ZFP161 (ZBTB14) (NM_001243702) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZF5; ZFP-5; ZFP-161; ZFP161; ZNF478
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330161 representing NM_001243702. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGTTTTTCATCAGTATGTCTGAAACCATTAAATATAATGACGATGATCATAAAACTCTGTTTCTG AAAACACTAAATGAACAACGCCTGGAAGGAGAATTTTGTGATATTGCTATTGTGGTTGAGGATGTGAAA TTCAGAGCACACAGATGTGTTCTTGCTGCCTGCAGCACTTACTTTAAAAAGCTTTTCAAGAAGCTTGAG GTTGATAGTTCTTCGGTCATAGAAATAGATTTTCTTCGTTCTGATATATTTGAAGAGGTCCTGAACTAC ATGTACACAGCAAAGATTTCCGTGAAAAAAGAAGATGTTAACTTAATGATGTCATCGGGTCAGATTCTT GGTATCCGATTTTTGGATAAACTGTGTTCTCAGAAGCGTGATGTGTCCAGTCCCGATGAAAACAATGGT CAGTCCAAAAGTAAGTATTGCCTTAAAATAAATCGCCCCATTGGAGATGCTGCTGACACCCAGGATGAT GATGTAGAGGAAATCGGGGATCAGGATGACAGTCCTTCTGATGACACAGTAGAAGGCACACCCCCGAGT CAGGAGGACGGCAAGTCGCCCACCACAACGCTCAGGGTTCAGGAAGCGATCCTGAAAGAGCTGGGGAGT GAGGAAGTTCGGAAGGTCAATTGCTACGGCCAGGAAGTAGAATCCATGGAGACCCCAGAATCAAAAGAC TTGGGGTCCCAGACCCCTCAAGCCTTAACATTTAATGATGGGATGAGTGAAGTGAAAGATGAACAGACA CCAGGCTGGACAACAGCCGCCAGTGACATGAAGTTTGAGTATTTGCTTTATGGTCACCATCGGGAGCAG ATTGCCTGCCAGGCGTGTGGGAAGACGTTTTCTGATGAAGGCAGATTGAGGAAGCATGAGAAACTCCAC ACGGCGGACAGGCCATTTGTTTGTGAAATGTGCACAAAAGGTTTCACCACACAGGCCCACCTGAAAGAA CACCTAAAAATCCACACAGGATATAAGCCCTATAGCTGTGAGGTGTGTGGAAAATCATTTATCCGTGCC CCAGACTTAAAGAAGCATGAGAGAGTTCACAGTAATGAAAGACCGTTTGCGTGCCACATGTGTGACAAA GCCTTCAAACACAAGTCTCACCTCAAGGATCATGAAAGAAGACACAGAGGGGAAAAGCCTTTTGTGTGT GGCTCCTGCACCAAGGCATTTGCCAAGGCATCTGATCTGAAAAGGCACGAGAACAATATGCACAGTGAA AGGAAGCAGGTTACCCCCAGTGCCATCCAGAGCGAGACAGAACAGTTGCAGGCGGCAGCGATGGCTGCG GAAGCAGAACAGCAGCTGGAGACGATAGCCTGTAGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001243702
Insert Size	1350 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243702.1
RefSeq Size	3363 bp
RefSeq ORF	1350 bp
Locus ID	7541
UniProt ID	O43829
Protein Families	Transcription Factors
MW	51 kDa
Gene Summary	Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene. Transcriptional repressor of MYC and thymidine kinase promoters.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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