTGACAGACAACCTTGTGGCTATGGCACCAGCAGCTGGCTACCATGTGTGGCTATGGGCTGTGGAAGCT TTTGCCAAACAGCTGTGTTTTCAGGATCAGTATGTCAAGGCTGCTTCTCACCTACTTTCCATCCACAAA GTGTATGAAGCGGTGGAGCTGCTCAAGTCAAACCATTTTTACAGGGAAGCTATTGCGATTGCCAAGGCC CGGCTGCGCCCGGAGGACCCAGTCCTGAAGGACTTGTACCTCAGCTGGGGAACCGTCCTAGAAAGAGAT GGCCACTATGCTGTAGCTGCCAAATGCTATTTAGGGGCCACTTGTGCTTATGATGCAGCCAAAGTTTTG GCCAAAAAGGGGGATGCGGCATCACTTAGAACGGCTGCAGAGTTGGCTGCCATCGTAGGAGAGGATGAG TTGTCTGCTTCCCTGGCTCTCAGATGTGCCCAAGAGCTGCTTCTGGCCAACAACTGGGTGGGAGCCCAG GAAGCCCTGCAGCTGCATGAAAGTCTACAGGGTCAGAGATTGGTGTTTTGCCTTCTGGAGCTACTGTCC AGGCATCTGGAGGAAAAGCAGCTTTCAGAGGGCAAAAGCTCCTCCTCTTACCACACTTGGAACACGGGC ACCGAAGGGCCTTTCGTGGAGAGGGTGACTGCAGTGTGGAAGAGCATCTTCAGCCTTGACACCCCTGAG CAGTATCAGGAAGCCTTTCAGAAGCTGCAGAACATCAAGTACCCATCTGCTACAAATAACACACCTGCC AAACAGCTCCTGCTTCACATTTGCCATGACTTGACCCTGGCAGTGCTGAGCCAACAGATGGCCTCCTGG GACGAGGCTGTGCAGGCGCTCCTTCGGGCGGTGGTCCGGAGCTATGACTCAGGGAGCTTCACCATCATG CAGGAAGTGTACTCAGCCTTTCTCCCTGACGGCTGTGACCACCTAAGAGACAAGTTGGGGGACCATCAA TCCCCTGCCACACCAGCTTTCAAAAGTTTGGAGGCCTTTTTTCTTTATGGGCGTCTGTATGAATTCTGG TGGTCTCTCTCCAGACCTTGCCCAAATTCCAGTGTCTGGGTAAGGGCTGGTCACAGAACACTCTCTGTT GAGCCAAGCCAGCAGTTAGACACTGCCAGCACTGAAGAAACGGACCCTGAAACTTCTCAGCCAGAGCCA AACAGGCCTTCAGAACTAGACTTGAGACTCACAGAAGAAGGTGAGCGAATGCTGAGTACTTTTAAGGAG CTCTTTTCAGAAAAGCATGCCAGTCTCCAAAACTCACAGAGAACTGTTGCTGAAGTCCAAGAGACCTTG GCAGAAATGATCCGACAACACCAAAAGAGTCAACTCTGTAAATCCACAGCAAATGGTCCTGATAAGAAT GAACCGGAAGTAGAAGCAGAGCAGCCCCTCTGCAGTTCTCAGAGCCAGTGTAAAGAAGAAAAAAATGAG CCACTTTCTCTGCCTGAGTTAACCAAAAGGCTTACCGAGGCAAATCAGAGAATGGCGAAATTTCCTGAG AGCATTAAGGCCTGGCCCTTCCCAGATGTGCTGGAGTGCTGCCTCGTCCTGCTTCTCATCAGGTCCCAC TTTCCTGGCTGTCTGGCCCAGGAAATGCAGCAGCAGGCCCAAGAGCTCCTTCAGAAATACGGCAACACG AAAACTTACAGAAGACACTGCCAGACCTTCTGTATGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001252156
Insert Size	4524 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252156.1
RefSeq Size	5401 bp
RefSeq ORF	4524 bp
Locus ID	25929
UniProt ID	Q8TEQ6
Protein Families	Druggable Genome
MW	168.5 kDa
Gene Summary	This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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