CNTN4 (NM_001206956) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AXCAM; BIG-2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329865 representing NM_001206956. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAGAAAATGTCTTTTGGGAATGTAAAGCAAATGGAAGGCCTAAGCCTACATACAAGTGGCTAAAA AATGGCGAACCTCTGCTAACTCGGGATAGAATTCAAATTGAGCAAGGAACACTCAACATAACAATAGTG AACCTCTCAGATGCTGGCATGTATCAGTGTTTGGCAGAGAATAAACATGGAGTTATCTTTTCCAACGCA GAGCTTAGTGTTATAGCTGTAGGTCCAGATTTTTCAAGAACACTCTTGAAAAGAGTAACTCTTGTCAAA GTGGGAGGTGAAGTTGTCATTGAGTGTAAGCCAAAAGCGTCTCCAAAACCTGTTTACACCTGGAAGAAA GGAAGGGATATATTAAAAGAAAATGAAAGAATTACCATTTCTGAAGATGGAAACCTCAGAATCATCAAC GTTACTAAATCAGACGCTGGGAGTTATACCTGTATAGCCACTAACCATTTTGGAACTGCTAGCAGTACT GGAAACTTGGTAGTGAAAGATCCAACAAGGGTAATGGTACCCCCTTCCAGTATGGATGTCACTGTTGGA GAGAGTATTGTTTTACCGTGCCAGGTAACGCATGATCACTCGCTAGACATCGTGTTTACTTGGTCATTT AATGGACACCTGATAGACTTTGACAGAGATGGGGACCACTTTGAAAGAGTTGGAGGGGATTCAGCTGGT GATTTGATGATCCGAAACATCCAACTGAAGCATGCTGGGAAATATGTCTGCATGGTCCAAACAAGTGTG GACAGGCTATCTGCTGCTGCAGACCTGATTGTAAGAGGTCCTCCAGGTCCCCCAGAGGCTGTGACAATA GACGAAATCACAGATACCACTGCTCAGCTCTCCTGGAGACCCGGGCCTGACAACCACAGCCCCATCACC ATGTATGTCATTCAAGCCAGGACTCCATTCTCCGTGGGCTGGCAAGCAGTCAGTACAGTCCCAGAACTC ATTGATGGGAAGACATTCACAGCGACCGTGGTGGGTTTGAACCCTTGGGTTGAATATGAATTCCGCACA GTTGCAGCCAACGTGATTGGGATTGGGGAGCCCAGCCGCCCCTCAGAGAAACGGAGAACAGAAGAAGCT CTCCCCGAAGTCACACCAGCGAATGTCAGTGGTGGCGGAGGCAGCAAATCTGAACTGGTTATAACCTGG GAGACGGTCCCTGAGGAATTACAGAATGGTCGAGGCTTTGGTTATGTGGTGGCCTTCCGGCCCTACGGT AAAATGATCTGGATGCTGACAGTGCTGGCCTCAGCTGATGCCTCTAGATACGTGTTCAGGAATGAGAGC GTGCACCCCTTCTCTCCCTTTGAGGTTAAAGTAGGTGTCTTCAACAACAAAGGAGAAGGCCCTTTCAGT CCCACCACGGTGGTGTATTCTGCAGAAGAAGAACCCACCAAACCACCAGCCAGTATCTTTGCCAGAAGT CTTTCTGCCACAGATATTGAAGTTTTCTGGGCCTCCCCACTGGAGAAGAATAGAGGACGAATACAAGGT TATGAGGTTAAATATTGGAGACATGAAGACAAAGAAGAAAATGCTAGAAAAATACGAACAGTTGGAAAT CAGACATCAACAAAAATCACGAACTTAAAAGGCAGTGTGCTGTATCACTTAGCTGTCAAGGCATATAAT TCTGCTGGGACAGGCCCCTCTAGTGCAACAGTCAATGTGACAACCCGAAAGCCACCACCAAGTCAACCC CCCGGAAACATCATATGGAATTCATCAGACTCCAAAATTATCCTGAATTGGGATCAAGTGAAGGCCCTG GATAATGAGTCGGAAGTAAAAGGATACAAAGTCTTGTACAGATGGAACAGACAAAGCAGCACATCTGTC ATTGAAACAAATAAAACATCGGTGGAGCTTTCTTTGCCTTTCGATGAAGATTATATAATAGAAATTAAG CCATTCAGCGACGGAGGAGATGGCAGCAGCAGTGAACAAATTCGAATTCCAAAGATATCAAATGCCTAC GCGAGAGGATCTGGGGCTTCCACTTCGAATGCATGTACGCTGTCAGCCATCAGTACAATAATGATTTCC CTCACAGCTAGGTCCAGTTTATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001206956
Insert Size	2094 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206956.1
RefSeq Size	3942 bp
RefSeq ORF	2094 bp
Locus ID	152330
UniProt ID	Q8IWV2
Protein Families	Secreted Protein
MW	76.5 kDa
Gene Summary	This gene encodes a member of the contactin family of immunoglobulins. Contactins are axon-associated cell adhesion molecules that function in neuronal network formation and plasticity. The encoded protein is a glycosylphosphatidylinositol-anchored neuronal membrane protein that may play a role in the formation of axon connections in the developing nervous system. Deletion or mutation of this gene may play a role in 3p deletion syndrome and autism spectrum disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011] Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream in-frame start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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